Assessment of phylogenetic relationship of rare plant species collected from Saudi Arabia using internal transcribed spacer sequences of nuclear ribosomal DNA

Al‐Qurainy, Fahad; Khan, Salim; Nadeem, Mohammad; Tarroum, Mohamed; Alaklabi, Abdullah

doi:10.4238/2013.march.11.21

Cited by 9 publications

(4 citation statements)

References 29 publications

(21 reference statements)

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“…The study revealed a high level of genetic diversity among the Ajuga arabica populations in Saudi Arabia. This finding is consistent with previous studies on other plant species in Saudi Arabia, which have also reported high levels of genetic diversity (Al-Qurainy et al, 2013;Al-Rashedi et al, 2018). The high genetic diversity of Ajuga arabica populations in Saudi Arabia can be attributed to a number of factors, including its geographical location, climatic conditions, and historical events.…”

Section: Discussionsupporting

confidence: 93%

Population Structure and Genetic Diversity of <em>Ajuga Arabica</em> in Saudi Arabia: A Comparative Analysis of DNA and Gene Sequences

Odah

2024

Preprint

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Ajuga arabica is an essential medicinal plant found naturally in Saudi Arabia, recognized for its therapeutic characteristics. Nevertheless, research on the genetic diversity and population arrangement of this species in Saudi Arabia is deficient. In this study, we conducted a comparative examination of DNA and gene sequences to evaluate the genetic diversity and population configuration of Ajuga arabica in Saudi Arabia. Our findings demonstrate that there are high levels of genetic diversity within populations, but low levels of genetic differentiation among populations. Moreover, we discovered proof of gene flow among populations, suggesting that there are no significant genetic barriers to gene flow. Our results offer valuable insights into the genetic diversity and population structure of Ajuga arabica in Saudi Arabia, which can support conservation efforts and promote the sustainable use of this valuable medicinal plant.

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Section: Discussionsupporting

confidence: 93%

Population Structure and Genetic Diversity of <em>Ajuga Arabica</em> in Saudi Arabia: A Comparative Analysis of DNA and Gene Sequences

Odah

2024

Preprint

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“…The ITS markers have already been used successfully for identification of Ochradenus arabicus (Resedaceae), an endemic medicinal plant of Saudi Arabia (Khan et al, 2012). Similarly, phylogenetic relationship was worked out using the nrDNA-ITS sequence in Senecio asirensis, Nepeta deflersiana and some rare species of Saudi Arabia (Khan et al, 2013;Al-Qurainy et al, 2013;. The development of ITS marker is easy, reproducible and can be developed from a small amount of tissue.…”

Section: Resultsmentioning

confidence: 99%

Identification and reconstruction of phylogeny among three species of Cleome using nrDNA-ITS sequence

Khan

Al‐Qurainy

Nadeem

et al. 2022

Int. J. Appl. Exp. Biol.

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Three species of genus Cleome, C. arabica, C. ramosissima and C. amblyocarpa were identified using nuclear ribosomal DNA internal transcribed spacer sequence (nrDNA-ITS). The phylogenetic tree was made using different methods viz., neighbor-joining (NJ), maximum parsimony (MP), and maximum likelihood for accuracy of the results, and congruence finding in phylogeny. Cleome ramosissima and C. amblyocarpa were found to be clustered in one group according to the sequence similarity. However, C. arabica clustered in a separate clade. The nrDNA-ITS marker clearly differentiated and showed phylogenetic relationships among the three species. Thus, nrDNA-ITS marker could be effectively used for other species of Cleome for their identification and phylogenetic studies.

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“…Results revealed the proximity and maximal identity of Resedae with 100% bootstrap support and nested within the clade. Al-Qurainy et al (2013, 2014a,b) analyzed five species of rare and endangered plants from Saudi Arabia. Al-Qurainy et al (2014a,b) evaluated medicinal plants Nepeta deflersiana and Plectranthus asirensi using DNA barcoding.…”

Section: Plant Dna Barcoding: An Emerging Approachmentioning

confidence: 99%

The Promise of Molecular and Genomic Techniques for Biodiversity Research and DNA Barcoding of the Arabian Peninsula Flora

et al. 2019

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The Arabian Peninsula is known to have a comprehensive and rich endowment of unique and genetically diverse plant genetic resources. Analysis and conservation of biological diversity is a crucial issue to the whole Arabian Peninsula. The rapid and accurate delimitation and identification of a species is crucial to genetic diversity analysis and the first critical step in the assessment of distribution, population abundance and threats related to a particular target species. During the last two decades, classical strategies of evaluating genetic variability, such as morphology and physiology, have been greatly complemented by phylogenetic, taxonomic, genetic diversity and breeding research molecular studies. At present, initiatives are taking place around the world to generate DNA barcode libraries for vascular plant flora and to make these data available in order to better understand, conserve and utilize biodiversity. The number of herbarium collection-based plant evolutionary genetics and genomics studies being conducted has been increasing worldwide. The herbaria provide a rich resource of already preserved and identified material, and these as well as freshly collected samples from the wild can be used for creating a reference DNA barcode library for the vascular plant flora of a region. This review discusses the main molecular and genomic techniques used in plant identification and biodiversity analysis. Hence, we highlight studies emphasizing various molecular techniques undertaken during the last 10 years to study the plant biodiversity of the Arabian Peninsula. Special emphasis on the role of DNA barcoding as a powerful tool for plant biodiversity analysis is provided, along with the crucial role of herbaria in creating a DNA barcode library.

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Assessment of phylogenetic relationship of rare plant species collected from Saudi Arabia using internal transcribed spacer sequences of nuclear ribosomal DNA

Cited by 9 publications

References 29 publications

Population Structure and Genetic Diversity of <em>Ajuga Arabica</em> in Saudi Arabia: A Comparative Analysis of DNA and Gene Sequences

Population Structure and Genetic Diversity of <em>Ajuga Arabica</em> in Saudi Arabia: A Comparative Analysis of DNA and Gene Sequences

Identification and reconstruction of phylogeny among three species of Cleome using nrDNA-ITS sequence

The Promise of Molecular and Genomic Techniques for Biodiversity Research and DNA Barcoding of the Arabian Peninsula Flora

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