Assessment of plasma nitric oxide concentration and erythrocyte arginase activity in dairy cows with traumatic reticuloperitonitis

Abstract

The aim of this study was to evaluate plasma nitric oxide (NO) concentrations, erythrocyte arginase (ARG) activity, plasma fibrinogen (Fb) and serum iron (Fe) levels and some biochemical parameters in dairy cows with traumatic reticuloperitonitis (TRP). The animal material of the study consisted of 14 Swiss Brown cows diagnosed with TRP (TRP group) between 4-8 years old brought to Firat University Animal Hospital Clinics and 14 healthy Swiss Brown cows (control group) aged 4-8 years obtained from dairy farms in different regions. Blood samples were taken from the vena jugularis of the animals. Concentrations of plasma NO, Fb, erythrocyte ARG activity, and some biochemical markers were determined after the serum and plasma of the receiving blood were separated. While the NO (318.9±5.8 vs. 270.3±9.6 μmol/L) concentrations of the TRP group were found to be significantly higher than the control group (P<0.001), the erythrocyte ARG activity (29.5±0.5 vs. 35.2±1.0 U/hb) was found to be higher in the control group (P<0.001). It was also observed that total protein (TP) (6.6±0.5 vs. 7.8±0.1 g/dL) (P<0.05) and Fb (914.3±68.6 vs. 265.4±19.8 mg/dL) (P<0.001) concentrations were higher in the TRP group, compared to the control group, while albumin (ALB) (1.9±0.2 vs. 3.1±0.1 g/dL) and Fe (47.00±5.29 vs.106.79±9.44 μg/dL) concentrations were significantly lower than the control group (P<0.001). In addition, a positive correlation was found between NO and Fb concentrations and between erythrocyte ARG activity and Fe concentrations. As a result, it was determined that NO concentrations were increased and erythrocyte ARG activity was not significant in dairy cows with TRP. In addition, increased plasma Fb concentration and decreased serum Fe concentration were determined in dairy cows with TRP. This study demonstrated that plasma NO, Fb and serum Fe concentrations in dairy cows with TRP may be useful markers for prognosis.

Exploration of two-dimensional bio-functionalized phosphorene nanosheets (black phosphorous) for label free haptoglobin electro-immunosensing applications

“…Haptoglobin (Hp) is an important bovine acute phase protein that is stimulated by inflammatory mediators and associated with an initial reaction to infection, inflammation, or trauma in beef and dairy herds [13]. The level of Hp circulating in blood or serum is a strong indicator of inflammation, and its elevated concentration (amount) is associated with infection and inflammation, is an important clinical sign of traumatic reticuloperitonitis [14], and has a strong association with cows with acute puerperal metritis [15][16][17]. Hp concentrations of more than 0.2 mg ml −1 indicate mild infection, and values above 0.4 mg ml −1 suggest severe infection, while extended pathological conditions are typically associated with Hp values in the range of 1-2 mg ml −1 [18].…”