Some biodiversity of global importance are found in the Niger Delta region of Nigeria. The biodiversity resources of the Niger Delta comprises of several species of mammals, reptiles, amphibians, avian fauna, microorganisms, fisheries (animals), and mosses, liverworts, pteridophytes, gymnosperms, chlamydosperms, monocotyledons, dicotyledons etc (plants). The region is hotspots of several endemic species. In recent times, the biodiversity distribution with regard to abundance and diversity appears to be on the decline trend. Hence, this study assessed the factors leading to decline of biodiversity resources in the Niger Delta. The study found that excessive exploitation, urbanization/industrialization, deforestation/habitat destruction, bush burning, soil erosion and pollution, climate change are the leading cause of loss of biodiversity. The loss of biodiversity affects its roles including source of medicinal plants, shelters, habitats, raw materials for several art and construction works (plants), nutrient mineralization through biogeochemical cycles, loss of animal protein and species of global importance. The paper concludes by suggesting full implementation and enforcement of several environmental conventions, laws and legislations geared towards protection of biodiversity at both international and national level of which Nigeria is part of. Furthermore, outlining biodiversity conservation strategies in curriculum of school is another potential option for conserving biodiversity.