Assessment of poly-l -lysine dendrigrafts for virus concentration in water: use of MS2 bacteriophage as proof of concept

Abstract

Aims: Virus detection has often been difficult due to a low concentration in water. In this study, we developed a new procedure based on concentration of virus particles on an innovative support: poly-L-lysine dendrigrafts (DGL), coupled with directed nucleic acid extraction and real-time PCR quantification. Methods and Results: This method was evaluated using the bacteriophage MS2 as a model virus. This virus exhibited the size and structural properties of human pathogenic enteric viruses and has often been used to assess new supports of concentration. Moreover, this bacteriophage is also a faecal contamination indicator. In this study, many water filtration conditions were tested (volume of water, concentration, etc.), and more than 80% of bacteriophage were recovered after filtration on polymer, in most conditions. We demonstrated that the method was linear (slope = 0Á99 AE 0Á04 and Y intercept when x = À0Á02 AE 0Á28), valid (as manipulators, tested concentrations, volumes of sample and batch of polymer did not have any influence on concentration) and sensitive (allowing to concentrate up to 16 600-fold 1 l of sample and to detect and quantify down to 750 GC l À1 and 7500 GC l À1 , respectively).Conclusions: To conclude, this support exhibits high interest to retain viruses and to allow to detect low concentration of virus in water. Significance and Impact of the Study: This study gives valuable advance in the methods of concentration and diagnosis of virus in water.

Digitizing Poly-l-lysine Dendrigrafts: From Experimental Data to Molecular Dynamics Simulations

“…In contrast to PAMAM and PEI, DGLs are biodegradable, 2 exhibit low cellular toxicities, 3 and turned out to be non-immunogenic. 4 As a consequence, DGLs have recently gained a huge interest in the biomedical field during the last quinquennium, with numerous applications in drug and gene delivery, 2,3,[5][6][7][8][9][10][11][12][13][14][15][16][17][18] biomaterials and tissue engineering, [19][20][21][22][23][24][25] bio-imaging [26][27][28][29][30][31] and biosensing. [32][33][34] Despite this growing use of DGLs, a deeper understanding of the molecular level properties of these macromolecules is missing.…”

Everything You Always Wanted to Know about Poly‐l‐lysine Dendrigrafts (But Were Afraid to Ask)

“…[51] In addition, DGL G4,i na ssociation with ap oly(e-caprolactone) membrane, was also an effective nanoreservoir for the nerve growth factor;t he resulting biomaterial being able to induce the full vascularization and innervation of atooth in vivo. [53] This approachr elies on the non-covalenti nteractions that take place between positively charged dendrigraftsa nd the negatively charged surface of the viral particles. A) Schematic illustration of the G3-based theragnostic nanoparticle(Dox/Mito-DGL) structure, and the mechanism of mitochondria-targeting drug release triggered by ATP.…”

“…[52] Biosensing In ab iosensing purpose,athird-generation generation DGL G3,i mmobilizedo np lasma activated polypropylene filters, was reported to be an efficient capture agentf or concentrating bacteriophage MS2, allowingt he quantification of the model virus in large volumes of water. [53] This approachr elies on the non-covalenti nteractions that take place between positively charged dendrigraftsa nd the negatively charged surface of the viral particles. It is interesting to note that, since DGLs and bacteria also displayed high affinities based on electrostatic interactions, [33] this approachc ould be extended to the detection and/orr emoval of pathogenic bacteria from contaminated waters.R ecently,V iale tal.…”

Computer simulation of complex of lysine dendrigraft with molecules of therapeutic KED peptide

“…Lysine dendrigrafts are polymers that are rich with amines. Due to this reason they could be used as antibacterial [7] or antiviral agents [8]. Also they could make complexes with oppositely charged peptides due to strong electrostatic interaction between their positively charged groups (NH3+) and negatively charged aminoacid side groups (COO-) of peptides.…”