Assessment of Pregnant Women Toxoplasmosis by ELISA Method in Miandoab City, Iran

Garedaghi, Yagoob; Firozivand, Yaghoub

doi:10.15296/ijwhr.2017.13

Cited by 6 publications

(4 citation statements)

References 9 publications

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“…22 Similarly, in Iran, reported positive IgG and IgM titers of 39.5% and 2.5%, in the sera of the pregnant women, respectively. 30 The seroprevalence rate of toxoplasmosis can be affected by many factors for instance the nature of environment surroundings, food and drinks sources, and the hygienic conditions. These outcomes agreed slightly with results recorded the higher rates of seropositive toxoplasmosis among housewives followed by employee then the student of about 50%, 40.9% and 6.8% respectively.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence and some demographic factors associated with Toxoplasma gondii infection among female population in Duhok province, Iraq

Salih

Mero²,

Eassa

2020

Int J Res Med Sci

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Background: The causative agent of toxoplasmosis is Toxoplasma gondii which is an intracellular protozoan. It has an important role in abortion and congenital diseases in pregnant women, which lead to infant's defectiveness birth when pregnant, are exposed during pregnancy. Toxoplasmosis is one of the most prevalent and most successful parasitic infectious disease worldwide, due to its efficient transmission through the ingestion of tissue cysts in undercooked and infected meat, or the ingestion of oocysts in contaminated vegetables and water. The present study aimed to investigate the seroprevalence rate of anti-Toxoplasma gondii Abs and their relation to some demographic factors among females, in Duhok province/ Kurdistan Region/ Iraq.Methods: During the period from October 2016 to November 2017, a total of 792 random blood samples were collected from the female population of different ages (16-55) years and various socioeconomic classes, who attended Obstetrics and Gynecology Hospital, Azadi teaching hospital and Central Public Health Laboratory/ Duhok.Results: Out of 792 samples examined, 288 (36.3%) were seropositive from which 282 (35.61%) were found seropositive for IgG, while only six samples (0.76%) were seropositive for IgM. Regarding to occupation, the highest rate for chronic toxoplasmosis was reported in housewives followed by employed and students at rates of 40.19%, 22.3%, and 14.0%, respectively. These outcomes were statistically significant (p<0.05). The age group 36-45 years showed the highest seropositive rate for both IgG and IgM Abs which were 41.02% and 1.28%, respectively, which was statistically non-significant. The higher rate of seropositivity was observed among married females 39.93%, and pregnant 41.9% versus non-pregnant 33.3%. This difference was statistically significant (p<0.05) among married while non-significant in pregnant. Concerning to blood groups, the highest rate of seropositivity was reported among females with blood group AB+ which was 60% followed by group O- which was 46.7 %. This difference was statistically non-significant (p>0.05). Conclusions: This study showed the importance of demographic factors to the epidemiology of T. gondii in females, which support the role of public health in the control of infectious diseases.

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Section: Discussionmentioning

confidence: 99%

Seroprevalence and some demographic factors associated with Toxoplasma gondii infection among female population in Duhok province, Iraq

Salih

Mero²,

Eassa

2020

Int J Res Med Sci

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“…Immunochromatography (rapid test) was used during this study as a diagnostic tool for determining antibodies produced in response to T. gondii antigens.The presence of IgG antibodies indicates chronic infection with counterattacks, whereas the presence of IgM antibodies indicates an acute infection; the presence of IgG and IgM antibodies together indicates the occurrence of stimulation of the chronic infection and its transformation into an acute infection; and the cause of the presence of IgG is greater than the IgG rate. Adding IgM until the IgM disappears or decreases to extremely low levels, followed by the appearance of IgG that persists for a prolonged duration [21] The correlation of LTF with IL-17A and TNF-α parameters of the current research can provide more Positive correlation indicates that the two parameters are positively correlated both of them increase together. But negative correlation denotes that the two parameters are inversely correlated, it means an increase in one variable leads to a decrease in the other.…”

Section: Discussionmentioning

confidence: 59%

Biomarker Evaluation in Toxoplasma gondii-infected Patients

Sabah,

Abed

2024

J. Res. Appl. Sci. Biotechnol.

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Back ground: This study used Toxoplasma IgG/IgM Antibody Rapid test to detect Toxoplasmosis in pregnant women and evaluate their lipid profile compared to a healthy control group. The aim of the study: Serodiagnosis of parasite infection in aborted pregnant women using the rapid detection method. Evaluating the level of some immunological parameters in infected women such as IL17-A, TNA-, lactoferrin, shedding light on the lipid profile of parasite-infected women. Patients and Methods: blood samples 240 were collected from aborted pregnant women attending some governmental hospitals and private medical clinics in some areas of Salah al-Din Governorate during the period from 1/7/2022 to 1/2/2023 and the required information was recorded according to a special questionnaire form. The results: were analyzed statistically using the IBM SPSS Statistics (version 23) program, according to the t-test, at a significant level of p≤0.05. The results of Rapid Test (93 positive samples) 38.7% (divided into 80) 86.02% (positive samples for IgG, while 13 sample) 13.97% (positive for IgM and 147 negative samples) 61.25%. (There is a positive correlation between (LTF, IL-17A, TNF-a) (highly significant). The lipid profile analysis revealed a non-significant increase for each of cholesterol and high-density lipoprotein (HDL) in infected women, as Mean ± S.E concentration was (168.4±8.2 Pg/ml); (33±1.5pg/ml) respectively, compared to the control group, where the Mean ± S.E concentration was (141.6±15.5 Pg/ml); (28±3.09Pg/ml) respectively. While there was a non-significant decrease in each of LDL, very low-density lipoprotein (VLDL), and triglycerides in pateints group, as Mean ± S.E concentration was (80.6±3.7pg/ml); (45.4±1.07 pg/ml); (159.7±4 pg/ml), respectively, compared to the control group, where the average concentration was (84.3±5.3pg/ml); (48.6±2pg/ml) 161.3±6.7pg/ml) respectively.

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“…In two surveys in West Azerbaijan Province, one in Urmia and one in Miandoab city, on pregnant women who kept cats at home or had a history of contact with cats, positive cases of IgG and IgM antibodies were identified, but the correlation was insignificant [ 12 , 32 ]. In a further study conducted in Urmia city, the seroprevalence was not related to contact with cats [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and risk factors of Toxoplasma gondii infection among women with miscarriage and their aborted fetuses in the northwest of Iran

Zeinali,

Khademvatan,

Jafari

et al. 2023

PLoS ONE

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Toxoplasmosis is a worldwide disease of various animals and human and results from infection with the Toxoplasma gondii parasite. Abortion and congenital defects are important consequences of the T. gondii infection. The aim of this study was to determine the Toxoplasma-induced abortions among women with miscarriage and the presence of T. gondii in their aborted fetuses in Urmia, the northwest of Iran. This cross-sectional study was conducted with 215 women with abortion and their aborted fetuses, from 2020 to 2021. Seroprevalence of anti-Toxoplasma IgG and IgM were determined using the sera of the aforesaid women. Nested PCR was carried out using RE-529 gene sequences, and sequencing was performed using the T. gondii GRA6 gene on the remnant of pregnancy after abortion. The tissue positive samples were then subjected to another PCR on GRA6 gene and sequenced for genotyping. Among 215 serum samples of women with abortion, 70 (32.6%) were positive for anti-Toxoplasma IgG, and three (1.4%) were positive for IgM. The RE-529 sequence of T. gondii was positive in three (1.4%) of the aborted fetuses. The analysis of GRA6 gene indicated that all three positive samples carried a GRA6 allele (GRA6I) of T. gondii type I genotype. Our findings suggest that T. gondii is one of the causative agents of spontaneous abortion in West Azerbijan Province, the northwest of Iran.

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Assessment of Pregnant Women Toxoplasmosis by ELISA Method in Miandoab City, Iran

Cited by 6 publications

References 9 publications

Seroprevalence and some demographic factors associated with Toxoplasma gondii infection among female population in Duhok province, Iraq

Seroprevalence and some demographic factors associated with Toxoplasma gondii infection among female population in Duhok province, Iraq

Biomarker Evaluation in Toxoplasma gondii-infected Patients

Prevalence and risk factors of Toxoplasma gondii infection among women with miscarriage and their aborted fetuses in the northwest of Iran

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