Assessment of prevalence of Fasciola hepatica and associated biochemical alterations in the cattle of Siirt province, Turkey

Çelik, Özgür; Çelik, Burçak Aslan; Irak, Kıvanç; Akgül, Gülşah

doi:10.18805/ijar.b-1058

Cited by 3 publications

(3 citation statements)

References 9 publications

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“…Although the current study outcome was lower than the rates obtained in Iran, which was 32% 37 . In contrast, the prevalence was lower than 7.5 % reported in Turkey 38 .…”

Section: Epidemiological Studycontrasting

confidence: 67%

Histopathologic Changes and Molecular Characterization of Fascioliasis (a Zoonotic Disease) among Slaughtered Livestock in Erbil and Halabja Abattoirs, Kurdistan Region-Iraq

Koyee,

Khailany,

Rahman

et al. 2024

Baghdad Sci.J

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Fascioliasis is a zoonotic disease, caused by the parasites Fasciola (F.) hepatica and F. gigantica, which poses a great threat to the ruminants in addition to humans in many countries, including Iraq. Hence, the study of this parasite and its deleterious impacts on host, morphologically, histopathologically, epidemiologically, and molecularly, is so important. Hence, the current study was designed to investigate this fluke of livestock in Erbil and Halabja slaughterhouses from August to October 2022. To meet the prerequisites of the study, 33 flukes were collected from the sheep and cattle at study sites. The samples were transported to the Zoology Research Laboratory, Salahaddin University-Erbil, Iraq. Some of them were used for morphological identification, and others were preserved in 96% ethanol and stored at -20°C. Subsequently, DNA was extracted. Conversely, pieces of the infested liver were fixed in 99% ethanol and 10% formalin before histopathologic examination. The parasite species were identified using traditional Polymerase Chain Reaction (PCR), and sequencing methods. Macroscopically, hepatomegaly was the main finding of the infestation. Among livestock, cattle exhibited the highest rate of morbidity, followed by goats and sheep. The amplified DNA region was 98–99%, matched to F. hepatica and F. gigantica sequences. It was also established that 28S rDNA sequencing combined with morphologic characteristics of Fasciola species can be applied as a salient indicator in the identification of flukes. The current study is reckoned as a comprehensive investigation regarding fascioliasis, because it covered the parasite epidemiology, morphology, and molecular identification, despite of histopathologic examination.

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“…Although the current study outcome was lower than the rates obtained in Iran, which was 32% 37 . In contrast, the prevalence was lower than 7.5 % reported in Turkey 38 .…”

Section: Epidemiological Studycontrasting

confidence: 67%

Histopathologic Changes and Molecular Characterization of Fascioliasis (a Zoonotic Disease) among Slaughtered Livestock in Erbil and Halabja Abattoirs, Kurdistan Region-Iraq

Koyee,

Khailany,

Rahman

et al. 2024

Baghdad Sci.J

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“…(Rahman et al, 2017). The odds of fascioliasis positivity in female goats and sheep was 2.7918 times (95% CI: 1.7314-4.5017, P < 0.05) and 3.7317 times (95% CI: 1.7314-4.5017, P < 0.05) respectively than male goats and sheep which is higher compared to the level of odds obtained in previously published reports (Aktaruzzaman et (Çelik Ö and Aslan Çelik, 2018). This study also revealed that there was a signi cant association between body condition score and fascioliasis in sheep and goats.…”

Section: Prevalence Of Fascioliasis In Slaughtered Sheepmentioning

confidence: 53%

An insight into the epidemiology of foodborne zoonotic fascioliasis in small ruminants in northwestern region of Bangladesh

TALUKDER,

Ahmed,

ISLAM

et al. 2024

Preprint

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Fascioliasis is one of the most common foodborne zoonotic infection of ruminants in Bangladesh. To estimate the prevalence and associated risk factors of fascioliasis and extent of liver damage, 825 livers of sheep and goats were randomly inspected during onsite slaughterhouse visiting in Naogaon, Natore, Rajshahi and Joypurhat districts. The overall prevalence of fascioliasis was 25.09% and significantly (P = 0.008) higher in goats (26.11%) than sheep (24.00%). During gross inspection, Fasciolainfected livers were increased in size, fibrosed, fatty, multiple white or reddish necrotic foci on the parietal surface, hard to cut, calcified, and numerous mature and immature flukes were also observed. In histoarchitecture, inflammatory cell infiltration in the hepatic parenchyma and periportal area, fibrous connective tissue proliferation around necrotic area, hyperplastic bile duct, congestion, and primary biliary cirrhosis were seen in acute and chronic fascioliasis. Epidemiological investigations revealed that fascioliasis was higher in goats than sheep. Age, sex, BCS and season were found to have statistically significant associations with fascioliasis in goats. In case of sheep, age (OR = 5.8671; 95% CI: 2.9482 - 11.6757, P < 0.0001), sex (OR = 3.7317; 95% CI: 1.9052 - 7.3094, p < 0.0001), BCS (OR = 6.0346; 95% CI: 1.7986 - 20.2472, p <.0001), and season (OR = 8.2308; 95% CI: 3.9922 - 16.9693, p = <.0001) were also found to have statistically significant associations with fascioliasis. Results of the study can help for molecular epidemiology of fascioliasis in small ruminants to plan fluke control programs for safe food production.

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“…The liver fluke of the genus Fasciola is an extremely pathogenic parasite that affects humans and animals and causative agent of fascioliasis ( 1 ). Animal liver infection with Fasciola species results a large economic loss ( 2 ). This trematode directly affects livestock productivity through host mortality (in sheep) and partial or complete liver condemnation.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Liver Fluke Isolated from Sheep, Goat and Cattle in Sulaymaniyah, Iraq

Othman,

Hama,

Zorab

et al. 2023

IJPA

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Background: We aimed to determine species of liver fluke that predominately cause fascioliasis in sheep, goats, and cattle in the Sulaymaniyah Province, Iraq using the molecular technique of DNA sequencing and restriction fragment length polymorphism (RFLP). Methods: The samples were collected from November 2021 to May 2022. The flukes were collected from infected livers of livestock at the slaughterhouse of Sulaymaniyah Governorate, Iraq. A total of 205 flukes were collected from 56 hosts, cattle (n=22), sheep (n=28), and goats (n=6). The specific primers for FCOX1 and 28S rDNA gene amplification were used. The PCR products were subjected to restriction fragment polymorphism (RFLP) assay using Hpy188III and Dra II restriction enzymes, besides DNA sequencing. Results: The results showed the genetic polymorphisms among the flukes. Three patterns of RFLP were observed Fasciola hepatica, F. gigantica, and F. intermediate, where 28 of them displayed F. hepatica (sheep, n=14, goat, n=3 and cattle, n= 11), whereas 24 samples displayed the F. gigantica (sheep, n=12, goat, n=3 and cattle, n= 9), and only four samples belonged to F. intermediate (sheep n=3 and cattle, n=1). In addition, the result of the ribosomal DNA (28S rDNA) sequencing confirmed that the isolated flukes belonged to F. hepatica, F. gigantica and F. intermediate. Conclusion: All three main species are present in the study area and F. hepatica predominated among the animal species in this area also, our results concluded that PCR-RFLP is a rapid and reliable method for liver fluke species identification.

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Assessment of prevalence of Fasciola hepatica and associated biochemical alterations in the cattle of Siirt province, Turkey

Cited by 3 publications

References 9 publications

Histopathologic Changes and Molecular Characterization of Fascioliasis (a Zoonotic Disease) among Slaughtered Livestock in Erbil and Halabja Abattoirs, Kurdistan Region-Iraq

Histopathologic Changes and Molecular Characterization of Fascioliasis (a Zoonotic Disease) among Slaughtered Livestock in Erbil and Halabja Abattoirs, Kurdistan Region-Iraq

An insight into the epidemiology of foodborne zoonotic fascioliasis in small ruminants in northwestern region of Bangladesh

Molecular Characterization of Liver Fluke Isolated from Sheep, Goat and Cattle in Sulaymaniyah, Iraq

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