Assessment of Quercetin Content in Selected Vegetables and Fruits by Conventional Extraction and High Performance Liquid Chromatography

Abstract

One of the dietary flavonoids which can be found in a variety of vegetables and fruits is Quercetin (3,3′,4,5,7-pentahydroxyflavone).Quercetin reduce infection risk and also has unique biological property which improves the physical performance. The current research work describes the extraction and characteristic of quercetin present in carrot (Daucus carota sp. sativus) and grapes (genus vitis). A liquid – solid extraction method of quercetin contained in carrot and grapes was developed, in which Quercetin is extracted from a solid mixture using a liquid solvent (methanol). Determination of Quercetin is studied by using High performance liquid chromatography. The separation study was performed on Zodiac C18, 250mmx 4.6mm, 5µm column, detection at 280nm and flow rate applied 1mL/min. The limits of detection(LOD) and quantification(LOQ) parameters were in the ranges of 0.1–0.3 and 0.3–1.0 μ g/ mL respectively. The results of carrot and grape meet the specified specification limit. The detection of the active substance in carrot and grapes using the HPLC method has the advantage of being simple, fast, and accurate and the reported method was validated.