Assessment of Reactivity to Tumor Extracts by Leukocyte Adherence Inhibition and
Dermal Testing2

Burger, Denis R.; Vandenbark, Arthur A.; Finke, Patricia; Malley, Arthur; Frikke, Maureen J.; Black, J. I. Munro; Acott, K. M.; Begley, D.; Vetto, R. Mark

doi:10.1093/jnci/59.2.317

Cited by 39 publications

(7 citation statements)

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“…[10,11]. The assay has generated considerable interest because of its simplicity, sensitivity, and correlation with the in vivo events of tumor growth and immunity [12,13]. The LAI procedure was introduced as an improvement of the migration inhibition assay [10]; however, studies of the mechanism of LAI indicate that both specific cytophilic antibody [141 as well as a non-Ig lymphokine [15][16][17][18] mediate the reaction, making it distinct from migration inhibition.…”

mentioning

confidence: 99%

Suppression of the Leukocyte Adherence Inhibition Response to Murine-Sarcoma-Virus-Induced Tumors by Tumor-Associated Suppressor Cells

Mortensen¹,

Elson²

1980

Oncology

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The tumor-specific leukocyte adherence inhibition (LAI) response of lymphoid cells from C57B1/6 mice sensitized to a murine-sarcoma-virus (MuSV)-induced tumor was inhibited by adherent phagocytic spleen cells from mice with progressively growing RBL-5 tumors. Both the direct LAI response and the formation of the mediator of LAI were sensitive to the suppressive activity of the progressor spleen macrophages. Suppression of LAI was not restricted by H-2 differences between the sensitized lymphocytes and progressor macrophages. Suppression was also nonspecific since the antibody plaque-forming cell response of normal lymphocytes to triphenylated hemocyanin was lowered by the addition of progressor spleen macrophages. Removal of adherent suppressor macrophages during the late stages of tumor growth revealed greatly elevated LAI reactivity not detectable in an unfractionated population.

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mentioning

confidence: 99%

Suppression of the Leukocyte Adherence Inhibition Response to Murine-Sarcoma-Virus-Induced Tumors by Tumor-Associated Suppressor Cells

Mortensen¹,

Elson²

1980

Oncology

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“…Evidence of spontaneous anti-tumor response both by in vivo and in vitro reactivity was reported by several investigators employing soluble autologous or allogeneic tumor extracts by skin testing (Herberman, 1973;Char et al, 1974;Burger et al, 1977); leucocyte migration inhibition (LMI) (Andersen et al, 1970;Black et al, 1974); and leucocyte adherence inhibition (LAI) (Flores et al, 1977).…”

Section: Discussionmentioning

confidence: 99%

Direct endolymphatic instillation (ELI) of BCG-Tumor cell mixture

Stein

Adler

Goldfarb

et al. 1978

Cancer Immunol Immunother

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“…The entire procedure requires only 3 -4 h to complete. Because of the brevity, simplicity, and the fact that tumor cells need not be separated, cultured or labelled, the original method has been improved in some ways and used to demonstrate tumor-specific reactivities of leukocytes from patients with breast [2,13), colorectal [11,17], pancreas [8] or prostatic can cer [18] or malignant melanoma [10,[14][15][16], The LAI microtest [9] is one of the improved methods. The original LAI technique by Holliday et al [1] requires careful floatation of a cover slip from the surface of a haemocytometer, but in the microtest the plate is only held in an inverted position to allow nonadherent cells to float off the bottom of the wells.…”

Section: Discussionmentioning

confidence: 99%

Leukocyte Adherence Inhibition Microtest for the Detection of Cell-Mediated Immunity to Tumor in Gastric Cancer Patients

Koyama¹,

Sakita²,

Fukutomi³

et al. 1981

Oncology

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The reactivities of leukocytes from gastric cancer and noncancer patients to gastric tumor and normal tissue extracts were tested by the leukocyte adherence inhibition (LAI) microtest, assessing cell-mediated immunoreactivity to adenocarcinoma of the stomach. The reactivities were expressed with the LAI index. All leukocyte preparations showed low reactivities, a LAI index of less than 20%, to normal tissue extracts and only the preparations of leukocytes from cancer patients displayed high reactivities, a LAI index of more than 20%, to tumor extracts. Assuming that a patient is sensitized to gastric tumor antigen if his leukocytes respond to at least one tumor extract with a LAI index of more than 20%, approximately half of the cancer patients tested in the present study are considered to have positive cell-mediated immunoreactivity to gastric tumor antigen. Thus, the LAI microtest appears to be a simple, rapid and specific method for demonstrating cell-mediated immunity to tumor.

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Assessment of Reactivity to Tumor Extracts by Leukocyte Adherence Inhibition and Dermal Testing2

Cited by 39 publications

References 0 publications

Suppression of the Leukocyte Adherence Inhibition Response to Murine-Sarcoma-Virus-Induced Tumors by Tumor-Associated Suppressor Cells

Suppression of the Leukocyte Adherence Inhibition Response to Murine-Sarcoma-Virus-Induced Tumors by Tumor-Associated Suppressor Cells

Direct endolymphatic instillation (ELI) of BCG-Tumor cell mixture

Leukocyte Adherence Inhibition Microtest for the Detection of Cell-Mediated Immunity to Tumor in Gastric Cancer Patients

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