Assessment of Red Blood Cell Deformability by Centrifugal Sedimentation

Albalak, Ariela; Streichman, Sara; Marmur, Abraham

doi:10.1080/00986449608936552

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…Using the empirically-derived blood viscosity, we previously demonstrated computational fluid dynamics-derived average peak shear stress increases from 4.0 ± 0.1dynes·cm −2 at 20 hpf to 29 ± 8 dynes·cm −2 at 60 hpf to 81 ± 11dynes·cm −2 at 120 hpf. Due to red blood cell deformation and lysis during centrifugation, we did not measure blood with high hematocrit 25 , 26 . Our current capillary pressure-based micro-channel further provides an experimental basis to determine the temporal variations in shear stress during cardiac development.…”

Section: Discussionmentioning

confidence: 99%

A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates

Lee

Chou

Kang

et al. 2017

Sci Rep

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Blood viscosity provides the rheological basis to elucidate shear stress underlying developmental cardiac mechanics and physiology. Zebrafish is a high throughput model for developmental biology, forward-genetics, and drug discovery. The micro-scale posed an experimental challenge to measure blood viscosity. To address this challenge, a microfluidic viscometer driven by surface tension was developed to reduce the sample volume required (3μL) for rapid (<2 min) and continuous viscosity measurement. By fitting the power-law fluid model to the travel distance of blood through the micro-channel as a function of time and channel configuration, the experimentally acquired blood viscosity was compared with a vacuum-driven capillary viscometer at high shear rates (>500 s−1), at which the power law exponent (n) of zebrafish blood was nearly 1 behaving as a Newtonian fluid. The measured values of whole blood from the micro-channel (4.17cP) and the vacuum method (4.22cP) at 500 s−1 were closely correlated at 27 °C. A calibration curve was established for viscosity as a function of hematocrits to predict a rise and fall in viscosity during embryonic development. Thus, our rapid capillary pressure-driven micro-channel revealed the Newtonian fluid behavior of zebrafish blood at high shear rates and the dynamic viscosity during development.

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Section: Discussionmentioning

confidence: 99%

A Rapid Capillary-Pressure Driven Micro-Channel to Demonstrate Newtonian Fluid Behavior of Zebrafish Blood at High Shear Rates

Lee

Chou

Kang

et al. 2017

Sci Rep

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“…Measuring red cell deformability can be performed by methods based on viscosity [10], centrifugal sedimentation [11], micropipette aspiration [12], rheoscopy [13], filtration or nuclepore filtration or nuclear track microfilters [14,15], and laser diffraction or the Ektacytometer [16][17][18]. The viscosity-based method indirectly measures red cell deformability by monitoring the viscosity alteration of the red cell suspension, centrifugal sedimentation works by recording quantitatively the rate of sedimentary red cells in the centrifugal blood, micropipette aspiration by observing the relationship between the shape change of red cells and suction manipulated using a micro-operator under a microscope, and rheoscopy by observing deformation of individual red cells under a given shear stress with a microscope.…”

Section: Introductionmentioning

confidence: 99%

Research on a method for automated measurement of red cell deformability

Yu¹,

Liu²,

Zhang³

2004

Meas. Sci. Technol.

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Several existing laser diffractometric methods of measuring red cell deformability are analysed and their disadvantages are identified. By establishing the mathematical model of the diffraction image of a red cell group, a new method is presented—measurement of the greyscale of diffraction images. With numerous experiments, this method allows us to statistically obtain the deformation indices of both normal and abnormal red cells, as well as the composition of red cells in the blood and the characteristic values of specific red cells. It is demonstrated that abundant information including characteristics of red cells can be obtained with this method. This method is expected to be promising in both blood disease research and clinical application.

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