Objectives: This study aims to analyze the palatal rugae pattern in the native population of Riyadh province in Saudi Arabia to understand whether the rugae patterns are unique to these ethnic origins and determine whether these palatal rugae patterns can be used as an adjunct aid in biometric human identification. Methods: A cross-sectional descriptive study was conducted among 200 participants within the age group ranging from 18 to 35. Their rugae patterns were analyzed macroscopically (Calcorrugoscopy) to record the length, shape, direction, and unification of the rugae pattern. Findings : It was found that females had a more significant number of palatal rugae (4.83 ± 1.05) as compared with males (4.30 ± 0.93) on the right side, while a reverse trend was seen in terms of the mean length of rugae where males (1.10±0.20) showed longer palatal rugae on the left side than females (0.97±0.17). This difference was statistically significant (p=0.001). A statistically significant difference was found between the mean length of rugae on either side of the palate (p=0.001) with longer rugae on the left side (1.04±.20) than the right side (0.97 ±0.16). Furthermore, a statistically significant difference based on sex was found in straight, curved, convergent, and branched palatal rugae, in the rugae oriented towards the left side of the palate (p=0.001) and in the unification of rugae on the palate's left side (p=0.001) based on sex. Novelty: The natives of Riyadh had a unique characteristic of unification and lesser number of rugae compared to other ethnic groups in Saudi Arabian Population which helps in differentiating them from other ethnicity.