Assessment of Remineralizing Effect of Bioactive Glass Based Toothpastes: an In-Vitro Comparative Study

Abstract

Purpose:The purpose of the study was to assess the enamel remineralization potential of two bioactive glass-based toothpastes: Biomin ® based toothpaste in comparison with Novamin ® based toothpaste with sodium fluoride. Material and methods: Forty premolars were selected and divided equally into two groups named Novamin and Biomin groups after discarding their roots. First, the samples were soaked in a demineralizing solution for three days to get white spot lesion. Then, after rinsing them with deionized water, toothpastes were applied in the form of slurry twice daily, for two minutes each. The remineralization process lasted for two weeks. Environmental Scanning Electron Microscope (ESEM) was used to assess the remineralization degree.It was attached to Energy-dispersive X-ray spectroscopy (EDX) unit to measure the Ca, P weight percent and also the Ca: P for each sample. Three EDX readings were taken for each sample; baseline, after demineralization and after remineralization. Results: The results showed that both toothpastes have remineralization potential without statistically significant difference between them except in phosphate weight percent which was higher in the Biomin group. Conclusions: Within the limitations of the present study we concluded that the mineral content of the demineralized enamel significantly increase with using both Biomin and Novamin containing toothpastes twice daily for two weeks. Biomin may be more effective than Novamin in remineralization of demineralized enamel.

Effect of Biomin F Toothpaste and Diode Laser on Remineralization of White Spot Lesions (In Vitro Study)

“…The present study revealed that the mineral content following remineralization reached the baseline mineral level in both test groups. This nding contradicted the results reported by Omran et al [20] as the mean calcium mass percentage after remineralization was signi cantly lower compared to the baseline calcium mass percentage in the Biomin group. Notably, both studies employed the same Biomin F application protocol (two minutes twice daily for two weeks).…”

“…This bonding reduces the leaching of bioactive glass material [38]. Biomin F contains small bioglass particles that aid in the in ltration of remineralizing agents into subsurface lesions [20,39]. With its high phosphate content, Biomin F facilitates rapid apatite formation (within 6 hours) and contains fewer carbon impurities, thereby rendering enamel less soluble in acid [20,39].…”

“…4) (Table 1). The samples were removed from the solution and rinsed with distilled water to stop the demineralization process and to remove any residuals of the solution [20,23]. At this stage, evaluations were carried out using Scanning Electron Microscope (SEM) analysis alongside Energy Dispersive X-ray Analysis (EDX).…”

“…Then it was stored in arti cial saliva to mimic oral environment. This procedure was repeated for two weeks (Table 1) [15,20].…”

“…These alterations enhance enamel's resistance against the acid attacks. Moreover, studies have demonstrated synergistic effects between laser treatment and topical uoride application, leading to a signi cant reduction in the rate of enamel decalci cation [19,20].…”