Assessment of reproductive ageing patterns by hormonal and ultrasonographic ovarian reserve tests

Tufan, Elif; Elter, Koray; Durmuşoğlu, Fatih

doi:10.1093/humrep/deh448

Cited by 32 publications

(21 citation statements)

References 28 publications

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“…The central hypothesis is that the number of primordial follicles and the quality of oocyte cells in the ovaries decrease gradually [39, 40]. In this accelerated ovarian aging model, the number of primordial follicles was substantially decreased, whereas the number of atretic follicles was significantly increased.…”

Section: Discussionmentioning

confidence: 99%

“…Ovarian stimulation was shown to impact granulosa cell apoptosis and biological aging of the ovary [64-66]. Ovarian granulosa cell apoptosis is an important marker of ovarian aging [39]. The results of TUNEL fluorescence staining showed that apoptotic granulosa cells increased in ovarian follicles in ovaries that underwent consecutive superovulation compared to those of the control group, suggesting that follicular degeneration is accelerated by consecutive superovulation.…”

Section: Discussionmentioning

confidence: 99%

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Establishment of a Mouse Model of Premature Ovarian Failure Using Consecutive Superovulation

Nie

Dai

Zheng

et al. 2018

Cell Physiol Biochem

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Background/Aims: This study investigated the effect of consecutive superovulation on the ovaries and established a premature ovarian failure (POF) model in mice. Methods: The mouse POF model was induced by 5-15 consecutive superovulation treatments with pregnant mare serum gonadotropin (PMSG), human chorionic gonadotropin (HCG) and prostaglandin F2α (PGF2α). Normal adult mice were compared with mice displaying natural ovarian aging. The following serum biochemical parameters were measured: including follicle-stimulating hormone (FSH), luteinizing hormone (LH), progesterone (P), estradiol (E2), inhibin B (INH B), malondialdehyde (MDA), total superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels. Follicles were counted using H&E staining. Levels of 8-hydroxyguanosine (8-OhdG), 4-hydroxynonenal (4-HNE), nitrotyrosine (NTY), anti-Mullerian hormone (AMH) and CDKN2A/ p16 (p16) were detected using immunohistochemical staining. Reactive oxygen species (ROS) levels were measured using dihydroethidium (DHE) staining. Cell apoptosis was detected using an in situ TUNEL fluorescence staining assay. Levels of proteins involved in ROS-related pathways and the p16 protein were detected using Western blotting. Sod1, Sod2 and Sod3 mRNA levels were detected using quantitative polymerase chain reaction (Q-PCR). Oocyte quality was evaluated using in vitro fertilization (IVF) and zygote culture. Results: Consecutive superovulation groups presented lower P, E2, SOD, GSH-Px and INH B levels, significantly higher FSH, LH, MDA and ROS levels, and significantly fewer primordial follicles compared with the control group. Consecutive superovulation groups presented significantly increased levels of Sod2, 8-OhdG, 4-HNE, NTY, significantly increased levels of the SIRT1 and FOXO1 proteins, significantly increased levels of the senescence-associated protein p16, as well as decreased AMH, Sod1 and Sod3 levels and increased granulosa cell apoptosis compared with the control group. Conclusion: Consecutive superovulation significantly decreased ovarian function and oocyte quality and increased oxidative stress and apoptosis in the ovary via a mechanism involving the p16 and SIRT1/FOXO1 signaling pathways. These findings suggest that consecutive superovulation may be used to establish a mouse model of ovarian aging.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Establishment of a Mouse Model of Premature Ovarian Failure Using Consecutive Superovulation

Nie

Dai

Zheng

et al. 2018

Cell Physiol Biochem

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“…Others report moderate to high repeatability or correlations in number of antral follicles at emergence of two consecutive follicular waves (Singh et al, 2004) or following ultrasound-guided needle aspiration of antral follicles at various intervals in individual cattle (Boni et al, 1997), and from one menstrual cycle to the next in human beings (Scheffer et al, 1999). In addition, repeatability of follicle numbers during waves remains very high in individual cattle for at least 1 year (Burns et al, 2005), and others report numbers of growing preantral and antral follicles remain relatively constant until cattle are 7-9 years old (Erickson, 1966a;Katska and Smorag, 1984) and human beings are 35-40 years old (Gougeon et al, 1994;Scheffer et al, 1999;Tufan et al, 2004). Taken together, these observations imply that numbers of antral follicles during waves may remain relatively constant during extended periods of ovarian aging in individuals, despite significant loss of primordial follicles in the ovarian reserve.…”

Section: Discussionmentioning

confidence: 99%

Follicle numbers are highly repeatable within individual animals but are inversely correlated with FSH concentrations and the proportion of good-quality embryos after ovarian stimulation in cattle

et al. 2007

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BACKGROUND: The significance of the high variation in numbers of follicles produced during reproductive cycles in humans and cattle is unknown. METHODS: We selected beef heifers with high (25) or low (15) numbers of ovarian follicles and determined the association with alterations in FSH and estradiol concentrations, as well as responsiveness to superstimulation and embryo quality. The variation in follicle numbers was also compared with oocyte quality in natural cycles using IVF and abattoir sourced bovine ovaries. RESULTS: Results show that: (i) FSH was lower (P < 0.03) in animals with high compared with low follicle numbers per follicle wave; (ii) after superovulation, in the high versus low follicle number group, the number of oocytes/embryos recovered after insemination (10.6 + + + + + 2.7 versus 4.7 + + + + + 0.7) and the number of transferable embryos (5.4 + + + + + 1.3 versus 3.8 + + + + + 0.8) per animal were greater (P < 0.05), whereas the proportion of transferable embryos (50.7% versus 79.8%) was lower (P < 0.05); (iii) in unstimulated animals, the numbers of high-quality oocytes harvested and in-vitro fertilized oocytes developing into blastocysts were up to 4-fold greater (P < 0.05) for ovaries with high versus low numbers of follicles, but the proportions of oocytes developing into blastocysts were similar in the two groups. CONCLUSION: Phenotypic classification based on numbers of follicles may be useful to improve superovulation procedures. The lower proportion of transferable embryos following superovulation of ovaries with high numbers of follicles is probably not the result of differences in the quality of oocytes before superovulation.

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“…Female aging has many effects on endocrine parameters and body composition caused by a gradual depletion of the primordial follicle pool (2)(3)(4)(5). The diminishing pool of growing antral follicles results in decreasing serum levels of anti-Mullerian hormone (AMH), inhibin B, and E 2 .…”

mentioning

confidence: 99%

The phenotype of polycystic ovary syndrome ameliorates with aging

Brown¹,

Louwers²,

Fong³

et al. 2011

Fertility and Sterility

111

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Assessment of reproductive ageing patterns by hormonal and ultrasonographic ovarian reserve tests

Cited by 32 publications

References 28 publications

Establishment of a Mouse Model of Premature Ovarian Failure Using Consecutive Superovulation

Establishment of a Mouse Model of Premature Ovarian Failure Using Consecutive Superovulation

Follicle numbers are highly repeatable within individual animals but are inversely correlated with FSH concentrations and the proportion of good-quality embryos after ovarian stimulation in cattle

The phenotype of polycystic ovary syndrome ameliorates with aging

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