Assessment of rumen microbial diversity of buffalo raised under typical feeding condition using Illumina Sequencing technique

Natsir, Asmuddin; Nadir, Marhamah; Syahrir, Syahriani; Mujnisa, A.

doi:10.1088/1755-1315/247/1/012064

Cited by 4 publications

(1 citation statement)

References 20 publications

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“…Cellulase enzyme activity tests (CMCase, Avicelase and FPase) in this study were carried out at 39ºC and pH 6.8 to describe the activity of cellulase enzymes produced by anaerobic fungi in the rumen. Natsir et al (2019) stated that the optimum pH condition for growth microbial and fermentation process in rumen was 6.8. The research conducted by Setyoko and Utami (2016) also showed that the optimum temperature for cellulase enzyme activity isolated from rumen fluid was 39ºC.…”

Section: Cellulase Enzyme Activity In Anaerobic Fungi Isolated From B...mentioning

confidence: 99%

Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics

et al. 2022

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Abstract. Agustina S, Wiryawan KG, Suharti S, Meryandini A. 2022. Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics. Biodiversitas 23: 6448-6455. Anaerobic fungi are essential microbes in the degradation process of feed fiber in the rumen with the ability to produce fibrolytic enzymes and rhizoids that can penetrate feed particles. The activity of cellulase enzymes in fungi is influenced by several factors such as the type of feed and livestock used as a source of isolate. Therefore, this research aimed to analyze the types of anaerobic fungi isolated from buffalo based on their DNA nitrogenous bases composition and also to evaluate the growth rate, cellulase enzyme activity, and fermentation product concentration of anaerobic fungi. The growth rate of fungi was examined by measuring the biomass formed, while cellulase enzyme activity was carried out using CMC, Avicel, and Filter Paper as substrates. The fungal fermentation products were analyzed using the HPLC and GC methods. The results showed that the fungi isolated from buffalo rumen were closely related to anaerobic fungi type Piromyces sp., Caecomyces sp., and Neocallimastix frontalis, with different growth rate, cellulase enzyme activity, and the fermentation products concentration in each type of anaerobic fungi. Therefore, it can be concluded that anaerobic fungi isolated from buffalo rumen can degrade cellulose. It was also discovered that fungi Neocallimastix frontalis had a higher growth rate, cellulase enzyme activity (CMCase, avicelase, and FPase), and fermentation products than Piromyces sp. and Caecomyces sp.

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Section: Cellulase Enzyme Activity In Anaerobic Fungi Isolated From B...mentioning

confidence: 99%

Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics

et al. 2022

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Wheat cell walls and constituent polysaccharides induce similar microbiota profiles uponin vitrofermentation despite different short chain fatty acid end-product levels

et al. 2021

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Biostarter formulated from the rumen bacterial of buffalo effectively change the chemical composition of the rice straw

Natsir

Syahrir

Nadir

et al. 2020

IOP Conf. Ser.: Earth Environ. Sci.

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The use of such agricultural by-products as rice straw is important in fulfilling the needs of feed for ruminants, especially during the dry season. However, the main problem of using rice straw a feedstuff is low digestibility due to the existence of the links between lignin and cellulose or hemicellulose. This study was designed to evaluate the effects of the application of biostarter (BS) developed from rumen bacterial of buffalo on the of the changes of the chemical composition of the rice straw under a different duration of fermentation time. The experiment was carried out in a factorial arrangement according to a completely randomized design. The first factor was different levels of BS application, i.e. rice straw without BS (a1), rice straw + 5% BS (a2), rice straw +10% BS (a3), and rice straw + 15% BS (a4). The second factor was the duration of the fermentation time, i.e., two weeks (b1) and four weeks (b2). Each treatment combination was repeated three times, giving the total number of the experimental unit was 24. The results of the study indicated that increased levels of BS had a significant (P<0.05) effect on the concentration of crude protein, crude fiber, ash, and nitrogen-free extract (NFE) of the rice straw but no effects (P>0.05) on lipid content. The length of the fermentation period only affected (P<0.05) the crude fiber and crude protein content but no effects (P>0.05) on NFE, lipid, and ash. There was no interaction between levels of BS and the duration of the fermentation time on the chemical components of the rice straw. In conclusion, the use of BS was successfully changing the chemical composition of rice straw.

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Assessment of rumen microbial diversity of buffalo raised under typical feeding condition using Illumina Sequencing technique

Cited by 4 publications

References 20 publications

Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics

Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics

Wheat cell walls and constituent polysaccharides induce similar microbiota profiles uponin vitrofermentation despite different short chain fatty acid end-product levels

Biostarter formulated from the rumen bacterial of buffalo effectively change the chemical composition of the rice straw

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