Assessment of satellite cell number and activity status in human skeletal muscle biopsies

Mackey, Abigail L.; Kjær, Michael; Charifi, Nadia; Henriksson, Jan; Bojsen‐Møller, Jens; Holm, Lars; Kadi, Fawzi

doi:10.1002/mus.21369

Cited by 142 publications

(165 citation statements)

References 35 publications

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“…Upon serum withdrawal, CD56 + /desmin + cells readily completed terminal differentiation to form large multinucleated myotubes expressing MHC (Fig. 2M), confirming CD56 as a useful marker for human primary myogenic cells as suggested by others (Cashman et al, 1987;Moore et al, 1987;Schubert et al, 1989;Illa et al, 1992;Mackey et al, 2009). By contrast, the CD56 2 cells obtained from sorting expressed the fibroblast specific antigen TE-7 in culture and were incapable of myogenic differentiation (Fig.…”

Section: Cd56supporting

confidence: 69%

“…To achieve this we optimised a double immunomagnetic cell-sorting method based on the cell-surface expression of CD56 on myogenic cells (Cashman et al, 1987;Mackey et al, 2009) for the reproducible purification and subsequent culture of the major cell populations from human muscle. CD56 2 cells were confirmed as fibroblasts by their TE-7 expression.…”

Section: Introductionmentioning

confidence: 99%

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Human skeletal muscle fibroblasts, but not myogenic cells, readily undergo adipogenic differentiation

Agley

Rowlerson

Velloso

et al. 2013

Journal of Cell Science

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SummaryWe characterised the adherent cell types isolated from human skeletal muscle by enzymatic digestion, and demonstrated that even at 72 hours after isolation these cultures consisted predominantly of myogenic cells (CD56 2 fraction obtained from the first sort was highly enriched in TE-7 + fibroblasts. Using quantitative analysis of immunofluorescent staining for lipid content, lineage markers and transcription factors, we tested if the purified cell populations could differentiate into adipocytes in response to treatment with either fatty acids or adipocyte-inducing medium. Both treatments caused the fibroblasts to differentiate into adipocytes, as shown by loss of intracellular TE-7, upregulation of the adipogenic transcription factors PPARc and C/EBPa, and adoption of a lipid-laden adipocyte morphology. By contrast, myogenic cells did not undergo adipogenesis and showed differential regulation of PPARc and C/EBPa in response to these adipogenic treatments. Our results show that human skeletal muscle fibroblasts are at least bipotent progenitors that can remain as extracellular-matrix-producing cells or differentiate into adipocytes.

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Section: Cd56supporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Human skeletal muscle fibroblasts, but not myogenic cells, readily undergo adipogenic differentiation

Agley

Rowlerson

Velloso

et al. 2013

Journal of Cell Science

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“…In adults, the percentage of satellite cells in soleus muscle is generally two-to fourfold higher than that in tibialis anterior muscle or EDL muscle (190,466,500). Within the same muscle, the number of satellite cells found on slow muscle myofibers (type I) is generally higher than those on fast myofibers (type IIa and type IIb) (190,324,383). The biological meaning and the potential regulatory mechanisms underlying these phenomena are poorly understood.…”

Section: Variance Of Satellite Cells Number and Locationmentioning

confidence: 99%

Satellite Cells and the Muscle Stem Cell Niche

Yin

Price

Rudnicki

2013

Physiological Reviews

1,763

1,808

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LYin H, Price F, Rudnicki MA. Satellite Cells and the Muscle Stem Cell Niche. Physiol Rev 93: 23-67, 2013; doi:10.1152/physrev.00043.2011.-Adult skeletal muscle in mammals is a stable tissue under normal circumstances but has remarkable ability to repair after injury. Skeletal muscle regeneration is a highly orchestrated process involving the activation of various cellular and molecular responses. As skeletal muscle stem cells, satellite cells play an indispensible role in this process. The self-renewing proliferation of satellite cells not only maintains the stem cell population but also provides numerous myogenic cells, which proliferate, differentiate, fuse, and lead to new myofiber formation and reconstitution of a functional contractile apparatus. The complex behavior of satellite cells during skeletal muscle regeneration is tightly regulated through the dynamic interplay between intrinsic factors within satellite cells and extrinsic factors constituting the muscle stem cell niche/microenvironment. For the last half century, the advance of molecular biology, cell biology, and genetics has greatly improved our understanding of skeletal muscle biology. Here, we review some recent advances, with focuses on functions of satellite cells and their niche during the process of skeletal muscle regeneration.

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“…The magnitude of increase in young men (3.2±1.3 fold) was higher than in older participants (1.3±0.6 fold) when expressed relative to pre-exercise numbers 88) . Electrical stimulation of the gastrocnemius muscle also increased the number of activated satellite cells (+CD56+Ki67) within 48 hours in young (20±5 years) subjects 90) . Combined exercise with cycle ergometer ( < 62% of workload capacity) and leg press machine (55-70% 1RM) activated satellite cells (+DLK1+CD56) within 9 hours in young subjects 91) .…”

Section: Effect Of Resistance Training On Satellite Cell Numbermentioning

confidence: 89%

Roles played by protein metabolism and myogenic progenitor cells in exercise-induced muscle hypertrophy and their relation to resistance training regimens

Ishii

Ogasawara

Kobayashi

et al. 2012

JPFSM

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To learn the mechanisms underlying resistance exercise-induced muscle hypertrophy, recent studies on muscle protein metabolism and myogenic progenitor cells were reviewed. Numerous studies have suggested that activation of the translation process plays a major role in a resistance exercise-induced increase in muscle protein synthesis, and also in muscle hypertrophy after a prolonged period of training. Among regulators of the translational activity, the mTORC1 signaling pathway has been shown to be important, although the relation between its upstream regulation and exercise regimen remains unclear. In addition, the muscle satellite cells play a part, even if not indispensable, in exercise-induced muscle hypertrophy, by supplying muscle fibers with new myonuclei. Middle to high exercise intensity has been regarded as essential for gaining muscle mass, because it causes the recruitment of large motor units with fast, type II muscle fibers, which are readily hypertrophied through activation of mTORC1 signaling. However, several studies have shown that low-intensity resistance exercises with either large exercise volume or prolonged contraction time effectively activate protein synthesis and induce muscle hypertrophy. These findings suggest that various strategies are possible in exercise regimens, and exercise intensity is not necessarily a primary factor for gaining muscular size.

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Assessment of satellite cell number and activity status in human skeletal muscle biopsies

Cited by 142 publications

References 35 publications

Human skeletal muscle fibroblasts, but not myogenic cells, readily undergo adipogenic differentiation

Human skeletal muscle fibroblasts, but not myogenic cells, readily undergo adipogenic differentiation

Satellite Cells and the Muscle Stem Cell Niche

Roles played by protein metabolism and myogenic progenitor cells in exercise-induced muscle hypertrophy and their relation to resistance training regimens

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