Assessment of <scp>BRAF</scp> V600E (<scp>VE</scp>1) protein expression and <i>BRAF</i> gene mutation status in codon 600 in benign and malignant salivary gland neoplasms

Bodnar, Magdalena; Burduk, Paweł; Antosik, Paulina; Jarmuż-Szymczak, Małgorzata; Wierzbicka, Małgorzata; Marszałek, Andrzej

doi:10.1111/jop.12504

Cited by 8 publications

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“…Immunohistochemical staining was carried out as described elsewhere [ 14 – 16 ], and the results were standardized against a series of positive and negative controls. Positive control staining was performed on a model tissue section selected according to The Human Protein Atlas ( http://www.proteinatlas.org ) [ 17 ] and antibody specification (Additional file 1 : Table S1) and the antibody datasheet.…”

Section: Methodsmentioning

confidence: 99%

“…Each slide was examined under ECLIPSE E400 light microscope (Nikon Instruments Europe, Amsterdam, Netherlands) with the low-power (20×) objective. The result of immunohistochemical staining was expressed according to Immunoreactive Remmele-Stegner (IRS) score [ 18 ], described in detail in our previous papers [ 14 – 16 , 19 ]. Total IRC score (from 0 to 12) was obtained by multiplying the staining intensity score (0—negative, 1—weak staining, 2—moderate staining, 3—strong staining) by the relative proportion of immunolabeled specimen area (0—none; 1—less than 10%; 2—10 to 50%; 3—50 to 80%; 4—at least 80%).…”

Section: Methodsmentioning

confidence: 99%

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Characteristic profiles of DNA epigenetic modifications in colon cancer and its predisposing conditions—benign adenomas and inflammatory bowel disease

et al. 2018

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BackgroundActive demethylation of 5-methyl-2′-deoxycytidine (5-mdC) in DNA occurs by oxidation to 5-(hydroxymethyl)-2′-deoxycytidine (5-hmdC) and further oxidation to 5-formyl-2′-deoxycytidine (5-fdC) and 5-carboxy-2′-deoxycytidine (5-cadC), and is carried out by enzymes of the ten-eleven translocation family (TETs 1, 2, 3). Decreased level of epigenetic DNA modifications in cancer tissue may be a consequence of reduced activity/expression of TET proteins. To determine the role of epigenetic DNA modifications in colon cancer development, we analyzed their levels in normal colon and various colonic pathologies. Moreover, we determined the expressions of TETs at mRNA and protein level.The study included material from patients with inflammatory bowel disease (IBD), benign polyps (AD), and colorectal cancer (CRC). The levels of epigenetic DNA modifications and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) in examined tissues were determined by means of isotope-dilution automated online two-dimensional ultraperformance liquid chromatography with tandem mass spectrometry (2D-UPLC-MS/MS). The expressions of TET mRNA were measured with RT-qPCR, and the expressions of TET proteins were determined immunohistochemically.ResultsIBD was characterized by the highest level of 8-oxodG among all analyzed tissues, as well as by a decrease in 5-hmdC and 5-mdC levels (at a midrange between normal colon and CRC). AD had the lowest levels of 5-hmdC and 5-mdC of all examined tissues and showed an increase in 8-oxodG and 5-(hydroxymethyl)-2′-deoxyuridine (5-hmdU) levels. CRC was characterized by lower levels of 5-hmdC and 5-mdC, the lowest level of 5-fdC among all analyzed tissues, and relatively high content of 5-cadC. The expression of TET1 mRNA in CRC and AD was significantly weaker than in IBD and normal colon. Furthermore, CRC and AD showed significantly lower levels of TET2 and AID mRNA than normal colonic tissue.ConclusionsOur findings suggest that a complex relationship between aberrant pattern of DNA epigenetic modification and cancer development does not depend solely on the transcriptional status of TET proteins, but also on the characteristics of premalignant/malignant cells. This study showed for the first time that the examined colonic pathologies had their unique epigenetic marks, distinguishing them from each other, as well as from normal colonic tissue. A decrease in 5-fdC level may be a characteristic feature of largely undifferentiated cancer cells.Electronic supplementary materialThe online version of this article (10.1186/s13148-018-0505-0) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Characteristic profiles of DNA epigenetic modifications in colon cancer and its predisposing conditions—benign adenomas and inflammatory bowel disease

et al. 2018

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“…Bodnar et al showed that some salivary gland neoplasms had either nuclear or cytoplasmic expression of BRAF V600E protein on IHC analysis. However, their polymerase chain reaction‐based studies did not detect a corresponding mutation 11 …”

Section: Discussionmentioning

confidence: 96%

Metastatic malignant melanoma mimicking a salivary gland basaloid neoplasm after treatment with nivolumab

Lajara

Landau

2021

Diagnostic Cytopathology

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Malignant melanoma is a well‐known diagnostic pitfall, given its propensity to metastasize to different sites and mimic various entities. In this report, we present a fine‐needle aspiration biopsy (FNA) of a metastatic melanoma with basaloid features that is occurring in the preauricular/parotid area. The patient is a 17‐year‐old male with a history of excision of melanoma of the left temple, and was undergoing adjuvant treatment with nivolumab. The prior excision was positive for S100, HMB‐45, melan‐A, and tyrosinase. On follow‐up, he presented with non‐FDG avid left preauricular area lesions. FNA was performed, and on‐site evaluation demonstrated a cellular basaloid neoplasm with focal fibrillary stroma. Immunohistochemical stains revealed that the tumor cells were positive for SOX‐10, S100, MITF, and HMGA2, and were negative for HMB‐45, melan‐A, tyrosinase, p63, cam 5.2 and PLAG1. The positive S100, SOX‐10, and MITF results and negative cam 5.2 result supported the diagnosis of melanoma. Nivolumab was then stopped, Dabrafenib/Trametinib were started, and the patient underwent excision of the nodules. Nine‐months later, he developed a rib metastasis that was positive for S100, SOX‐10, melan‐A, and tyrosinase. This report emphasizes that melanoma involving the parotid gland region has the potential to be misdiagnosed by FNA as a salivary gland neoplasm because of overlapping cytologic features and immunophenotypes. This pitfall is avoided by careful morphologic analysis and judicious use of ancillary studies.

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“…The immunohistochemical staining was performed according to previously described procedures [34, 35] and standardized using a series of positive and negative control reactions. The positive control reaction was performed on a model tissue selected according to reference sources (The Human Protein Atlas, http://www.proteinatlas.org) [36] and the antibody datasheets.…”

Section: Methodsmentioning

confidence: 99%

Relationship between Helicobacter pylori Infection and Plasmacytoid and Myeloid Dendritic Cells in Peripheral Blood and Gastric Mucosa of Children

Helmin-Basa

Wiese-Szadkowska

Szaflarska-Popławska

et al. 2019

Mediators of Inflammation

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Purpose To investigate the frequency and activation status of peripheral plasmacytoid DCs (pDCs) and myeloid DCs (mDCs) as well as gastric mucosa DC subset distribution in Helicobacter pylori- (H. pylori-) infected and noninfected children. Materials and Methods Thirty-six children were studied; twenty-one had H. pylori. The frequencies of circulating pDCs (lineage−HLA-DR+CD123+) and mDCs (lineage−HLA-DR+CD11c+) and their activation status (CD83, CD86, and HLA-DR expression) were assessed by flow cytometry. Additionally, the densities of CD11c+, CD123+, CD83+, CD86+, and LAMP3+ cells in the gastric mucosa were determined by immunohistochemistry. Results The frequency of circulating CD83+ mDCs was higher in H. pylori-infected children than in the noninfected controls. The pDCs demonstrated upregulated HLA-DR surface expression, but no change in CD86 expression. Additionally, the densities of gastric lamina propria CD11c+ cells and epithelial pDCs were increased. There was a significant association between frequency of circulating CD83+ mDCs and gastric lamina propria mDC infiltration. Conclusion This study shows that although H. pylori-infected children had an increased population of mature mDCs bearing CD83 in the peripheral blood, they lack mature CD83+ mDCs in the gastric mucosa, which may promote tolerance to local antigens rather than immunity. In addition, this may reduce excessive inflammatory activity as reported for children compared to adults.

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Assessment of BRAF V600E (VE1) protein expression and BRAF gene mutation status in codon 600 in benign and malignant salivary gland neoplasms

Abstract: In studied salivary gland cancers, no PCR-based prove mutations of BRAF V600 were detected. Further molecular analyses are necessary to rapid molecular arrays for the identification of specific mutations, optimal for individualized targeted therapies.

Cited by 8 publications

References 31 publications

Characteristic profiles of DNA epigenetic modifications in colon cancer and its predisposing conditions—benign adenomas and inflammatory bowel disease

Characteristic profiles of DNA epigenetic modifications in colon cancer and its predisposing conditions—benign adenomas and inflammatory bowel disease

Metastatic malignant melanoma mimicking a salivary gland basaloid neoplasm after treatment with nivolumab

Relationship between Helicobacter pylori Infection and Plasmacytoid and Myeloid Dendritic Cells in Peripheral Blood and Gastric Mucosa of Children

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