Assessment of sexual maturity in captive Amazonian manatees (<i>Trichechus inunguis</i>)

Amaral, Rodrigo de Souza; Silva, Vera Maria Ferreira da; Lazzarini, Stella Maris; Neto, José Anselmo d’Affonseca; Ribeiro, Daniella Carvalho; Rosas, Fernando C. W.

doi:10.1111/mms.12439

Cited by 9 publications

(6 citation statements)

References 37 publications

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“…Owing to the large sample size and temporal scale, three‐way ANOVAs were fitted to determine the effects of time, sex and ontogenetic class on δ 13 C and δ 15 N values for animals from the várzeas . Manatees were classified as adults based on age at sexual maturity and body length following Amaral et al (2017): ≥9 years old and ≥200 cm. The age of most individuals was previously determined by Vergara‐Parente (2009) through growth‐layer‐group counts in periotic bone according to Marmontel et al (1996).…”

Section: Methodsmentioning

confidence: 99%

“…The analytical precision of the laboratory standards was <0.04‰ for δ 13 C and <0.2‰ for δ 15 N. 2009) through growth-layer-group counts in periotic bone according to Marmontel et al (1996). Samples of unknown age were classified based only on body length (Amaral et al, 2017). Individuals were discarded from analyses when age and length did not agree.…”

Section: Stable Isotope Analysismentioning

confidence: 99%

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Spatio‐temporal, ontogenetic and sex‐related patterns in resource use of Amazonian manatees across floodplains and estuaries as inferred by δ¹³C and δ¹⁵N isotopic values

Carvalho

Marmontel

Botta

et al. 2022

Aquatic Conservation

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1. Threats to aquatic mammals may occur in isolation or in combination, leading to cumulative effects on populations and their ecosystems. Thus, conservation actions must consider the complexity of factors involved in patterns of habitat use, including feeding ecology, such as ontogenetic, spatial and temporal dietary shifts.2. The Amazonian manatee (Trichechus inunguis) occurs in a variety of environments, such as várzeas and igap os (flooded areas) and estuaries. Different environments can lead to variation in the diet of manatees according to the availability and abundance of plant species.3. Stable isotope analysis (δ 13 C and δ 15 N) of manatee teeth was performed to assess spatio-temporal, ontogenetic and sex-related patterns in resource use. Mixing models were applied to identify the most consumed plant groups.4. In várzeas, there were significant interactions between sex and ontogenetic class and ontogenetic class and time series for δ 13 C values. For manatees sampled from 1994 to 2004, males and adults showed the highest δ 15 N values, and aquatic C 3 , followed by C 4 plants, were the groups that most contributed to the species' diet. 5. Comparisons among the environments showed the highest δ 13 C values for manatees from várzeas and the lowest values for manatees from igap os. Várzeas

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Section: Methodsmentioning

confidence: 99%

Section: Stable Isotope Analysismentioning

confidence: 99%

Spatio‐temporal, ontogenetic and sex‐related patterns in resource use of Amazonian manatees across floodplains and estuaries as inferred by δ¹³C and δ¹⁵N isotopic values

Carvalho

Marmontel

Botta

et al. 2022

Aquatic Conservation

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“…Blood samples were centrifuged and serum was stored at −20°C until hormonal analysis. Serum progesterone and oestradiol levels were evaluated using an enzyme immunoassay protocol previously validated for different animal species (Munro et al , 1991; Graham et al , 2001) and modified by Amaral et al (2018). The CL425 and R0008 antibodies, for progesterone and oestradiol, respectively, and their respective conjugated hormones, were provided by the University of California, Davis (USA).…”

Section: Methodsmentioning

confidence: 99%

First report on the colpocytology and serum steroid hormone levels of Giant otters Pteronura brasiliensis

Amaral

Rosas

2020

International Zoo Yearbook

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Considering the importance of tools for the reproductive monitoring and management of Giant otters Pteronura brasiliensis in the wild or in captivity, this study reports the relationship among the colpocytology, reproductive serum hormone (progesterone and oestradiol) levels and ovarian morphology of adult Giant otters. Blood and vaginal cytology samples were obtained from four adult females (two free-ranging and two in human care). The ovaries were collected from the captive females. The data were correlated to define the phase of the oestrous cycle of each animal. The results suggest the feasibility of using hormone analysis or colpocytology to aid in the reproductive monitoring of Giant otters in human care. In addition, these tools could be used to define the reproductive status of individual wild Giant otters during capture procedures.

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“…For hormone extraction from serum samples, the protocol using diethyl ether as described by Rasmussen et al (16) was used, followed by resuspension in buffer solution. Samples extracted from serum and feces were analyzed by enzyme immunoassay using a protocol described for several other species (17)(18)(19) . Antibodies CL425 (1: 5,000) for progesterone and R522-2 (1: 20,000) for total estrone, with their respective peroxidase-conjugated hormones (1: 160,000; 1: 350,000), all provided by the University of Davis -UC Davis, USA, were used.…”

Section: Materials E Methodsmentioning

confidence: 99%

Monitoring of Progesterone and Estrone Fecal Metabolites Throughout Gestation in Ewes

et al. 2019

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The aim of this study was to monitor the progesterone and fecal estrone metabolites throughout gestation in ewes correlating with the serum levels of these steroid hormones. Therefore, fecal and serum samples were collected from 5 weeks before mating and gestation until two weeks postparturition. Serum levels of progesterone and estrone and their fecal metabolites were measured by enzyme immunoassay. Serum and fecal hormonal patterns showed a significant correlation for both hormones (R = 0.8572, P < 0.001 for progesterone and R = 0.5893, P < 0.001 for estrone). The fecal progesterone metabolite levels showed significant increasing values among the three thirds of pregnancies, consistent with the serum levels and with the literature. Additionally, the prepartum peak of estrone in the fecal matrix was identified but without observation in the serum matrix due to the blood collection interval used. Therefore, this study demonstrated the viability of progesterone and estrone monitoring throughout gestation using fecal samples, making noninvasive longitudinal endocrine monitoring throughout gestation possible in this species. ResumoO objetivo deste estudo foi monitorar os níveis de metabólitos fecais de progesterona e estrona ao longo da gestação em ovelhas, correlacionando-os com os níveis séricos desses hormônios esteroides. Assim, amostras de fezes e sangue foram colhidas de cinco ovelhas no período pré-cobertura e durante a gestação, até duas semanas após o parto. Os níveis séricos de progesterona e estrona e de seus metabólitos fecais foram mensurados por enzimaimunoensaio. Os perfis hormonais séricos e fecais apresentaram correlação positiva significativa para os dois hormônios (R = 0,8572, P < 0,001 para progesterona e R = 0,5893, P < 0,001 para estrona). Os níveis de metabólitos fecais de progesterona apresentaram valores significativamente crescente entre os terços da gestação, corroborando com os níveis séricos e com os relatos da literatura. Adicionalmente, foi possível evidenciar o pico pré-parto de estrona na matriz fecal, porém sem registro na matriz sérica, provavelmente devido ao intervalo de coletas aplicado. Deste modo, este estudo demonstrou a AMARAL, R.S. et al.Cienc. anim. bras., Goiânia, v.20, 1-10, e-54208, 2019 viabilidade do monitoramento dos níveis de progesterona e estrona durante a gestação em ovinos utilizando amostras fecais, possibilitando monitoramento endócrino longitudinal não invasivo durante a gestação nessa espécie.

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Assessment of sexual maturity in captive Amazonian manatees (Trichechus inunguis)

Cited by 9 publications

References 37 publications

Spatio‐temporal, ontogenetic and sex‐related patterns in resource use of Amazonian manatees across floodplains and estuaries as inferred by δ¹³C and δ¹⁵N isotopic values

Spatio‐temporal, ontogenetic and sex‐related patterns in resource use of Amazonian manatees across floodplains and estuaries as inferred by δ¹³C and δ¹⁵N isotopic values

First report on the colpocytology and serum steroid hormone levels of Giant otters Pteronura brasiliensis

Monitoring of Progesterone and Estrone Fecal Metabolites Throughout Gestation in Ewes

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Assessment of sexual maturity in captive Amazonian manatees (Trichechus inunguis)

Cited by 9 publications

References 37 publications

Spatio‐temporal, ontogenetic and sex‐related patterns in resource use of Amazonian manatees across floodplains and estuaries as inferred by δ13C and δ15N isotopic values

Spatio‐temporal, ontogenetic and sex‐related patterns in resource use of Amazonian manatees across floodplains and estuaries as inferred by δ13C and δ15N isotopic values

First report on the colpocytology and serum steroid hormone levels of Giant otters Pteronura brasiliensis

Monitoring of Progesterone and Estrone Fecal Metabolites Throughout Gestation in Ewes

Contact Info

Product

Resources

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Spatio‐temporal, ontogenetic and sex‐related patterns in resource use of Amazonian manatees across floodplains and estuaries as inferred by δ¹³C and δ¹⁵N isotopic values

Spatio‐temporal, ontogenetic and sex‐related patterns in resource use of Amazonian manatees across floodplains and estuaries as inferred by δ¹³C and δ¹⁵N isotopic values