Assessment of skin barrier function and biochemical changes of ex vivo human skin in response to physical and chemical barrier disruption

Döge, Nadine; Avetisyan, Araks; Hadam, Sabrina; Pfannes, Eva K. B.; Rancan, Fiorenza; Blume-Peytavi, Ulrike; Vogt, Annika

doi:10.1016/j.ejpb.2016.12.012

Cited by 31 publications

(23 citation statements)

References 55 publications

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“…The IL-8 concentrations of all samples at t 0 h ranged below the detection limit, whereas the average IL-8 amounts at t 20 h had noticeably risen but showed no differences between infected and uninfected wounds ( Fig 6D ). Döge et al found similar IL-6 and IL-8 levels in excised, uninfected human skin with mild barrier disruption [ 24 ], which suggests that the IL-6 and IL-8 amounts in uninfected ex vivo samples have to be credited solely to the damaged tissue.…”

Section: Resultsmentioning

confidence: 99%

Development, standardization and testing of a bacterial wound infection model based on ex vivo human skin

et al. 2017

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Current research on wound infections is primarily conducted on animal models, which limits direct transferability of these studies to humans. Some of these limitations can be overcome by using–otherwise discarded—skin from cosmetic surgeries. Superficial wounds are induced in fresh ex vivo skin, followed by intradermal injection of Pseudomonas aeruginosa under the wound. Subsequently, the infected skin is incubated for 20 hours at 37°C and the CFU/wound are determined. Within 20 hours, the bacteria count increased from 107 to 109 bacteria per wound, while microscopy revealed a dense bacterial community in the collagen network of the upper wound layers as well as numerous bacteria scattered in the dermis. At the same time, IL-1alpha and IL-1beta amounts increased in all infected wounds, while—due to bacteria-induced cell lysis—the IL-6 and IL-8 concentrations rose only in the uninfected samples. High-dosage ciprofloxacin treatment resulted in a decisive decrease in bacteria, but consistently failed to eradicate all bacteria. The main benefits of the ex vivo wound model are the use of healthy human skin, a quantifiable bacterial infection, a measureable donor-dependent immune response and a good repeatability of the results. These properties turn the ex vivo wound model into a valuable tool to examine the mechanisms of host-pathogen interactions and to test antimicrobial agents.

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Section: Resultsmentioning

confidence: 99%

Development, standardization and testing of a bacterial wound infection model based on ex vivo human skin

et al. 2017

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“…Covering a search period until May 2012, TEWL reference values for 50 skin areas in young and aged healthy humans were summarized . These reference values proved to be useful for designing clinical and experimental studies and for interpreting study results …”

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confidence: 99%

Transepidermal water loss in healthy adults: a systematic review and meta-analysis update

et al. 2018

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Reference estimates are useful for clinical study planning and interpretation of results. TEWL is highly dependent on skin area, and our results further support the symmetry between right and left measuring sites. TEWL in elderly people seems to be generally similar or decreased compared with younger individuals, but available evidence is limited. Reporting of TEWL should be improved: mean and spread parameters should always be reported in future studies.

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“…Quantification of protein extracted from tapes is frequently used for the investigation of interleukins and cytokines in healthy and diseased skin and scalp [6,11,12,13,14]. Because CSSS removes not only skin surface material, but approximately 30% of the stratum corneum [10], biomarkers extracted from such material may yield more relevant information compared to conventional adhesive tape strips.…”

Section: Introductionmentioning

confidence: 99%

Physiological and Molecular Effects of in vivo and ex vivo Mild Skin Barrier Disruption

Pfannes

Weiss²,

Hadam³

et al. 2018

Skin Pharmacol Physiol

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The success of topically applied treatments on skin relies on the efficacy of skin penetration. In order to increase particle or product penetration, mild skin barrier disruption methods can be used. We previously described cyanoacrylate skin surface stripping as an efficient method to open hair follicles, enhance particle penetration, and activate Langerhans cells. We conducted ex vivo and in vivo measurements on human skin to characterize the biological effect and quantify barrier disruption-related inflammation on a molecular level. Despite the known immunostimulatory effects, this barrier disruption and hair follicle opening method was well accepted and did not result in lasting changes of skin physiological parameters, cytokine production, or clinical side effects. Only in ex vivo human skin did we find a discrete increase in IP-10, TGF-β, IL-8, and GM-CSF mRNA. The data underline the safety profile of this method and demonstrate that the procedure per se does not cause substantial inflammation or skin damage, which is also of interest when applied to non-invasive sampling of biomarkers in clinical trials.

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Assessment of skin barrier function and biochemical changes of ex vivo human skin in response to physical and chemical barrier disruption

Cited by 31 publications

References 55 publications

Development, standardization and testing of a bacterial wound infection model based on ex vivo human skin

Development, standardization and testing of a bacterial wound infection model based on ex vivo human skin

Transepidermal water loss in healthy adults: a systematic review and meta-analysis update

Physiological and Molecular Effects of in vivo and ex vivo Mild Skin Barrier Disruption

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