Assessment of somaclonal variation in somatic embryo-derived plants of yacon [Smallanthus sonchifolius (Poepp. and Endl.) H. Robinson] using inter simple sequence repeat analysis and flow cytometry

Viehmannová, Iva; Bortlova, Zuzana; Vítámvás, Jan; Čepková, Petra Hlásná; Eliášová, Kateřina; Svobodová, Eva; Trávníčková, Martina

doi:10.1016/j.ejbt.2013.12.011

Cited by 28 publications

(14 citation statements)

References 32 publications

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“…Frequency of SV in tissue culture derived plantlets depends on genotype, explant type, culture environment, exogenous PGR, mode of regeneration and culture age (Table 2) (Hitomi et al 1998;Etienne and Bertrand 2003;Rodríguez López et al 2010b;Krishna et al 2016). The main SV shown in tissue culture are variation in the number and structure of the chromosomes, DNA sequence variation, mobile element activation, chromatin remodeling and DNA methylation (Ruiz et al 1992;Viehmannova et al 2014). The first report of SV was made by Heinz and Mee (1971) in sugarcane (Heinz and Mee 1971), and after that many reports followed in different species (reviewed in (Krishna et al 2016)).…”

Section: Abnormalities Related To Sv In Somatic Embryogenesismentioning

confidence: 99%

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Garcia¹,

Almeida

Costa

et al. 2019

Plant Cell Tiss Organ Cult

110

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Somatic embryogenesis is a morphogenetic event where somatic cells have the ability to produce embryos without gamete fusion. It is used as a technique for plant mass propagation. It is a process that has six well defined steps such as induction, expression, development, maturation, germination and plant conversion. These steps are characterized by distinct physiological, morphological and molecular events. Although somatic embryogenesis has been established in several plant species, there remains many problems to be solved. The main problem in somatic embryogenesis is the large number of abnormal embryos produced which cannot germinate nor convert into normal plants. Abnormalities in somatic embryos (SE) can be generated by genetic or epigenetic changes in the DNA. These changes in the DNA can be influenced by external factors such as the use of plant growth regulators and mutagenic substances or stress factors applied to the plant tissue such as high and low temperatures, drought, salinity, and heavy metals. Abnormalities generated by genetic changes in the DNA are hardly reversible; however, abnormalities generated by epigenetic changes may be reversible and the abnormal embryos are able to produce normal plants in most cases. This review focuses on the identification of the main factors that can cause abnormal SE development in different plant species, suggest how SE abnormalities are related to somaclonal variations and identify which genes may be involved with embryo abnormalities. Zygotic embryo abnormalities in Arabidopsis thaliana mutants are listed with the aim to understand the main genetic mechanisms involved in embryo aberrations. Key messageThe abnormalities in somatic embryos are related to the use of 2,4-D in most of the published protocols, this sintetic auxin disrupts the endogenous auxin balance and the auxin polar transportation interfering with the embryo apical-basal polarity.

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Section: Abnormalities Related To Sv In Somatic Embryogenesismentioning

confidence: 99%

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Garcia¹,

Almeida

Costa

et al. 2019

Plant Cell Tiss Organ Cult

110

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“…The studies by Viehmannova et al [2014], using ISSR markers and flow cytometry in yacon on somatic embryo-derived plants also showed polymorphisms evidence of somaclonal variation, however a cytometric study showed no differences. No differences in ploidy, which could be detected by FCM, demonstrates that during SE, the number of chromosomes or the ploidy of the test plants does not change.…”

Section: Resultsmentioning

confidence: 94%

Genetic Diversity of Chrysanthemum Plants Derived via Somatic Embryogenesis Using Rapd Markers

Lema-Rumińska¹,

Mellem²

2017

Acta Sci. Pol. Hortorum Cultus

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The genetic diversity was investigated among two chrysanthemum (Chrysanthemum × grandiflorum Ramat./Kitam.) cultivars 'Lady Salmon' and 'Lady Vitroflora' and its 15 lines of plants derived from somatic embryos in using ten random amplified polymorphic DNA (RAPD) markers. All primers gave 108 bands with 1218 products from 148.65 to 4391.20 bp in size. The average number of bands per primer was 10.8. Most fragments (54; 50%) were polymorphic, 9 (0.8%) specific and others (45; 49.2%) were monomorphic. Cluster analysis grouped all the cultivars and their lines into two main clusters and two subclusters. Most genetic diversity was characteristic for LS2 lines of plants derived via somatic embryogenesis from cultivar 'Lady Salmon'. All lines were different from each other and from the original cultivar propagated by meristematic explants. RAPD markers are a helpful tool to detect the genetic diversity of chrysanthemum plants derived via somatic embryogenesis (SE). Our result will provide useful information for production laboratory and for breeding programmes.

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“…and Endl.) H. Robinson было показано, что анализ относительного содержания ДНК не выявил значительных отклонений в размере генома регенерантов (Viehmannova et al, 2014). Регенеранты Coriandrum sativum L. полученные в результате соматического эмбриогенеза, также отличались размером генома схожим с образцами растений доноров (Ali et al, 2016).…”

Section: рис 1 типичные гистограммы характерные для диплоидных (а) unclassified

Уровни Плоидности И Относительного Содержания Днк В Культуре Клеток И Тканей Растений in Vitro

Skaptsov

Krasnoborodkina

Куцев

et al. 2016

Biol. Bull. of Bogdan Chmelnitskiy Melitopol St. Pedag. Univ.

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В данном исследовании представлены сведения об изменчивости в уровнях плоидности и размера генома регенерантов R. acetosa полученных методами не прямого и прямого морфогенеза в культуре in vitro. Культивирование мезофильных эксплантов проводили в присутствии ауксинов для поддержания пролиферации каллуса с последующем стимулированием непрямого морфогенеза. Для стимулирования прямого морфогенеза, апикальные меристемы проростков культивировали на питательных средах с преобладанием цитокининов. Исследование уровня плоидности и размера генома проводили методами проточной цитометрии и световой микроскопии. Нами установлено, что максимальные вариации в размере генома регенерантов отмечаются для растений, полученных через процесс непрямого морфогенеза. Также, в данном случае, были отмечены тетраплоидные формы с удвоенным содержанием ДНК. Altai State University, 656049, Barnaul, Lenina ave, mariakholodkova@gmail.com, m_kucev@mail.ru, serg_sm_@mail.ru, ssbgbot@mail.ru, amatsyura@gmail.com We presented results of variations in the ploidy level and the genome size of the R. acetosa regenerants. These regenerants was obtained by indirect and direct morphogenesis in in vitro culture. Explants were prepared from seedlings on the three-leaf stage of plant development. More than 100 explants were used to stimulate the indirect and direct morphogenesis. Mesophilic explants were cultured on the MS nutrient medium containing auxin to callus proliferation (2 mg/L NAA, 1 mg/L BA). Cultivation of the callus was maintained for 4 weeks followed by an indirect morphogenes. Indirect morphogenesis stimulated on the MS medium with cytokinin and gibberellic acid predominance (0.5 mg/L BA, 0.2 mg/L GA3). Direct stimulate morphogenesis from the apical meristem of seedlings on nutrient media with a predominance of cytokinins (1 mg/L BA, 0.25 mg/L NAA). Rhizogenesis have stimulated by transferring of the regenerants to the ½MS medium supplemented with 0.2 mg/L of NAA. Research of a ploidy level and genome size was performed by flow cytometry used propidium iodide staining with Vicia faba cv "Innovec" (2C=26.90 pg) as internal DNA standard. We calculated the relative DNA content (2C) for R. acetosa equal to 6,98 pg. Cytogenetical analisis showed that the maximum genome size variation recorded for regenerants obtained through the indirect morphogenesis. Variations in the genome size of the regenerants obtained by direct morphogenesis deviates from the control group to 0.30 pg (2С=7.28 pg) and after indirect morphogenesis to 1.04 pg (2С=8.2 pg). Cytogenetical analysis of the regenerated plants showed the presence of different somatic chromosome numbers ranging from 2n = 14 to 2n = 28. The relative DNA content of tetraploid forms was 11.87 pg. In our study was shown, that the most effective method of plant conservation in the in vitro culture is a direct morphogenesis. Analysis of the relative nuclear DNA content and chromosome counts of regenerants obtained by indirect morphogenesis from the callus cultures showed significant variations in the ...

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Assessment of somaclonal variation in somatic embryo-derived plants of yacon [Smallanthus sonchifolius (Poepp. and Endl.) H. Robinson] using inter simple sequence repeat analysis and flow cytometry

Cited by 28 publications

References 32 publications

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Abnormalities in somatic embryogenesis caused by 2,4-D: an overview

Genetic Diversity of Chrysanthemum Plants Derived via Somatic Embryogenesis Using Rapd Markers

Уровни Плоидности И Относительного Содержания Днк В Культуре Клеток И Тканей Растений in Vitro

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