Assessment of status of three water bodies in Serbia based on tissue metal and metalloid concentration (ICP-OES) and genotoxicity (comet assay)

Sunjog, Karolina; Kolarević, Stoimir; Kračun-Kolarević, Margareta; Višnjić-Jeftić, Željka; Skorić, Stefan; Gačić, Zoran; Lenhardt, Mirjana; Vasić, Nebojša; Vuković-Gačić, Branka

doi:10.1016/j.envpol.2016.03.008

Cited by 32 publications

(10 citation statements)

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“…The heavy metals and metalloids occur naturally in the environment. Earth's crust has these components and anthropogenic effects cause them to mix into sediments, soils and waters (Sunjog et al 2016). Many water sources were exposed to heavy metal and metalloid contamination from industrial, agricultural and human sources.…”

Section: Resultsmentioning

confidence: 99%

“…Besides, they are used in vivo, in vitro and in situ genotoxic monitoring of rivers and lakes. Fishes, such as Squalius cephalus (Sunjog et al 2016), Oreochromis niloticus (Hemachandra and Pathiratne 2016), Channa punctatus (Javed et al 2016), Abramis brama (Kostić et al 2016), Alburnus alburnus (Jovanović et al 2018), Labeo rohita (Hussain et al 2018), have been widely used to investigate DNA damage caused by heavy metals.…”

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confidence: 99%

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Genotoxic Effects of Environmental Pollutant Heavy Metals on Alburnus chalcoides (Pisces: Cyprinidae) Inhabiting Lower Melet River (Ordu, Turkey)

Kontaş

Bostancı

2020

Bull Environ Contam Toxicol

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The lower Melet River is a drinking water source that is surrounded by hazelnut grove, agricultural lands, resulting in the accumulation of genotoxic agents such as mining activities, various domestic and agricultural wastes. Therefore, it receives many domestic and agricultural wastes that contain the genotoxic agent. This study was aimed to assess the heavy metal concentrations in water, sediment, and bioaccumulation in the tissues of Alburnus chalcoides. Comet assay and micronucleus test were used to evaluate the genotoxic effects on the blood cells of A. chalcoides. The concentrations of heavy metals and metalloid in the water, in the sediments and in the muscle of fish were in the

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Genotoxic Effects of Environmental Pollutant Heavy Metals on Alburnus chalcoides (Pisces: Cyprinidae) Inhabiting Lower Melet River (Ordu, Turkey)

Kontaş

Bostancı

2020

Bull Environ Contam Toxicol

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“…Besides zebrafish as the fish model that lives in a freshwater environment, several other predominantly freshwater as well as brackish water species are used for the assessment of different contaminants using the comet assay; they include brown trout (Salmo trutta fario) [85], marble trout (Salmo marmoratus) [124], trout (Salmo cenerinus) [124], rainbow trout (Oncorhynchus mykiss) [125,126], common carp (Cyprinus carpio) [76, 82,127], major carp (Catla catla) [128], mrigal carp (Cirrhinus mrigala) [128], gibel carp (Carassius auratus gibelio) [129], common barbell (Barbus barbus) [130], common bleak (Alburnus alburnus) [131,132], freshwater bream (Abramis brama) [133], silver bream (Abramis bjoerkna) [134], white-eye bream (Abramis sapa) [134], rohu (Labeo rohita) [135][136][137][138][139], orangefin labeo (Labeo calbasu) [86], brown bullhead (Ameiurus nebulosus) [76,140], chub (Leuciscus cephalus) [141][142][143], European chub (Squalius cephalus) [87,[144][145][146], pale chub (Zacco platypus) [147], climbing perch (Anabas testudineus) [148], small-scaled pacu (Piaractus mesopotamicus) [149,150], eastern mosquitofish (Gambusia holbrooki) [77], Nile tilapia (Oreochromis niloticus) [151][152][153]…”

Section: Accepted Manuscriptmentioning

confidence: 99%

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Gajski

Žegura

Ladeira

et al. 2019

Mutation Research/Reviews in Mutation Research

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The comet assay has become one of the methods of choice for the evaluation and measurement of DNA damage. It is sensitive, quick to perform and relatively affordable for the evaluation of DNA damage and repair at the level of individual cells. The comet assay can be applied to virtually any cell type derived from different organs and tissues. Even though the comet assay is predominantly used on human cells, the application of the assay for the evaluation of DNA damage in yeast, plant and animal cells is also quite high, especially in terms of biomonitoring. The present extensive overview on the usage of the comet assay in animal models will cover both terrestrial and water environments. The first part of the review was focused on studies describing the comet assay applied in invertebrates. The second part of the review, (Part 2) will discuss the application of the comet assay in vertebrates covering cyclostomata, fishes, amphibians, reptiles, birds and mammals, in addition to chordates that are regarded as a transitional form towards vertebrates. Besides numerous vertebrate species, the assay is also performed on a range of cells, which includes blood, liver, kidney, brain, gill, bone marrow and sperm cells. These cells are readily used for the evaluation of a wide spectrum of genotoxic agents both in vitro and in vivo. Moreover, the use of vertebrate models and their role in environmental biomonitoring will also be discussed as well as the comparison of the use of the comet assay in vertebrate and human models in line with ethical principles. Although the comet assay in vertebrates is most commonly used in laboratory animals such as mice, rats and lately zebrafish, this paper will only briefly review its use regarding laboratory animal models and rather give special emphasis to the increasing usage of the assay in domestic and wildlife animals as well as in various ecotoxicological studies.

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“…The cell lysate was quantified using ICP‐OES. ICP‐OES is a highly sophisticated analytical technique to estimate trace and heavy metals in diverse environmental matrices . This study involved the evaluation of lead using Thermo Fisher Scientific iCAP 7400 ICP‐OES equipped with an autosampler.…”

Section: Methodsmentioning

confidence: 99%

Removal of Pb (II), As (III), and Cr (VI) by nitrogen‐starved Papiliotrema laurentii strain RY1

et al. 2019

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Heavy metals such as lead, chromium, and metalloid like arsenic dominate the pinnacle in posing a threat to life. Being environment‐friendly, elucidating the mechanism by which microorganisms detoxify such elements has always been an active field of research hitherto. In the present study, we have investigated the capability of nitrogen‐deprived Papiliotrema laurentii strain RY1 toward enhanced tolerance and neutralizing toxic elements. There were biosorption and bioprecipitation of lead and chromium at the cell surfaces. Bioprecipitation mechanisms included the formation of lead phosphates and pyromorphites from lead, grimaldite from chromium. Transcripts such as metallothionein, aquaporins, and arsenical pump‐driving ATPase have been surmised to be involved in the detoxification of elements. Furthermore, activation of antioxidant defense mechanisms for the cells for each of the elements should contribute towards yeast's propagation. The efficiency of removal of elements for live cells and immobilized cells were high for lead and chromium. To the best of our knowledge, this is the first report of such high tolerance of lead, arsenic, and chromium for any yeast. The yeast showed such varied response under dual stress due to nitrogen starvation and in the presence of respective elements. The yeast possesses promising potentials in nitrogen deprived and enriched environments to aid in bioremediation sectors.

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Assessment of status of three water bodies in Serbia based on tissue metal and metalloid concentration (ICP-OES) and genotoxicity (comet assay)

Cited by 32 publications

References 31 publications

Genotoxic Effects of Environmental Pollutant Heavy Metals on Alburnus chalcoides (Pisces: Cyprinidae) Inhabiting Lower Melet River (Ordu, Turkey)

Genotoxic Effects of Environmental Pollutant Heavy Metals on Alburnus chalcoides (Pisces: Cyprinidae) Inhabiting Lower Melet River (Ordu, Turkey)

The comet assay in animal models: From bugs to whales – (Part 2 Vertebrates)

Removal of Pb (II), As (III), and Cr (VI) by nitrogen‐starved Papiliotrema laurentii strain RY1

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