Assessment of the Accuprobe Listeria monocytogenes culture identification reagent kit for rapid colony confirmation and its application in various enrichment broths

Ninet, Béatrice Alice Bescher; Bannerman, Elisabeth; Billé, Jacques

doi:10.1128/aem.58.12.4055-4059.1992

Cited by 28 publications

(9 citation statements)

References 13 publications

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“…2) guarantees a strong, clear signal for detection of low numbers of injured L. monocytogenes cells. Combining the PSU broth system with modern molecular methods (38,39,50) should result in a hybrid system that is simple, inexpensive, and highly specific for the detection of heat-injured L. monocytogenes in foods containing background microflora. The strategy outlined in this paper might also be applied to the detection of numerous other facultatively anaerobic, food-borne pathogens, including Salmonella spp., Escherichia coli, Staphylococcus aureus, Shigella spp., Yersinia spp., Vibrio spp., etc.…”

Section: Discussionmentioning

confidence: 99%

A novel strictly anaerobic recovery and enrichment system incorporating lithium for detection of heat-injured Listeria monocytogenes in pasteurized milk containing background microflora

Mendonça

Knabel

1994

Appl Environ Microbiol

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Heat-injured cells of Listeria monocytogenes were recovered from heated raw milk containing noninjured Enterococcusfaecium by combining a simple method for obtaining strict anaerobiosis with a novel enrichment broth, Penn State University broth (PSU broth). Strictly anaerobic conditions were rapidly achieved by adding 0.5 g of filter-sterilized cysteine per liter to PSU broth and then purging the preparation with N2 gas. Little resuscitation or growth occurred in strictly anaerobic PSU broth without lithium chloride because of overgrowth by E. faecium. The growth ofE.faecium decreased dramatically with increasing LiCl concentration; LiCl concentrations of 8 and 10 g/liter were completely bacteriostatic. The mechanism of inhibition by LiCl appeared to involve competition with the divalent cations Ca2+ and Mg2+. Heat-injured L. monocytogenes consistently recovered and grew rapidly in strictly anaerobic PSU broth containing 4, 6, or 7 g of LiCl per liter. The use of strictly anaerobic PSU broth containing 7 g of LiCl per liter permitted detection of severely heat-injured L. monocytogenes in one simple recovery-enrichment step by eliminating oxygen toxicity and inhibiting the growth of background microflora, without preventing the resuscitation and subsequent growth of heat-injured L. monocytogenes. L. monocytogenes heated in raw milk at 62.8°C for 10, 15, and 20 min could be consistently recovered from strictly anaerobic PSU broth enrichment cultures at 30°C after 48, 96, and 144 h, respectively, and hence, use of PSU broth may result in better recovery of both injured and noninjured cells from foods than currently used U.S. Department of Agriculture and Food and Drug Administration preenrichment procedures.

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Section: Discussionmentioning

confidence: 99%

A novel strictly anaerobic recovery and enrichment system incorporating lithium for detection of heat-injured Listeria monocytogenes in pasteurized milk containing background microflora

Mendonça

Knabel

1994

Appl Environ Microbiol

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“…AccuProbe, phenotypic tests and growth range. The AccuProbe (Ninet et al, 1992;Okwumabua et al, 1992) test was performed according to the manufacturer's instructions (Gen-Probe). Liquid broth, semi-solid media and other plating media were incubated aerobically at 1, 4, 25, 30, 37, 43 or 45 uC.…”

Section: Methodsmentioning

confidence: 99%

Listeria marthii sp. nov., isolated from the natural environment, Finger Lakes National Forest

Graves

Helsel

Steigerwalt

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

199

129

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Four isolates (FSL S4-120 T , FSL S4-696, FSL S4-710, and FSL S4-965) of Gram-positive, motile, facultatively anaerobic, non-spore-forming bacilli that were phenotypically similar to species of the genus Listeria were isolated from soil, standing water and flowing water samples obtained from the natural environment in the Finger Lakes National Forest, New York, USA. The four isolates were closely related to one another and were determined to be the same species by whole genome DNA-DNA hybridization studies (.82 % relatedness at 55 6C and .76 % relatedness at 70 6C with 0.0-0.5 % divergence). 16S rRNA gene sequence analysis confirmed their close phylogenetic relatedness to Listeria monocytogenes and Listeria innocua and more distant relatedness to Listeria welshimeri, L. seeligeri, L. ivanovii and L. grayi. Phylogenetic analysis of partial sequences for sigB, gap, and prs showed that these isolates form a wellsupported sistergroup to L. monocytogenes. The four isolates were sufficiently different from L. monocytogenes and L. innocua by DNA-DNA hybridization to warrant their designation as a new species of the genus Listeria. The four isolates yielded positive reactions in the AccuProbe test that is purported to be specific for L. monocytogenes, did not ferment L-rhamnose, were nonhaemolytic on blood agar media, and did not contain a homologue of the L. monocytogenes virulence gene island. On the basis of their phenotypic characteristics and their genotypic distinctiveness from L. monocytogenes and L. innocua, the four isolates should be classified as a new species within the genus Listeria, for which the name Listeria marthii sp. nov. is proposed. The type strain of L. marthii is FSL S4-120 T (5ATCC BAA-1595 T 5BEIR NR 9579 T 5CCUG 56148 T ). L. marthii has not been associated with human or animal disease at this time.

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“…Cross-reactivity with competitors (Enterococcus spp., Staphylococcus spp. and bther listeria) was not observed with the Accuprobe (Bobbit and Betts 1992;Ninet et al 1992;Herman and de Ridder 1993) nor with the Gene-Trak DNA (Anon. 1995).…”

Section: Discussionmentioning

confidence: 99%

“…In further identification, traditional biochemical tests are used, including nitrate reduction and fermentation ofa range ofsugars (Lovett 1988). Newer methods for the confirmation of suspected colonies are based on latex tests and DNA probes (Ninet et al 1992). For identification on species level, systems based on biochemical tests are available .…”

Section: Introductionmentioning

confidence: 99%

Confirmation and identification of Listeria spp.

Beumer

Giffel

Kok

et al. 1996

Lett Appl Microbiol

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All confirmation and identification methods used in this study can be used for the screening of suspected colonies on isolation media for Listeria spp. In traditional enrichment procedures the Microscreen Listeria latex test gives fast results. The DNA probes (Accuprobe and Gene-Trak) are very specific in detecting Listeria monocytogenes. For identification of Listeria spp. both tests (API and Micro-ID) performed equally well. Preference may be given to the API test, since differentiation of L. monocytogenes from L. innocua is based on the absence of arylamidase, through which tests for haemolytic activity and/or CAMP reactions can be omitted. However, the use of Enhanced Haemolysis Agar as isolation medium makes further testing essentially superfluous, since L. monocytogenes strains can be differentiated from L. innocua.

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Assessment of the Accuprobe Listeria monocytogenes culture identification reagent kit for rapid colony confirmation and its application in various enrichment broths

Cited by 28 publications

References 13 publications

A novel strictly anaerobic recovery and enrichment system incorporating lithium for detection of heat-injured Listeria monocytogenes in pasteurized milk containing background microflora

A novel strictly anaerobic recovery and enrichment system incorporating lithium for detection of heat-injured Listeria monocytogenes in pasteurized milk containing background microflora

Listeria marthii sp. nov., isolated from the natural environment, Finger Lakes National Forest

Confirmation and identification of Listeria spp.

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