Assessment of the antioxidant, antimicrobial and antibiofilm activities of essential oils for potential application of active chitosan films in food preservation

Mouhoub, Anouar; Guendouz, Amine; Belkamel, Abdeljalil; Talibi, Zainab El Alaoui; Koraichi, Saâd Ibnsouda; Modafar, Cherkaoui El; Delattre, Cédric

doi:10.1007/s11274-022-03363-9

Cited by 17 publications

(6 citation statements)

References 91 publications

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“…In particular, the indirect activity of citronellal brings more possibilities to its application. It could also be combined with related technologies to improve the biological activity of plant extracts [53][54][55] to improve its stability and achieve a slow release of the drug. Although this study reveals the antifungal mechanism of citronellal against M. oryzae and preliminarily elucidated its control effect on rice blast, its molecular regulation mechanism and field application technology still require further study.…”

Section: Discussionmentioning

confidence: 99%

Natural Product Citronellal can Significantly Disturb Chitin Synthesis and Cell Wall Integrity in Magnaporthe oryzae

Zhou

et al. 2022

JoF

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Background: Natural products are often favored in the study of crop pests and diseases. Previous studies have shown that citronellal has a strong inhibition effect on Magnaporthe oryzae. The objective of this study was to clarify its mechanism of action against M. oryzae. Results: Firstly, the biological activity of citronellal against M. oryzae was determined by direct and indirect methods, and the results show that citronellal had a strong inhibition effect on M. oryzae with EC50 values of 134.00 mg/L and 70.48 μL/L air, respectively. Additionally, a preliminary study on its mechanism of action was studied. After citronellal treatment, electron microscopy revealed that the mycelium became thin and broken; scanning electron microscopy revealed that the mycelium was wrinkled and distorted; and transmission electron microscopy revealed that the mycelium cell wall was invaginated, the mass wall of mycelium was separated, and the organelles were blurred. The mycelium was further stained with CFW, and the nodes were blurred, while the mycelium was almost non-fluorescent after PI staining, and there was no significant difference in the relative conductivity of mycelium. In addition, chitinase was significantly enhanced, and the expression of chitin synthesis-related genes was 17.47-fold upregulated. Finally, we found that the efficacy of citronellal against the rice blast was as high as 82.14% according to indoor efficacy tests. Conclusion: These results indicate that citronellal can affect the synthesis of chitin in M. oryzae and damage its cell wall, thereby inhibiting the growth of mycelium and effectively protecting rice from rice blasts.

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Section: Discussionmentioning

confidence: 99%

Natural Product Citronellal can Significantly Disturb Chitin Synthesis and Cell Wall Integrity in Magnaporthe oryzae

Zhou

et al. 2022

JoF

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“…Thus, thymol could be a potent antibiofilm compound as other studies that have used thymol alone ( 68 ) or in synergistic combination with other antimicrobial agents ( 69 ) have reported a similar effect on biofilm formation. In addition to E. coli, other studies have observed antibiofilm activity of EOs against S. aureus ( 70 ), oral cariogenic bacteria ( 71 ), and foodborne pathogens ( 72 , 73 ) in vitro , showing the potential EOs have against persistent pathogenic biofilm formers in diverse environments. E. coli UMN026 was selected for this biofilm assay because bacterial BRD-associated pathogens do not normally produce biofilms; however, the NS microbiome contains biofilm-forming species, and these bacteria can influence resistance or susceptibility to the development of clinical BRD.…”

Section: Discussionmentioning

confidence: 99%

Screening and selection of essential oils for an intranasal spray against bovine respiratory pathogens based on antimicrobial, antiviral, immunomodulatory, and antibiofilm activities

Amat,

Magossi,

Rakibuzzaman

et al. 2024

Front. Vet. Sci.

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IntroductionThe rise in antibiotic resistant pathogens associated with bovine respiratory disease (BRD) poses a serious challenge, particularly to the beef feedlot industry, as they currently depend on antibiotics to prevent BRD to mitigate the financial burden (approx. $1 billion annual loss) inflicted by BRD-associated high mortality and morbidity in feedlot cattle. Thus, there is an impetus need for the development of antimicrobial alternative strategies against BRD. This study aimed to screen and select candidate essential oils (EOs) for the development of an intranasal EO spray that can inhibit BRD pathogens and promote microbiota-mediated respiratory health.MethodsThe effects of selected EOs (ajowan, cinnamon leaf, citronella, grapefruit, fennel, and thyme) on a bovine nasopharyngeal microbiota culture were evaluated using 16S rRNA gene sequencing. The microbiota culture was enriched by incubating nasopharyngeal swabs obtained from finishing beef heifers in brain heart infusion broth with and without EOs (0.025%, v/v). These EOs were then also evaluated for their immunomodulatory effects on bovine turbinate (BT) cells by analyzing the concentrations of 15 cytokines and chemokines in cell culture after 24 h incubation. The crystal violet assay was done to assess the antibiofilm activity of EOs against Escherichia coli UMN026 strain. Finally, 15 EOs were screened for their antiviral activity against the bovine viral diarrhea virus 1 (BVDV-1) using BT cells and a fluorescence-based method.ResultsAjowan, fennel, and thyme resulted in a moderate reduction of overall nasopharyngeal microbiota growth with significant alterations of both alpha and beta diversity, and the relative abundance of predominant bacterial families (e.g., increasing Enterobacteriaceae and decreasing Moraxellaceae) compared to the control (p < 0.05). Co-incubation of BT cells with selected EOs resulted in minimal alterations in cytokine and chemokine levels (p > 0.05). Ajowan, thyme, fennel, and cinnamon leaf exhibited antibiofilm activity at concentrations of 0.025 and 0.05%. Reduction of BVDV-1 replication in BT cells was observed with thyme (strong), and ajowan and citronella (moderate) at 0.0125% concentration.DiscussionAccordingly, ajowan, thyme, fennel, cinnamon leaf, and citronella EOs were selected for further development as an intranasal EO spray to prevent and control of BRD pathogens in feedlot cattle.

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“…Essential oils, volatile aromatic compounds extracted from various plant parts, have been used as complementary and alternative therapies in ancient civilizations, such as Egypt, China, and India, for at least 6,000 years. These oils possess numerous pharmacological properties, including anti-inflammatory, antinociceptive, antibacterial, antifungal, and antioxidant properties [22]. They have been widely used in inhalation aromatherapy, alleviating various mental symptoms, such as stress, anxiety, and depression [23].…”

Section: Discussionmentioning

confidence: 99%

Role of p-anisaldehyde in the Differentiation of C2C12 Myoblasts

KIM,

KONG,

CHO

et al. 2023

Korean J Clin Lab Sci

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In this study, we investigated whether p-anisaldehyde (PAA), the main component of essential oils derived from anise seeds, influences the differentiation of mouse C2C12 myoblasts. Cells were induced to differentiate over 5 days using a differentiation medium with or without PAA (50 or 200 mg/mL). Myotube length and diameter were measured, and the expressions of myogenic markers (myoblast determination protein 1, myogenin, myocyte enhancer factor 2, muscle creatine kinase, and myosin heavy chain) and atrophy-related genes (atrogin-1 and muscle ring finger-1 [MuRF-1]) were assessed by quantitative real-time polymerase chain reaction. Additionally, protein kinase B (Akt) phosphorylation was monitored by western blotting. PAA significantly induced the formation of smaller and thinner myotubes and reduced myogenic marker expression. Furthermore, PAA increased the expressions of atrogin-1 and MuRF-1 and simultaneously reduced Akt phosphorylation. Our findings indicate that PAA inhibits the myogenic differentiation of C2C12 cells by reducing the phosphorylation and activation of Akt.

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Assessment of the antioxidant, antimicrobial and antibiofilm activities of essential oils for potential application of active chitosan films in food preservation

Cited by 17 publications

References 91 publications

Natural Product Citronellal can Significantly Disturb Chitin Synthesis and Cell Wall Integrity in Magnaporthe oryzae

Natural Product Citronellal can Significantly Disturb Chitin Synthesis and Cell Wall Integrity in Magnaporthe oryzae

Screening and selection of essential oils for an intranasal spray against bovine respiratory pathogens based on antimicrobial, antiviral, immunomodulatory, and antibiofilm activities

Role of p-anisaldehyde in the Differentiation of C2C12 Myoblasts

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