Assessment of the benzethonium chloride method for routine determination of protein in cerebrospinal fluid and urine.

Flachaire, E; Damour, O; Bienvenu, Jean-François; Aouiti, T; Later, R

doi:10.1093/clinchem/29.2.343

Cited by 14 publications

(3 citation statements)

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“…Benzethonium chloride denatures proteins in urine, resulting in a fine suspension that can be qualified turbidimetrically. In contrast to the statements of Résimont et al [1], all protein fractions are precipitated [3]. Despite all efforts made in the past, proteinuria assays probably never will get standardized [4].…”

mentioning

confidence: 85%

About the estimation of albuminuria based on proteinuria results

Delanghe

Oyaert

Buyzere

et al. 2022

Clinical Chemistry and Laboratory Medicine (CCLM)

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mentioning

confidence: 85%

About the estimation of albuminuria based on proteinuria results

Delanghe

Oyaert

Buyzere

et al. 2022

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…The supernatant was stored in aliquots at À80°C until further use. Urine protein/creatinine ratios were determined by estimating the urine protein and creatinine levels by benzethonium chloride 21 and Jaffe's/ enzymatic methods, 22 respectively. Peripheral blood was collected in EDTA-treated vacutainers and centrifuged at 500 g for 10 min at 4°C.…”

Section: Sample Preparationmentioning

confidence: 99%

Extracellular mitochondrial components as new biomarkers for lupus nephritis

Gowda,

Rana,

Kumar

et al. 2024

Lupus

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Major reason for mortality among systemic lupus erythematosus patients is renal failure due to the deposition of immune complexes in the glomeruli. Being a chronic disease with multiple relapses and remissions across the lifespan, it’s important to know the degree of nephritis for diagnosis as well as the long-term clinical management of the patients. Currently, renal biopsy is being used as the gold standard to diagnose and define the stages of the disease. However, renal biopsy being invasive only provides a localized picture of nephritis, and has the risk of bleeding. Additionally, it is also cost-intensive. Hence, a reliable, non-invasive biomarker is required for lupus nephritis. This study has evaluated extracellular mitochondrial components, including cell-free mitochondria, and cell-free mitochondrial DNA as probable biomarkers of the degree of nephritis. Both showed a significant correlation with proteinuria and protein-creatinine ratio. Our study substantiates their usage as clinical biomarkers of nephritis upon their validation in a larger cohort of lupus nephritis patients and other forms of nephritis. Although the current data suggest using cell-free mitochondria as a biomarker of lupus nephritis is better than the cell-free mitochondrial DNA.

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“…Finally, protein concentration was determined by turbidimetric assay, based on the benzethonium chloride method using COBAS INTEGRA (Roche Diagnostics GmbH, Penberg, Germany). 21…”

Section: Patients and Samples Collection And Preparationmentioning

confidence: 99%

Proteomic analysis in clear cell renal cell carcinoma: identification of differentially expressed protein by 2-D DIGE

et al. 2012

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Renal cell carcinoma (RCC), the most common neoplasm affecting the adult kidney, is characterised by heterogeneity of histological subtypes, drug resistance, and absence of molecular markers. Two-dimensional difference gel electrophoresis (2-D DIGE) technology in combination with mass spectrometry (MS) was applied to detect differentially expressed proteins in 20 pairs of RCC tissues and matched adjacent normal kidney cortex (ANK), in order to search for RCC markers. After gel analysis by DeCyder 6.5 and EDA software, differentially expressed protein spots were excised from Deep Purple stained preparative 2DE gel. A total of 100 proteins were identified by MS out of 2500 spots, 23 and 77 of these were, respectively, over- and down-expressed in RCC. The Principal Component Analysis applied to gels and protein spots exactly separated the two sample classes in two groups: RCC and ANK. Moreover, some spots, including ANXA2, PPIA, FABP7 and LEG1, resulted highly differential. The DIGE data were also confirmed by immunoblotting analysis for these proteins. In conclusion, we suggest that applying 2-D DIGE to RCC may provide the basis for a better molecular characterization and for the discovery of candidate biomarkers.

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Assessment of the benzethonium chloride method for routine determination of protein in cerebrospinal fluid and urine.

Cited by 14 publications

References 0 publications

About the estimation of albuminuria based on proteinuria results

About the estimation of albuminuria based on proteinuria results

Extracellular mitochondrial components as new biomarkers for lupus nephritis

Proteomic analysis in clear cell renal cell carcinoma: identification of differentially expressed protein by 2-D DIGE

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