Assessment of the Biosafety of Microorganisms and their Joint Composition

Abstract

This publication presents the results of studying the biosafety of microorganisms used for the development of a biological product-a litter destructor, both individually and in a joint composition. In the experiment, the culture of Stylonychia (Stylonychia mytilus) and laboratory animals (rabbits, mice, and rats) were used. Based on a series of preclinical studies, it was found that the studied microorganisms individually do not indicate any symptoms of virulence, toxigenicity, toxicity and do not give an affirmative counteraction to the formation of hemolysin. The results of the express method showed that the aqueous and acetone extracts obtained from the joint microbial composition did not have a detrimental effect on the culture of protozoa, the viability in all groups was 100.0%. The analysis of the dermonecrotic effect of the studied microbial preparation demonstrated that the studied extracts did not cause hemorrhage, hyperemia, peeling, edema, and other pathology indicating an inflammatory process on the skin of laboratory rabbits. Conducting an acute experiment confirmed the safety of the studied microbial consortium, since during the research the laboratory mice remained active, mobile, no signs of poisoning or other pathology were found, and the conducted pathoanatomical autopsy did not reveal lesions of the parenchymal organs of the laboratory test objects. In general, the results of the studies indicated that the investigated cultures of microorganisms and their joint composition in the composition of the biological product-destructor do not show a toxic impact on the body of lab test objects, which confirms their harmlessness.

Animal Experiments and Laboratory Safety within the Scope of Occupational Health and Biosafety

“…In order to obtain reliable and sustainable results, genetically and microbiologically defined pedigrees are needed, as well as having a good laboratory animal laboratory. Moreover, infectious diseases in experimental animals must be kept under control and safe conditions must be provided for researchers and other employees (18)(19)(20)(21)(22). Biological risk assessment is a key factor in safe laboratory work.…”

“…The main risks that may arise when safe conditions are partially or completely eliminated are classified as follows (21,(23)(24)(25):  Direct skin, eye, or mucosal membrane exposure to an agent  Bites from a contaminated laboratory sharps instrument or infected animals and arthropod vectors  Ingestion of a liquid suspension of an infectious agent or exposure from contaminated hand-to-mouth  Inhalation of infectious aerosols The dangers arising from these phenomena, called risks, can be grouped under five main headings (21,26,27):…”