Assessment of the contribution of chemoreceptor‐based signalling to biofilm formation

Corral-Lugo, Andrés; Torre, J. de la; Matilla, Miguel A.; Fernández, Matilde; Morel, Bertrand; Espinosa‐Urgel, Manuel; Krell, Tino

doi:10.1111/1462-2920.13170

Cited by 74 publications

(63 citation statements)

References 94 publications

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“…Ligand binding to a protein causes typically Tm changes, which gives useful initial information as to the identification of potential ligands. This assay has been essential to gain initial insight into the specificity of several chemoreceptors (McKellar et al, 2015; Corral-Lugo et al, 2016; Fernandez et al, 2016). …”

Section: Resultsmentioning

confidence: 99%

“…Examples of the former group are receptors for different L -amino acids (Taguchi et al, 1997; Glekas et al, 2012; Oku et al, 2012; Brennan et al, 2013; Rico-Jimenez et al, 2013; Webb et al, 2016), cyclic organic acids (Luu et al, 2015), purines (Fernandez et al, 2016), polyamines (Corral-Lugo et al, 2016), aromatic hydrocarbons (Lacal et al, 2011b), C4–C6 organic acids (Lacal et al, 2010a; Parales et al, 2013) or C2- and C3-organic acids (Garcia et al, 2015). Chemoreceptors that appear to respond to a single compound include the citrate specific chemoreceptors Tcp of Salmonella typhimurium (Yamamoto and Imae, 1993) and McpQ of P. putida KT2440 (Martin-Mora et al, 2016), the malate specific receptor PA2652 in P. aeruginosa or the GABA specific McpG of P. putida KT2440 (Reyes-Darias et al, 2015a).…”

Section: Discussionmentioning

confidence: 99%

“…Examples are GABA, the only ligand of P. putida KT2440 McpG (Reyes-Darias et al, 2015a), and putrescine, the high-affinity ligand of McpU of the same species (Corral-Lugo et al, 2016). Both compounds serve as carbon and nitrogen sources and also exert functions as signaling molecules.…”

Section: Discussionmentioning

confidence: 99%

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Identification of a Chemoreceptor in Pseudomonas aeruginosa That Specifically Mediates Chemotaxis Toward α-Ketoglutarate

et al. 2016

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Pseudomonas aeruginosa is an ubiquitous pathogen able to infect humans, animals, and plants. Chemotaxis was found to be associated with the virulence of this and other pathogens. Although established as a model for chemotaxis research, the majority of the 26 P. aeruginosa chemoreceptors remain functionally un-annotated. We report here the identification of PA5072 (named McpK) as chemoreceptor for α-ketoglutarate (αKG). High-throughput thermal shift assays and isothermal titration calorimetry studies (ITC) of the recombinant McpK ligand binding domain (LBD) showed that it recognizes exclusively α-ketoglutarate. The ITC analysis indicated that the ligand bound with positive cooperativity (Kd1 = 301 μM, Kd2 = 81 μM). McpK is predicted to possess a helical bimodular (HBM) type of LBD and this and other studies suggest that this domain type may be associated with the recognition of organic acids. Analytical ultracentrifugation (AUC) studies revealed that McpK-LBD is present in monomer-dimer equilibrium. Alpha-KG binding stabilized the dimer and dimer self-dissociation constants of 55 μM and 5.9 μM were derived for ligand-free and αKG-bound forms of McpK-LBD, respectively. Ligand-induced LBD dimer stabilization has been observed for other HBM domain containing receptors and may correspond to a general mechanism of this protein family. Quantitative capillary chemotaxis assays demonstrated that P. aeruginosa showed chemotaxis to a broad range of αKG concentrations with maximal responses at 500 μM. Deletion of the mcpK gene reduced chemotaxis over the entire concentration range to close to background levels and wild type like chemotaxis was recovered following complementation. Real-time PCR studies indicated that the presence of αKG does not modulate mcpK expression. Since αKG is present in plant root exudates it was investigated whether the deletion of mcpK altered maize root colonization. However, no significant changes with respect to the wild type strain were observed. The existence of a chemoreceptor specific for αKG may be due to its central metabolic role as well as to its function as signaling molecule. This work expands the range of known chemoreceptor types and underlines the important physiological role of chemotaxis toward tricarboxylic acid cycle intermediates.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Identification of a Chemoreceptor in Pseudomonas aeruginosa That Specifically Mediates Chemotaxis Toward α-Ketoglutarate

et al. 2016

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“…Rhizobium leguminosarum , Pseudomonas fluorescens , Azotobacter chroococcum , Sinorhizobium meliloti , and many others are attracted by root exudates (5–9). For some of these, chemotaxis toward plant-secreted molecules is also required for establishing the initial host-microbe interaction (7, 10–15). Similarly, the causative agents of certain plant diseases, such as Ralstonia solanacearum and Dickeya dadantii 3937, necessitate directed motility mediated by chemotaxis for their plant virulence activity (16–18).…”

Section: Introductionmentioning

confidence: 99%

Bacillus subtilis Early Colonization of Arabidopsis thaliana Roots Involves Multiple Chemotaxis Receptors

Allard-Massicotte

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Lécuyer

et al. 2016

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Colonization of plant roots by Bacillus subtilis is mutually beneficial to plants and bacteria. Plants can secrete up to 30% of their fixed carbon via root exudates, thereby feeding the bacteria, and in return the associated B. subtilis bacteria provide the plant with many growth-promoting traits. Formation of a biofilm on the root by matrix-producing B. subtilis is a well-established requirement for long-term colonization. However, we observed that cells start forming a biofilm only several hours after motile cells first settle on the plant. We also found that intact chemotaxis machinery is required for early root colonization by B. subtilis and for plant protection. Arabidopsis thaliana root exudates attract B. subtilis in vitro, an activity mediated by the two characterized chemoreceptors, McpB and McpC, as well as by the orphan receptor TlpC. Nonetheless, bacteria lacking these chemoreceptors are still able to colonize the root, suggesting that other chemoreceptors might also play a role in this process. These observations suggest that A. thaliana actively recruits B. subtilis through root-secreted molecules, and our results stress the important roles of B. subtilis chemoreceptors for efficient colonization of plants in natural environments. These results demonstrate a remarkable strategy adapted by beneficial rhizobacteria to utilize carbon-rich root exudates, which may facilitate rhizobacterial colonization and a mutualistic association with the host.

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“…Furthermore, chemotaxis to GABA and polyamines was associated with root colonization in Pseudomonas putida KT2440 (36,37). Taken together, this knowledge forms the rational basis for attempts to enhance the colonization of plants by beneficial bacteria.…”

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confidence: 82%

Recent Advances and Future Prospects in Bacterial and Archaeal Locomotion and Signal Transduction

et al. 2017

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The structure and function of two-component and chemotactic signaling and different aspects related to the motility of bacteria and archaea are key research areas in modern microbiology. Escherichia coli is the traditional model organism used to study chemotaxis signaling and motility. However, the recent study of a wide range of bacteria and even some archaea with different lifestyles has provided new insight into the ecophysiology of chemotaxis, which is essential for the establishment of different pathogens or beneficial bacteria in a host. The expanded range of model organisms has also permitted the study of chemosensory pathways unrelated to chemotaxis, multiple chemotaxis pathways within an organism, and new types of chemoreceptors. This research has greatly benefitted from technical advances in the field of cryomicroscopy, which continues to reveal with increasing resolution the complexity and diversity of large protein complexes like the flagellar motor or chemoreceptor arrays. In addition, sensitive instruments now allow an increasing number of experiments to be conducted at the single-cell level, thereby revealing information that is beginning to bridge the gap between individual cells and population behavior. Evidence has also accumulated showing that bacteria have evolved different mechanisms for surface sensing, which appears to be mediated by flagella and possibly type IV pili, and that the downstream signaling involves chemosensory pathways and two-component-system-based processes. Herein, we summarize the recent advances and research tendencies in this field as presented at the latest Bacterial Locomotion and Signal Transduction (BLAST XIV) conference.KEYWORDS chemotaxis, flagella, flagellar motility, signal transduction, two-component regulatory systems T he capacity to sense and respond to changes in environmental cues is an essential feature of the prokaryotic lifestyle. As a consequence, bacteria and archaea have evolved an array of different molecular mechanisms that permit the detection of signals in order to generate appropriate cellular responses. These responses are mediated primarily by one-and two-component systems, as well as chemosensory signaling pathways (1-4). Whereas the former systems mediate changes primarily at the transcriptional level, chemosensory pathways form the basis for chemotaxis, the directed movement of prokaryotes in compound gradients. The study of signaling processes is not only of fundamental interest but may also contribute to the tackling of one of the central clinical problems, which is the increasing amount of antibiotic-resistant pathogens. There is now a significant amount of data indicating that interference with signal transduction systems, motility, and chemotaxis can be an alternative strategy to weaken or block pathogens (5, 6).In

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Assessment of the contribution of chemoreceptor‐based signalling to biofilm formation

Cited by 74 publications

References 94 publications

Identification of a Chemoreceptor in Pseudomonas aeruginosa That Specifically Mediates Chemotaxis Toward α-Ketoglutarate

Identification of a Chemoreceptor in Pseudomonas aeruginosa That Specifically Mediates Chemotaxis Toward α-Ketoglutarate

Bacillus subtilis Early Colonization of Arabidopsis thaliana Roots Involves Multiple Chemotaxis Receptors

Recent Advances and Future Prospects in Bacterial and Archaeal Locomotion and Signal Transduction

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