Assessment of the cytotoxic, genotoxic, and mutagenic potential of Acrocomia aculeata in rats

Traesel, Giseli Karenina; Castro, Luis Henrique Almeida; Silva, P.V.B.; Muzzi, Rozanna Marques; Kassuya, Cândida Aparecida Leite; Arena, Arielle Cristina; Oesterreich, Silvia Aparecida

doi:10.4238/2015.january.26.13

Cited by 18 publications

(11 citation statements)

References 31 publications

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“…Previous findings from our group indicated that OPAC did not exhibit cytotoxic, genotoxic or mutagenic effects in rats (Traesel et al, 2014), which corroborates with the results of the present study. These results demonstrate the absence of acute and subacute toxicity as a consequence of the oral treatment with A. aculeata oil, suggesting that their oral LD50 is higher than 2000 mg/kg.…”

Section: Resultssupporting

confidence: 96%

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Acute and subacute (28 days) oral toxicity assessment of the oil extracted from Acrocomia aculeata pulp in rats

Traesel

Souza

Barros

et al. 2014

Food and Chemical Toxicology

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Acrocomia aculeata, popularly known as “bocaiúva”, is a species used for nutritional purposes and for the treatment of various diseases, as it has, among other things, high levels of antioxidant compounds. This study aimed to assess the toxicological profile of A. aculeata, through acute and subacute toxicity tests. Male and female rats (Wistar) received by gavage 2000 mg/kg of oil extracted from the pulp of A. aculeata (OPAC) for the acute toxicity test and 125, 250, 500 or 1000 mg/kg of OPAC for subacute toxicity test. In the acute toxicity study no mortality or behavioral changes were observed in rats treated with 2000 mg/kg, indicating that the LD50 is higher than this dose. In the subacute toxicity test, the tested doses produced no significant changes in hematological, biochemical or histopathological parameters in the animals exposed. These results demonstrate the absence of acute and subacute toxicity after oral exposure to A. aculeata oil in rats. However, further studies in animals and in humans are needed in order to have sufficient safety evidence for its use in humans.

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Section: Resultssupporting

confidence: 96%

“…Our research group has determined the chemical composition of fatty acid of methyl monoesters of the oil obtained from the pulp of A. aculeata (OPAC) through gas chromatography (GC) using methods of mass spectrometry detector (MS) and flame ionization detector (FI) (Traesel et al, 2014). The results are expressed in Table 1.…”

Section: Resultsmentioning

confidence: 99%

Acute and subacute (28 days) oral toxicity assessment of the oil extracted from Acrocomia aculeata pulp in rats

Traesel

Souza

Barros

et al. 2014

Food and Chemical Toxicology

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“…Their results showed that the median lethal dose (LD 50 ) was over 2000 mg/kg and that doses of 150-1000 mg/kg did not alter hematologic, biochemical, or histologic parameters. The lack of toxicity was also reported by Traesel et al (2015). This study established the LD 50 with doses above 2000 mg/kg and found a lack of genotoxicity and mutagenicity for doses of 125-2000 mg/kg.…”

Section: Discussionmentioning

confidence: 69%

“…The list of tested plants includes Acrocomia aculeata, a plant native to South and Central America with demonstrated medicinal (Traesel et al, 2014(Traesel et al, , 2015 and nutraceutical (Lescano et al, 2015) properties.…”

Section: Introductionmentioning

confidence: 99%

Acrocomia aculeata prevents toxicogenetic damage caused by the antitumor agent cyclophosphamide

Magosso¹,

Carvalho

Shneider³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Acrocomia aculeata is a plant rich in antioxidant compounds. Studies suggest that this plant has anti-inflammatory, antidiabetic, and diuretic potential. We assessed the antigenotoxic, antimutagenic, immunomodulation, and apoptotic potentials of A. aculeata alone and in combination with an antitumor agent, cyclophosphamide. Swiss male mice (N = 140) were used. The animals were divided into 14 experimental groups as follows: a negative group, a positive group (100 mg/kg cyclophosphamide), groups that only received the oil extracted from the almond (AO) and from the pulp (PO) of A. aculeata at doses of 3, 15, and 30 mg/kg, and the associated treatment groups (oils combined with cyclophosphamide) involving pretreatment, simultaneous, and post-treatment protocols. Data suggest that both oils were chemopreventive at all doses, based on the tested protocols. The highest damage reduction percentages, observed for AO and PO were 88.19 and 90.03%, respectively, for the comet assay and 69.73 and 70.93%, respectively, for the micronucleus assay. Both AO and PO demonstrated immunomodulatory activity. The oils reduced the capacity of cyclophosphamide to trigger apoptosis in the liver, spleen, and kidney cells. These results suggest that A. aculeate AO and PO can be classified as a functional food and also enrich other functional foods and nutraceuticals with chemopreventive features. However, they are not appropriate sources for chemotherapeutic adjuvants, in particular for those used in combination with cyclophosphamide.

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“…Besides, no oxidative stress‐mediated cytotoxic or genotoxic effects were observed in NIH3T3 fibroblasts after rapeseed oil (63% oleic acid content) exposure (Lewinska et al ). And treatment of Wistar rats with oil extracted from the pulp of Acrocomia aculeata (71% oleic acid content) did not produce cytotoxic, genotoxic, or mutagenic effects (evaluated by comet assay and MN test) (Traesel et al ). Complementarily, oleic acid has been reported to be an effective antimutagenic agent, preventing the mutagenicity of benzo(a)pyrene present in air‐borne particulates (Iwado et al ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of cytotoxicity and genotoxicity induced by oleic acid‐coated iron oxide nanoparticles in human astrocytes

Fernández-Bertólez

Costa

Brandão

et al. 2019

Environ and Mol Mutagen

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Iron oxide nanoparticles (ION) are gaining importance as diagnostic and therapeutic tool of central nervous system diseases. Although oleic acid‐coated ION (O‐ION) have been described as stable and biocompatible, their potential neurotoxicity was scarcely evaluated in human nervous cells so far. The primary aim of this work was to assess the molecular and cellular effects of O‐ION on human astrocytes (A172 cells) under different experimental conditions. An extensive set of cyto‐ and genotoxicity tests was carried out, including lactate dehydrogenase release assay, cell cycle alterations, and cell death production, as well as comet assay, γH2AX assay, and micronucleus (MN) test, considering also iron ion release capacity and alterations in DNA repair ability. Results showed a moderate cytotoxicity related to cell cycle arrest and cell death promotion, regardless of serum presence. O‐ION induced genotoxic effects, namely primary DNA damage, as detected by the comet assay and H2AX phosphorylation, but A172 cells were able to repair this particular damage because no chromosome alterations were found (confirmed by MN test results). Accordingly, no effects on the DNA repair ability were observed. The presence of serum proteins did not influence O‐ION toxicity. Iron ions released from the O‐ION surface seemed not to be responsible for the cytotoxic and genotoxic effects observed. Environ. Mol. Mutagen. 2019. © 2019 Wiley Periodicals, Inc.

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Assessment of the cytotoxic, genotoxic, and mutagenic potential of Acrocomia aculeata in rats

Cited by 18 publications

References 31 publications

Acute and subacute (28 days) oral toxicity assessment of the oil extracted from Acrocomia aculeata pulp in rats

Acute and subacute (28 days) oral toxicity assessment of the oil extracted from Acrocomia aculeata pulp in rats

Acrocomia aculeata prevents toxicogenetic damage caused by the antitumor agent cyclophosphamide

Evaluation of cytotoxicity and genotoxicity induced by oleic acid‐coated iron oxide nanoparticles in human astrocytes

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