Assessment of the EDTA seroagglutination test for the diagnosis of bovine brucellosis

Garin, B; Trap, D; Gaumont, R.

doi:10.1136/vr.117.17.444

Cited by 15 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Firstly, the binding of nonspecific agglutinins to brucella cells has been suggested as contributing to positive responses in the SAT. This problem could be overcome by the addition of EDTA to the agglutination suspension (13,21,26). Secondly, agglutinating antibodies could be elicited in animals infected with other pathogens which present antigenic cross-reactivity with bru- cella, such as Yersinia enterocolitica O:9 and Escherichia coli O:157 (8,20,23).…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of microagglutination test and serum agglutination test as supplementary diagnostic methods for brucellosis

Baum¹,

Zamir²,

Bergman-Rios³

et al. 1995

J Clin Microbiol

View full text Add to dashboard Cite

The diagnosis of brucellosis in cattle and small ruminants requires the use of more than one serological test. The complement fixation test (CFT), the rose bengal test (RBT), and the serum agglutination test (SAT) are among the most useful tests for routine diagnosis. The microagglutination test (MAT) was developed as a simpler and more efficient test than the SAT. The relative efficacy of this test compared with that of the SAT was evaluated by using brucella-free sheep and goats prior to and after vaccination treatment. The specificities of the MAT and the SAT were 100%. Of the ewes and goats with a vaccination history, one ewe, expectedly a negative responder, had reactions in the MAT, the complement fixation test, and the rose bengal test but not in the SAT, suggesting a lower sensitivity of the SAT in this case. The calculated sensitivities of the MAT and the SAT were 93.9%. The agreement between MAT and SAT results for nonresponders was examined by using sera from unvaccinated lambs and kids (95.2% agreement), unvaccinated ewes and goats (84.4%), and ewes and goats with a vaccination history (43.9%). For the latter group higher levels of agglutination units were observed by the MAT than by the SAT in 51.5% of the samples. In testing sera from positive reactors after vaccination neither method was superior (MAT values were greater than SAT values for 23.5% of the samples, and MAT values were less than SAT values for 21.9% of the samples). Comparison of the methods on the individual sample level revealed a significant correlation between the MAT and the SAT (r ‫؍‬ 0.96 ؎ 0.005; P < 0.001). Since the MAT is simpler to perform than the SAT and can potentially be automated, the inclusion of the MAT as a supplementary test in brucellosis control programs is recommended.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of microagglutination test and serum agglutination test as supplementary diagnostic methods for brucellosis

Baum¹,

Zamir²,

Bergman-Rios³

et al. 1995

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…MAT was performed with EDTA as described by Garin et al (1985). The antigen used was B. abortus biotype 1 (Weybridge 99) (Synbiotics Europe, France).…”

Section: Micro Agglutination Test (Mat)mentioning

confidence: 99%

Risk Factors Associated With Prevalence of Brucellosis in Black Bengal Goats in Bangladesh

Islam

Samad

Rahman

2012

Bangl. J. Vet. Med.

View full text Add to dashboard Cite

A total of 242 milk and 208 blood samples of goat were collected from three organized goat farms and surrounding rural areas of Bangladesh Agricultural University to determine the prevalence and associated risk factors of brucellosis in Black Bengal goats during the period from December 2008 to September 2009. Milk samples were screened by Milk Ring Test (MRT) and serum samples by Rose Bengal test (RBT) and Micro Agglutination Test (MAT) for detection of brucella specific antibody in milk and blood respectively. The overall prevalence was recorded as 13.64% in milk by MRT; 3.85% and 3.37% in serum by RBT and MAT respectively. About 21.21(7/33) % and18.18 (6/33) % of MRT positive goat showed positive reactions in RBT and MAT respectively. Does aged up to 4 years had lower prevalence (3.70%) of brucellosis than those aged over 4 years (12.50%). About 2.1 (odds ratio, OR = 2.1; 95% CI: 1.21-4.53) and 47.1(OR = 47.1; 95% CI: 5.3-416.6) folds increased odds of seropositivity of brucellosis were observed in aborted and placental retention cases respectively. Significantly (p<0.05) higher prevalence of brucellosis was recorded at late lactation stage (17.94%) than those were in mid (16%) and early lactation stage (11.76%). A significantly higher odds of seropositivity of brucellosis was observed in does (OR = 23; 95% CI: 3.08-173.62). About 7 folds (OR = 6.8; 95% CI: 1.13-5.32) increased odds of seropositivity was observed in pregnant does.

show abstract

“…Because of the lack of specificity of the SAT, an adaptation of the test which includes the addition of ethylene diaminotetraacetic acid disodium salt has proven to significantly increase test specificity [61][62][63]. The mechanism by which EDTA reduces non specificity is not understood, however, it appears to eliminate attachment of immunoglobulins to the Brucella cell wall via the Fc piece.…”

Section: Use Of Edtamentioning

confidence: 99%

Diagnosis of Brucellosis

Poester¹,

Nielsen²,

Samartino³

et al. 2010

TOVSJ

111

View full text Add to dashboard Cite

Brucellosis is an important zoonosis and a significant cause of reproductive losses in animals. Abortion, placentitis, epididymitis, and orchitis are the most common clinical manifestations in animals. In humans, brucellosis is a debilitating and chronic disease, which may affect a variety of organs. Clinical diagnosis of brucellosis is not easily achieved. Laboratory testing is therefore very important for a correct identification of the disease in humans and for the detection and confirmation in animals. Definitive diagnosis is normally done by isolation and identification of the causative agent. While definitive, isolation is time-consuming, must be performed by highly skilled personnel, and it is hazardous. For these reasons, serological tests are normally preferred. Brucellosis serology have advanced considerably in the last decades with very sensitive and specific new tests available. Modern genetic characterization of Brucellae using molecular DNA technology have been developed. Several PCR-based assays have been proposed, from the rapid recognition of genus to differential identification of species and strains. This review describes bacteriological, serological, and molecular methods used for the diagnosis of human and animal brucellosis.

show abstract

Assessment of the EDTA seroagglutination test for the diagnosis of bovine brucellosis

Cited by 15 publications

References 0 publications

Comparative evaluation of microagglutination test and serum agglutination test as supplementary diagnostic methods for brucellosis

Comparative evaluation of microagglutination test and serum agglutination test as supplementary diagnostic methods for brucellosis

Risk Factors Associated With Prevalence of Brucellosis in Black Bengal Goats in Bangladesh

Diagnosis of Brucellosis

Contact Info

Product

Resources

About