Assessment of the Effect of Metabolic Syndrome on the Autophagy Marker LC3 in Psoriasis Vulgaris Patients: A Cross-Sectional Study

Abstract

Purpose Psoriasis vulgaris, one of the most prevalent chronic inflammatory skin diseases, is associated with metabolic syndrome (MetS). Autophagy, an intracellular degradation system is essential for cell survival and differentiation, and its dysfunction may contribute to metabolic diseases. A cross-sectional study was conducted on 38 psoriasis vulgaris patients and 16 healthy control subjects to 1) Assess immunohistochemical (IHC) expression of microtubule-associated protein light chain 3 (LC3); 2) Evaluate the relationship between Psoriasis Area Severity Index (PASI) score, and LC3 expression. Patients and Methods PASI score was evaluated for all psoriasis patients. Lipid profile, blood sugar, and CRP were done for all patients and controls. A punch biopsy was taken from lesional and perilesional skin of psoriasis patients and normal skin of the controls. Tissue sections were prepared. IHC LC3 staining was done and evaluated. Results LC3 was nearly absent, in the epidermis of the lesional skin of psoriasis while it was strong among control (p=0.001). LC3 expression in the lesional skin of psoriasis vulgaris was lower than its expression in perilesional (p=0.001). However, LC3 expression was not significantly changed with PASI or the presence/absence of MetS. Conclusion A potential link between psoriasis vulgaris and autophagy as assessed by LC3 could be present. LC3 was down-regulated in psoriasis lesions than in normal skin. However, its expression did not change with PASI or MetS. An autophagy enhancer might be used as a possible therapeutic target in psoriasis vulgaris patients.

Association between the severity of hard-to-treat psoriasis and the prevalence of metabolic syndrome: A hospital-based cross-sectional study in Jakarta, Indonesia

“…Overall proportions of hypertriglyceridemia and low HDL level were found higher compared to other studies [ 29 – 31 ]. Current studies identified HLA genes were associated with psoriasis and positively correlated with the risk of dyslipidemia.…”

Insights into Autophagic Machinery and Lysosomal Function in Cells Involved in the Psoriatic Immune-Mediated Inflammatory Cascade

“…Overexpression of p62 does not necessarily imply autophagy induction alone; it could also indicate autophagy inhibition, which needs to be verified by concurrent upregulation of LC3-II, another autophagy marker (Lee et al 2011;Li et al 2016). Indeed, a potential link between Ps and autophagy marker LC3 with lower skin expression in patients than in control was reported (Nada et al 2020).…”

“…While these techniques are more commonly used for other purposes, ongoing research is exploring their potential in detecting cellular dysfunction. Similar to blood tests, urine analysis can reveal the presence of specific compounds that Akinduro et al 2016;Balato et al 2014;Bocheńska et al 2019;Buerger 2018;Dombrowski et al 2011;Douroudis et al 2012;Farag et al 2019;Huang et al 2015;Johansen et al 2007;Klapan et al 2021;Lee et al 2011;Li et al 2016;Mahil et al 2016;Mercurio et al 2021;Monteleon et al 2018;Nada et al 2020;Paushter et al 2018;Salazar et al 2020;Salskov-Iversen et al 2011;Sánchez-Martín et al 2019;Schönefuß et al 2010;Sun et al 2014;Wang et al 2019b;Xue et al 2022;Yin et al 2018;Yu et al 2007 Bronietzki et al 2015;Chung et al 2009;Delgoffe et al 2009Delgoffe et al , 2011Deretic 2021;Dowling et al 2018;Harris et al 2008;Hirai et al 2013;Hubbard et al 2010;Jia et al 2015;Kabat et al 2016;Kiyono et al 2009;Koga et al 2014;...…”

The Relationship Between Alzheimer’s Disease and Skin Diseases: A Review

“…Psoriasis is an immune-mediated inflammatory skin disease characterized by chronic plaques covered by silvery-white scales. 59 , 60 This disease affects 0.5–11.4% adults, causing huge negative effects on their quality of life. 61–63 Nowadays, psoriasis is considered a systematic condition associated with many comorbidities: cardiovascular comorbidities, other immune-mediated diseases, and mental disorders.…”