Assessment of the Effect of Chitosan Nanoparticles on the Ultra-Structure  of Dentinal Wall: A Comparative In vitroStudy

Soliman, A. Y.; Roshdy, Nehal Nabil; Lutfy, Reem Ahmed

doi:10.31080/asds.2020.04.0806

Cited by 5 publications

(5 citation statements)

References 21 publications

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“…It can be explained that the ERB sealer employed in this study (AH 26) consisted of formaldehyde, epoxy resin, and hexamethylenetetramine, besides being antibacterial, they are also toxic. 23,27 This cytotoxic effect in this study was only observed after 24 hours. This effect may decline if the longer observation is undertaken because the release of formaldehyde is reduced on the 7th day.…”

Section: Discussionmentioning

confidence: 50%

“…It might be due to the presence of antibacterial components, such as hexamethylenetetramine and formaldehyde in the ERB sealer used in this study (AH 26). 23 The more concentration addition of the chitosan to ERB sealer may not affect the antibacterial activity as shown in this study. 24 ADTwas selected to evaluate antibacterial properties in this study since this technique has been widely used to evaluate the antibacterial activity of sealers and offers many advantages, such as simplicity, low cost, the ability to test enormous numbers of microorganisms and antimicrobial agents, and the ease to interpret results provided.…”

Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

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Antibacterial and Cytotoxicity of Root Canal Sealer with the Addition of Chitosan Nanoparticle at Various Concentrations

et al. 2022

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Objectives The aim of this study was to evaluate the addition of chitosan nanoparticle with concentrations of 0, 10, 20, and 30% to the epoxy resin-based (ERB) sealer on its antibacterial and cytotoxicity effect. Methods and Materials This research was divided into two studies, the first study was the addition of chitosan with a concentration of 0% (as control), 10, 20, and 30% to an ERB sealer on its antibacterial effect, and the second study was on its cytotoxicity. An agar diffusion test was employed to determine the antibacterial effect on Enterococcus faecalis. An MTT (3-{4,5-dimethylthiazol-2-yl}-2,5-diphenyl tetrazolium bromide) assay was utilized to test the cytotoxicity by evaluating cell viability. Statistical Analysis One-way analysis of variance and Tukey's test (α = 0.05) were used to analyze data obtained from each evaluation with a significance level of 95%. Results The addition of chitosan nanoparticles at concentrations 10, 20, and 30% produced a greater inhibition zone of E. faecalis (p < 0.05), however, had less cytotoxicity compared with no addition of chitosan (0%) (p < 0.05). Conclusion The addition of chitosan nanoparticles at concentrations 10, 20, and 30% to the ERB sealer produced greater antibacterial and less cytotoxicity compared with no addition of chitosan (0%).

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Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

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Antibacterial and Cytotoxicity of Root Canal Sealer with the Addition of Chitosan Nanoparticle at Various Concentrations

et al. 2022

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“…The explanation of these results can be demonstrated by higher density of tubules within the coronal third compared to the middle as well as apical thirds [56] . Added to that, the impact of EDTA on the removal of smear layer declines while moving from coronal to apical [57] . This could be associated with increased number of opened dentinal tubules in the coronal third therefore more resin tags and higher AH plus bond [52,54] .…”

Section: Discussionmentioning

confidence: 99%

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2023

Int. J. Appl. Dent. Sci.

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The objective of this study was to determine and compare the Push-out bond strength of AH Plus sealer of root canal following prior application of calcium hydroxide (Ca[OH]2) paste and Nano-silver gel as intracanal medication in single-rooted human extracted maxillary incisors. Methodology: Using of ProTaper System up till master apical file # F5 have been carried out to prepare root canals of 30 human extracted maxillary incisors. According to the intracanal medication applied, division of teeth were carried out in random manner into three groups. Group I: Control group (No intracanal medication), Group II: Nano-silver gel and Group III: Calcium hydroxide Ca(OH)2. Three weeks later, the elimination process of the intracanal medications were achieved by using passive ultrasonic irrigation of 5 mL 2.5% NaClO, in addition to that, a 17% EDTA was used in the step of final flush for duration of 60 seconds succeeded by 5 mL of distilled water. Using the master cone #F5 and AH plus sealer, the single cone approach was used to fill the canals. After 1 week, the universal testing machine was implemented to test the push out-bond strength of the samples, which was performed for a 2-mm-thick of coronal, middle and apical thirds of each root. Results: A remarkable difference was evident between the control group and the comparison groups (p<0.001). Within the control group, the greatest value was evident (4.85±1.65), followed by calcium hydroxide (2.11±1.17), meanwhile the lowest value was discovered in nano-silver (1.41±1.08). Post hoc pairwise comparisons demonstrated a remarkably higher value in the control group than the rest of groups (p<0.001) with no statistically remarkable difference between calcium hydroxide and Nano-silver group. Conclusion:Prior calcium hydroxide and nano-silver placement seemed to decrease the push-out bond strength of epoxy resin-based Ah plus sealer.

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The Effect of Final Irrigation Protocols on the Apical Sealing Ability of Epoxy Resin-based and Bioceramic-based Root Canal Sealers

Medhat

Ghoneim

Roshdy

2022

Open Access Maced J Med Sci

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Aim: This study was designed to investigate the effect of different final irrigation protocols on the apical sealing ability of bioceramic and epoxy resin-based sealers. Materials and methods: Thirty human single-rooted mandibular premolars were instrumented using ProTaper Next rotary files. Teeth were randomly divided into three groups according to the final irrigation regimen; Group I: 5 ml 0.2% CNPs/3 min, Group II: 2.5 ml 0.2% CNPs/1.5 min followed by 2.5 ml 17% EDTA/1.5 min, and Group III: 5 ml 17% EDTA/3min. All groups were subdivided into two subgroups based on the obturation material; Subgroup A: gutta-percha/Sure-Seal Root BC Sealer and Subgroup B: gutta-percha/AH Plus. All canals were obturated using single cone obturation technique. The apical sealing ability was assessed using modified silver staining technique with ammoniacal silver nitrate tracer solution. Samples were sectioned longitudinally and examined using scanning electron microscope. Results: Sure-Seal Root BC sealer showed significantly lower nanoleakage compared to AH Plus (p < 0.001). No significant difference was recorded in the nanoleakage of Sure-Seal Root BC sealer among the three groups (p = 0.284), while AH Plus showed a significantly higher nanoleakage in the EDTA group (p = 0.002). The depth of silver nitrate penetration into the dentinal tubules was significantly higher in AH Plus subgroup with the three different irrigation protocols (p < 0.001). For both sealers, the highest penetration depth for silver nitrate tracer solution was recorded in the EDTA group (p < 0.001). Conclusions: The apical sealing ability of bioceramic sealers is better than that of epoxy resin-based sealers. The type of the final irrigating solution seems to affect the post-obturation seal of both AH Plus and Sure-Seal Root BC sealer. Keywords: Bioceramic, AH Plus, Chitosan nanoparticles, EDTA, Sealing ability.

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Assessment of the Effect of Chitosan Nanoparticles on the Ultra-Structure of Dentinal Wall: A Comparative In vitroStudy

Cited by 5 publications

References 21 publications

Antibacterial and Cytotoxicity of Root Canal Sealer with the Addition of Chitosan Nanoparticle at Various Concentrations

Antibacterial and Cytotoxicity of Root Canal Sealer with the Addition of Chitosan Nanoparticle at Various Concentrations

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The Effect of Final Irrigation Protocols on the Apical Sealing Ability of Epoxy Resin-based and Bioceramic-based Root Canal Sealers

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