A feeding trial was conducted to evaluate the effects of replacing soybean meal (SBM) with rubber seed meal (RSM) on digestive enzyme activity, nutrient digestibility and retention in juvenile tilapia (Oreochromis niloticus 9 Oreochromis aureus). Five isonitrogenous and isoenergetic diets were formulated with 0 (control), 100, 200, 300 and 400 g kg À1 RSM replacing graded levels of SBM respectively. Each diet was randomly assigned to triplicate groups of 30 fish (initial average weight 5.2 g) per aquarium in a rearing system maintained at 29 AE 1°C for 8 weeks. The hepatic protease and lipase activities gradually decreased with increasing dietary RSM level, but no significant differences were observed among the low inclusion level (0-200 g kg À1 ) groups. The apparent digestibility coefficients of dry matter, crude protein, crude lipid and ash showed a similar trend as the hepatic protease and lipase activities. The retentions of protein and individual essential amino acid (except lysine, threonine and leucine) in fish fed diet with 200 g kg À1 RSM were similar to those in fish fed the control diet. These results indicate that dietary RSM inclusion level up to 200 g kg À1 did not markedly affect the digestive enzyme activity, nutrient digestibility and retention in tilapia, whereas these were depressed by the inclusion of 400 g kg À1 RSM.