Assessment of the fecundity, population growth and fatty acid composition of<i>Apocyclops royi</i>(Cyclopoida, Copepoda) fed on different microalgal diets

Pan, Yen‐Ju; Sadovskaya, Irina; Hwang, Jiang‐Shiou; Souissi, Sami

doi:10.1111/anu.12633

Cited by 31 publications

(38 citation statements)

References 61 publications

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“…This was further supported by the smaller relative contents of ALA, SDA and EPA found in RHO and TET, indicating that the biosynthetic process was progressing. These overall findings support the findings of Pan et al (2017).…”

Section: Discussionsupporting

confidence: 90%

“…Other copepod species are also likely to possess the ability for n-3 LC-PUFA biosynthesis. This ability is suggested to be present in the calanoids Calanus finmarchicus (Bell et al, 2007) and Paracalanus parvus (Moreno et al, 1979), the harpacticoid Tisbe holothuriae (Norsker and Støttrup, 1994), the cyclopoids Eucyclops serrulatus (Desvilettes et al, 1997) and Paracyclopina nana (Lee et al, 2006), and also in the candidate species of the present study A. royi (Pan et al, 2017). These studies used direct comparison of microalgae diet and copepod FA profiles to support their claims, except Bell et al (2007) and Moreno et al (1979) who used another approach and conducted isotope-marking experiments that made it possible to directly follow the progression of the fatty acid bioconversion and gives more substantial proof of biosynthesis.…”

Section: Introductionmentioning

confidence: 50%

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n-3 PUFA biosynthesis by the copepod Apocyclops royi determined by fatty acid profile and gene expression analysis

et al. 2019

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The cyclopoid copepod Apocyclops royi (Lindberg 1940) is one of two dominant mesozooplankton species in brackish Taiwanese aquaculture ponds. Periodically low n-3 polyunsaturated fatty acid (PUFA) content in seston could potentially be a limiting factor for zooplankton diversity. Apocyclops royi ’s potential ability to biosynthesize n-3 PUFA was investigated through a short-term feeding experiment on four species of microalgae . Furthermore, we analyzed the expression of genes encoding putative fatty acid elongase ( ELO ) and desaturase ( FAD ) enzymes in A. royi on long-term diets of the PUFA-poor Dunaliella tertiolecta and the PUFA-rich Isochrysis galbana . The copepods exhibited high contents of docosahexaenoic acid (DHA, C22:6n-3) (>20% of total fatty acid) even when DHA-starved for two generations, and no significant differences were found in absolute DHA content between treatments. Transcripts correlating to the four enzymes Elovl4, Elovl5, Fad Δ5 and Fad Δ6 in the n-3 PUFA biosynthetic pathway were identified. Gene expression analysis revealed a significantly higher expression of two desaturases similar to Fad Δ6 in copepods fed PUFA-lacking algae compared to copepods fed algae with high PUFA content. These findings suggest a highly active n-3 PUFA biosynthesis and capability of DHA production in A. royi when fed low-PUFA diets.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 50%

n-3 PUFA biosynthesis by the copepod Apocyclops royi determined by fatty acid profile and gene expression analysis

et al. 2019

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“…Dietary fatty acid ingested by female copepod could affect the fatty acid of their eggs (Rayner et al., ), and it could be correlated with copepod egg viability (Broglio, Jónasdóttir, Calbet, Jakobsen & Saiz, ). The EPA deficiency in the eggs could be the consequence of low EPA proportion in the microalgal diet I. galbana (Pan et al., ) fed by the maternal A. bilobata . The data collected here provide a preliminary exploration, yet the effects of dietary nutrients on the fatty acid compositions of the eggs and their cold storage capacities have to be investigated with molecular tools in the future.…”

Section: Discussionmentioning

confidence: 99%

“…The upper suspension was transferred and then evaporated completely in new glass vials. Transesterification was carried out following the protocol reported by Pan, Sadovskaya, Hwang and Souissi (). Briefly, the lipid was transesterificated in methanol‐toluene‐acetyl chloride reagent for 2 hr at 95°C, and the fatty acid methyl esters were purified and resolved in hexane for gas chromatography–mass spectrometry (GC–MS) analysis.…”

Section: Methodsmentioning

confidence: 99%

Egg hatching rate and fatty acid composition of Acartia bilobata (Calanoida, Copepoda) across cold storage durations

et al. 2018

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To investigate egg storage capacity of the copepod Acartia bilobata for aquaculture interest, we tested hatching success rate (HSR) of inclusive eggs (mixture of all egg types) after 4°C storage. The HSR peaked after 14 days storage when incubating at 28°C for 48 hr (85.8 ± 1.6%) and 72 hr (87.6 ± 0.9%), then gradually declined until 1 year (48 hr: 7 ± 0.6%; 72 hr: 19.4 ± 3.9%). Reallocation of fatty acid profile suggests that docosahexaenoic acid (DHA) is correlated with the HSR of A. bilobata eggs. Additionally, we investigated the HSR of diapausing eggs (unhatched eggs after 72 hr incubation of the inclusive eggs) after 4°C storage. Their HSR peaked after 14 days storage (48 hr:75.3 ± 3.5%; 72 hr:78.2 ± 2.1%), then gradually declined until 60 days (48 hr HSR:42.1 ± 2.3%; 72 hr HSR:53.0 ± 3.2%). Overall, we illustrated the hatchability of diapausing and quiescent eggs of A. bilobata after 4°C storage. The cold storage capacities were low (<60% HSR after 60 days), and it could be limited by the egg DHA content. Our findings provide implications for future studies aiming to improve cold storage techniques of tropical copepod eggs for aquaculture applications. K E Y W O R D SAcartia bilobata, diapausing egg, egg cold storage, fatty acid, quiescent egg

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“…It has a life cycle of 7-8 days [5], and can tolerate temperatures of 15-35 °C [6], and salinities of 0-35 psu [7]. In a recent publication, we report its ability to biosynthesize the polyunsaturated fatty acid Docosahexaenoic acid (DHA) from alpha -Linolenic acid [8, 9] which makes Apocyclops royi an interesting organism for copepod physiological studies.…”

Section: Introductionmentioning

confidence: 99%

The whole genome sequence and mRNA transcriptome of the tropical cyclopoid copepod Apocyclops royi

Jørgensen

Nielsen

Petersen

et al. 2018

Preprint

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Copepoda is one of the most ecologically important animal groups on Earth, yet very few genetic resources are available for this Subclass. Here, we present the first whole genome sequence (WGS, acc. UYDY01) and the first mRNA transcriptome assembly (TSA, Acc. GHAJ01) for the tropical cyclopoid copepod species Apocyclops royi. Until now, only the 18S small subunit of ribosomal RNA gene and the COI gene has been available from A. royi, and only one other cyclopoid copepod had WGS resources available. Overall, the provided resources are the 7th copepod species to have WGS available and the 19th copepod species with TSA information available. We analyze the length and GC content of the provided WGS scaffolds as well as the coverage and gene content of both the WGS and the TSA assembly. Finally, we place the resources within the copepod order Cyclopoida as a member of the Apocyclops genus. We estimate the total genome size of A. royi to 450 Mb, with 181 Mb assembled nonrepetitive, 76 Mb assembled repeats and 193Mb unassembled sequence. The TSA assembly consists of 29,737 genes and an additional 45,756 isoforms. In the WGS and TSA assemblies, >80% and >95% of core genes can be found, though many in fragmented versions. The provided resources will allow researchers to conduct physiological experiments on A. royi, and also increase the possibilities for copepod gene set analysis, as it adds substantially to the copepod datasets available.

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Assessment of the fecundity, population growth and fatty acid composition ofApocyclops royi(Cyclopoida, Copepoda) fed on different microalgal diets

Cited by 31 publications

References 61 publications

n-3 PUFA biosynthesis by the copepod Apocyclops royi determined by fatty acid profile and gene expression analysis

n-3 PUFA biosynthesis by the copepod Apocyclops royi determined by fatty acid profile and gene expression analysis

Egg hatching rate and fatty acid composition of Acartia bilobata (Calanoida, Copepoda) across cold storage durations

The whole genome sequence and mRNA transcriptome of the tropical cyclopoid copepod Apocyclops royi

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