Assessment of the frequency of mitochondrial tRNA variants in patients with ovarian cancer

Abstract

Background: Alternations in mitochondrial DNA (mtDNA) have long been proposed to be involved in the pathogenesis of ovarian cancer (OC). However, the allele frequency of mitochondrial tRNA (mttRNA) variants in OC remains largely understood. Objective: To explore the potential association between mt-tRNA variants and OC. Methods: 95 OC patients and 50 control subjects were participated in this study. The 22 mt-tRNA genes were PCR amplified and subsequently sequenced by ABI-3700 automatic DNA sequencer. Moreover, we used phylogenetic approach to determine the pathogenic status of these mt-tRNA variants. Results: We identified 4 mt-tRNA variants: the tRNA Thr A15951G; tRNA His G12192A; tRNA Glu A14693G and tRNA Leu(UUR) A3302G, whereas these tRNA variants were absent in healthy controls. Moreover, these variants localized at positions which were highly conserved between different species, and may cause the failure in mt-tRNAs metabolism, consequently result in mitochondrial dysfunction that responsible for OC. Conclusions: Variations in mt-tRNAs may be associated with OC.

Design and research of electromagnetic repulsion mechanism with double repulsion disks structure

“…High-voltage DC power transmission has a longer transmission distance and higher transmission efficiency, compared with high-voltage AC power transmission, which provides solutions for new energy grid connections and realizes optimal allocation of energy resources [1][2][3] . The development of the DC power grid requires reliable DC breakers, Hybrid circuit breakers that use fast switching.…”