Assessment of the function of gonad-specific PmAgo4 in viral replication and spermatogenesis in Penaeus monodon

Ho, Teerapong; Panyim, Sakol; Udomkit, Apinunt

doi:10.1016/j.dci.2020.103824

Cited by 5 publications

(4 citation statements)

References 67 publications

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“…A knockdown study of this gene in P . monodon [ 38 ] using RNAi found that reducing this gene in transcript level resulted in testicular maturity and a decrease in the number of spermatogonia in the spermatophore, revealing the critical role in controlling spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…AGO4 encodes a protein that contains PAZ and PIWI domains and plays gene regulation via RNA interference and short-interfering-RNA-mediated gene silencing [37]. A knockdown study of this gene in P. monodon [38] using RNAi found that reducing this gene in transcript level resulted in testicular maturity and a decrease in the number of spermatogonia in the spermatophore, revealing the critical role in controlling spermatogenesis.…”

Section: Plos Onementioning

confidence: 99%

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Differentially expressed genes in the testes from early to mature development of banana shrimp (Fenneropenaeus merguiensis)

Saetan,

Chotigeat

2023

PLoS ONE

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Banana shrimp (Fenneropenaeus merguiensis) is an economically important species in Thailand owing to the high value of globally exported frozen brine shrimps. However, the regulatory mechanisms governing spermatogenesis and testicular development in this species are poorly understood. High-throughput RNA sequencing was used to investigate the mechanisms and regulated genes involved in testis development using transcriptome profiling of juvenile and adult banana shrimp testes. Differentially expressed genes (DEGs) in these two libraries were identified and quantified to confirm gene expression. DEGs were found in 7,347 genes, with 4,465 upregulated and 2,882 downregulated. Some of these genes were designated as candidate genes, and six specific DEGs, including PRM1, SPATA20, Sry, SSRF, Sxl, and Tra-2c, were selected to confirm the reliability of the RNA-seq data using qPCR. Moreover, six non-DEGs were chosen based on testis-specific and regulatory genes that support a specific function in spermatogenesis and testis development in this species, including Dsx, Gfra2, IAG, Sox9, Sox13, and Sox14A. Furthermore, Sry, Sox14A, Sox14B and SPATA20 were identified in early stages (nauplius-postlarvae) of shrimp development to provide more information involving testes formation and development. The transcript data from this study could differentiate a group of genes required at the early and late stages of testis development and both sets of testis development. Therefore, this information would help in manipulating each stage of testicular development.

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Section: Discussionmentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Differentially expressed genes in the testes from early to mature development of banana shrimp (Fenneropenaeus merguiensis)

Saetan,

Chotigeat

2023

PLoS ONE

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“…15−17 Likewise, Ago1 and Ago3 genes are involved in dsRNA-mediated gene silencing pathway in shrimps; 18 and Ago4 showed irrelevant association with RNAi-mediated gene silencing and defense against viral infection. 19 In this context, RNAi machinery seems to primarily mediate gene regulation through miRNAs in mammals. 12 It has been found that the siRNAs are fully paired to a specific, complementary sequence of their target mRNA, while micro-RNAs (miRNAs) are not required to fully match with their target mRNA.…”

Section: ■ Introductionmentioning

confidence: 99%

“…However, RNAi machinery is functional and seemed to be a backline defense in case of any defect in the IFN system in vertebrates. , In addition, mammals have a single Dicer that participates in the generation of both siRNAs and miRNAs and have four Ago genes that are responsible for microRNAs (miRNAs)-guided gene silencing, and Ago2 only mediates siRNA-dependent RNAi . In contrast, in invertebrates, such as shrimps, there are more than one Dicer gene with different functional activities in RNAi machinery. − Likewise, Ago1 and Ago3 genes are involved in dsRNA-mediated gene silencing pathway in shrimps; and Ago4 showed irrelevant association with RNAi-mediated gene silencing and defense against viral infection . In this context, RNAi machinery seems to primarily mediate gene regulation through miRNAs in mammals …”

Section: Introductionmentioning

confidence: 99%

RNA Interference in Aquaculture: A Small Tool for Big Potential

Abo‐Al‐Ela

2021

J. Agric. Food Chem.

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For decades, the tight regulatory functions of DNA and RNA have been the focus of extensive research with the goal of harnessing RNA molecules (e.g., microRNA and small interfering RNA) to control gene expression and to study biological functions. RNA interference (RNAi) has shown evidence of mediating gene expression, has been utilized to study functional genomics, and recently has potential in therapeutic agents. RNAi is a natural mechanism and a well-studied tool that can be used to silence specific genes. This method is also used in aquaculture as a research tool and to enhance immune responses. RNAi methods do have their limitations (e.g., immune triggering); efficient and easy-to-use RNAi methods for large-scale applications need further development. Despite these limitations, RNAi methods have been successfully used in aquaculture, in particular shrimp. This review discusses the uses of RNAi in aquaculture, such as immune- and production-related issues and the possible limitations that may hinder the application of RNAi in the aquaculture industry. Our challenge is to develop a highly potent in vivo RNAi delivery platform that could complete the desired action with minimal side effects and which can be applied on a large-scale with relatively little expense in the aquaculture industry.

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Ago4-piRNA complex is a key component of genomic immune system against transposon expression in Penaeus monodon

Ho,

Eichner,

Sathapondecha

et al. 2024

Fish & Shellfish Immunology

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Assessment of the function of gonad-specific PmAgo4 in viral replication and spermatogenesis in Penaeus monodon

Cited by 5 publications

References 67 publications

Differentially expressed genes in the testes from early to mature development of banana shrimp (Fenneropenaeus merguiensis)

Differentially expressed genes in the testes from early to mature development of banana shrimp (Fenneropenaeus merguiensis)

RNA Interference in Aquaculture: A Small Tool for Big Potential

Ago4-piRNA complex is a key component of genomic immune system against transposon expression in Penaeus monodon

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