Assessment of the functional efficacy of root canal treatment with high-frequency waves in rats

Abstract

The purpose of this study was to develop a high-frequency wave therapy model in rats and to investigate the influence of high-frequency waves on root canal treatment, which may provide a novel strategy for treating apical periodontitis. Root canal treatments with and without high-frequency wave irradiation were performed on the mandibular first molars of 10-weekold male Wistar rats. The mesial roots were evaluated radiologically, bacteriologically, and immunohistochemically. At 3 weeks after root canal treatment, lesion volume had decreased significantly more in the irradiated group than in the non-irradiated group, indicating successful development of the high-frequency therapy model. The use of high-frequency waves provided no additional bactericidal effect after root canal treatment. However, highfrequency wave irradiation was found to promote healing of periapical lesions on the host side through increased expression of fibroblast growth factor 2 and transforming growth factor-β1 and could therefore be useful as an adjuvant nonsurgical treatment for apical periodontitis.

Morphological Assessment of the Effect of Nanosecond Diode Laser Radiation With a Wavelength of 1265 nm on Periodontal Tissue in the Treatment of Apical Periodontitis: An Experimental Study

“…1, which promotes healing of periapical lesions and may be useful as an adjuvant non-surgical treatment of apical periodontitis. 26 Morphological studies of the periapical region after treatment with PDT-induced periodontitis in dogs demonstrated a decrease in periapical lesions, a decrease in the number of inflammatory cells, moderate neoangiogenesis and fibrogenesis compared to the group without PDT with root canal treatment in one session. 27 Studies of modern scientists using X-ray and morphological studies show that the use of laser radiation in the treatment of apical periodontitis does not contribute to significant tissue repair, but at the same time contributes to a significant decrease in inflammatory cells, which indicates a significant anti-inflammatory effect of laser radiation and PDT.…”

Comparison of EASYDO ACTIVATOR, passive ultrasonic, and needle irrigation techniques on the treatment of apical periodontitis: a study in rats

“…However, the apical periodontitis model in pigs is very expensive, which may limit its application. Researchers have used rats as experimental apical periodontitis models and assessed the changes in the lesions by different measures, such as hematoxylin and eosin (H&E) staining and micro-CT analysis [ 20 , 21 ]. In these models, root canal treatment (RCT) significantly reduced the bacterial count in the root canal, establishing a system leading to clinical healing.…”