Assessment of the genetic diversity of Kenyan coconut germplasm using simple sequence repeat (SSR) markers

Abstract

Genetic diversity and relationship among 48 coconut individuals (Cocos nucifera L.) collections from the Coastal lowland of Kenya were analyzed using 15 simple sequence repeat (SSR) primer pairs. Diversity parameters were calculated using Popgene Software version 1.31. The gene diversity values ranged from 0.0408 (CAC68) to 0.4861 (CAC23) with a mean value of 0.2839. The polymorphic information content (PIC) values ranged from 0.0400 to 0.3680 with a mean value of 0.2348. Marker CAC23 had the highest PIC and revealed highest gene diversity values in this study. Analysis of the molecular variation indicated that within individual variation was 98% while among materials, variation was low at 2% suggesting that molecular variation was not defined by region of production. Cluster analysis was constructed using DARwin program version 6.0. Forty eight (48) coconut individuals were clustered into three groups.

De Novo Genome Sequence Assembly of Dwarf Coconut (Cocos nucifera L. ‘Catigan Green Dwarf’) Provides Insights into Genomic Variation Between Coconut Types and Related Palm Species

“…Meanwhile. the genome-wide SSR marker would be an invaluable genetic resource for a wide-array of applications in coconut such as assessment of genetic diversity (Oyoo et al 2016), fingerprinting (Preethi et al 2016; Kamaral et al 2017), ecological-genetic studies (Geethanjali, et al 2018), gene flow characterization (Larekeng et al 2015) and fine- mapping genetic studies (Riedel et al 2009). On the other hand, high-throughput and SNP-based marker assays such as microarray and genotyping-by-sequencing can now be applied in coconut breeding.…”