Assessment of the genetic diversity and genetic relationships of pomegranate (Punica granatum L.) in China using RAMP markers

Zhao, Lei; Li, Mingyang; Cai, Guohong; Tian-chun, Pan; Shan, Chen

doi:10.1016/j.scienta.2012.12.015

Cited by 25 publications

(13 citation statements)

References 21 publications

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“…Hence, our results are clearly distinguishing among the local and the international varieties of performance in Egypt along with three separate categories. Apparently, in view with the previous revisions, our results obtained are better than expected, along with the discrimination capacity and the level of polymorphism when compared to the previous studies in Iran, China, Morocco, Oman and Egypt (Moslemi et al, 2010;Alsadi et al, 2012;Zhao et al, 2013;Ajal et al, 2014;Sinjare, 2015;Al-Sadi et al, 2015;Ismail et al, 2014). In contrast with the previous study in Egyptian pomegranate cultivars created on ISSR and AFLP by Ismail et al, (2014).…”

Section: Discussioncontrasting

confidence: 50%

“…Consequently, it is vital to conserve the gene pool alongside with the behavior of genes in pomegranate germplasm, to maintain a broad genetic base for the future aspect of breeding strategy in pomegranate (Rana et al, 2007). Numerous molecular marker investigations have been verified in P. granatum, such as RAPD (Talebi et al, 2003); ISSR (Talebi et al, 2005;Narzary et al, 2009); F-AFLP (Yuan et al, 2007); RFLP (Melgarejo et al, 2009); AFLP (Moslemi et al, 2010); SRAP (Soleimani et al, 2012); SSR (Rania et al, 2012;Hasnaoui et al, 2012); REMAP (Zhao et al, 2013) and chloroplast DNA (Hajiahmadi et al, 2013). In fact, the choice of most appropriate technology for a specific study is not obvious and depends primarily on purpose of the research as well as the degree of polymorphism and genetic assembly of the species.…”

Section: Introductionmentioning

confidence: 99%

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Utilization of ISTR, ISSR and SRAP molecular markers to reveal and classify Egyptian pomegranates (Punica granatum L.)

Amar¹,

El-Zayat²

2017

Plant Omics

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This study offers the comprehensive analysis of the genetic diversity among six local varieties (Manfalouti; Nab El-Gamal; Hegazy; Wardi; Assuity; Araby) and three international varieties (Wonderful; Marked Bani Rafie; Red Bani Rafie) of pomegranate in Egypt, based on the behavior of ISTR, ISSR and SRAP markers profiles. A set of 24 ISTR, 16 ISSR and 30 SRAP primers combination were compared, concerning exposed the degree of resolution and efficiency of the discriminating capacity technique alongside with the level of genetic polymorphism, to determine which varieties evolved from the others. Considering the results, SRAP assay had superior, more sensitive, higher discrimination capacity and gives much more evidence about the total number of effective alleles (1411), number of polymorphic amplicons (312), PIC (0.94), assay efficiency index (47.04), effective multiples ratio (10.04) and marker index (9.74). Unexpectedly, the ISTR profile demonstrated a significant moderate level of polymorphism among tested genotypes. To further determine the genetic relationships and the distance among varieties, a graphic demonstration of combined UPGMA tree and the PCA analysis did assemble with clear resolution and accurate along with three categories.Our findings confirm that combining different marker system were greatly better and more effective considered an important priority step toward diversity study and characterization. We can point out that, the two-widespread varieties in Egypt Manfalouti, and Nab El-Gamal, formed a high homogeneity in genetic similarity with the Wonderful, as an essential global species for breeding programs, efforts widening the genetic base of pomegranates and the introduction of new genotypes in Egypt.

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Section: Discussioncontrasting

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Utilization of ISTR, ISSR and SRAP molecular markers to reveal and classify Egyptian pomegranates (Punica granatum L.)

Amar¹,

El-Zayat²

2017

Plant Omics

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“…RAPD has been successfully applied in genetic variation investigations of Mucuna pruriens due to its usefulness as an informative and cheap tool for plant breeding programs (Patil et al 2016). There are also many researchers using these markers as one of their techniques to study the genetic diversity of their desired plant species (Desai et al 2015;Kumar et al 2009Kumar et al , 2010Mei et al 2015;Patel et al 2015;Patil et al 2016;Pu et al 2009;Saleh 2015;Zhao et al 2013;Zhao et al 2015). RAPD and ISSR are dominant markers that function well in DNA fingerprinting studies of plant species.…”

Section: Introductionmentioning

confidence: 99%

“…RAMP has been shown to be useful in detecting and mapping co-dominant microsatellite polymorphisms without tedious cloning and sequencing (Wu et al 1994). Although the RAMP molecular marker has not been widely tested in other plant species, it is a useful technique because it is suitable for genetic analyses of plants whose genetic background is unclear (Zhao et al 2013). The information on genetic diversity is useful as it serves as a guide on proper growing practice, whether by stem-cutting or sexual reproduction.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of genetic diversity of Clinacanthus nutans (Acanthaceaea) using RAPD, ISSR and RAMP markers

Ismail

Arsad

Samian

et al. 2016

Physiol Mol Biol Plants

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Three polymerase chain reaction (PCR) techniques were compared to analyse the genetic diversity of Clinacanthus nutans eight populations in the northern region of Peninsular Malaysia. The PCR techniques were random amplified polymorphic deoxyribonucleic acids (RAPD), inter-simple sequence repeats (ISSR) and random amplified microsatellite polymorphisms (RAMP). Leaf genomic DNA was PCR amplified using 17 RAPD, 8 ISSR and 136 RAMP primers . However, only 10 RAPD primers, 5 ISSR primers and 37 RAMP primers produced reproducible bands. The results were evaluated for polymorphic information content (PIC), marker index (MI) and resolving power (RP). The RAMP marker was the most useful marker compared to RAPD and ISSR markers because it showed the highest average value of PIC (0.25), MI (11.36) and RP (2.86). The genetic diversity showed a high percentage of polymorphism at the species level compared to the population level. Furthermore, analysis of molecular variance revealed that the genetic diversity was higher within populations, as compared to among populations of C. nutans. From the results, the RAMP technique was recommended for the analysis of genetic diversity of C. nutans.

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“…A number of studies addressed genetic diversity of pomegranate in different parts of the world (Moslemi et al 2010;Alamuti et al 2012;Zhao et al 2013;Ferrara et al 2014). Despite the importance of pomegranate in Oman, there is a lack of studies concerning relationship among Omani pomegranate cultivars and with cultivars from the two centers of origin of Punica spp., Iran and Yemen.…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis suggests a shared origin of Punica granatum cultivars in Oman with cultivars from the center of origin, Iran

Al-Sadi

Al-Fahdi

Al-Yahyai

et al. 2015

Genet Resour Crop Evol

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Pomegranate is important ancient fruit shrub in the Middle East, where it originated and has been cultivated for centuries. Oman, which lies between the two centers of origin of Punica spp. (Yemen and Iran), is known for cultivation of pomegranates. However, little is known about the relationship of pomegranates from Oman to pomegranates in Iran and Yemen. A study was conducted to investigate genetic diversity and relatedness among five cultivars of pomegranate from Oman with nine cultivars from Iran (5), Yemen (1), India (1), Lebanon (1) and Spain (1). A total of 88 pomegranate samples representing 14 cultivars (2-15 samples/cultivar) were analyzed using Amplified Fragment Length Polymorphism. The analysis produced 889 polymorphic loci and 88 genotypes. Omani, Yemeni and some Iranian cultivars clustered together, providing evidence for a relationship of clustering with geographical origin. AMOVA analysis indicated the existence of moderate and significant levels of genetic differentiation among all pomegranate cultivars (F ST = 0.133), which indicates moderate level of cultivar exchange among countries. However, AMOVA analysis and Nei genetic similarity levels provided evidence that one cultivar each from Oman, Iran and Yemen, one from Oman and Iran and one from India and Spain were found to have a high level of genetic similarity. This suggests that these cultivars may have a common origin and have been moved among these countries via trade routes in the past. The findings are discussed in terms of factors affecting diversity of pomegranate.

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Assessment of the genetic diversity and genetic relationships of pomegranate (Punica granatum L.) in China using RAMP markers

Cited by 25 publications

References 21 publications

Utilization of ISTR, ISSR and SRAP molecular markers to reveal and classify Egyptian pomegranates (Punica granatum L.)

Utilization of ISTR, ISSR and SRAP molecular markers to reveal and classify Egyptian pomegranates (Punica granatum L.)

Evaluation of genetic diversity of Clinacanthus nutans (Acanthaceaea) using RAPD, ISSR and RAMP markers

Genetic analysis suggests a shared origin of Punica granatum cultivars in Oman with cultivars from the center of origin, Iran

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