Assessment of the genotoxic and mutagenic properties of Himatanthus articulatus bark extracts used as phytotherapeutic drug in the Amazon

Rebouças, Sebastião de O.; Silva, Juliana da; Bertoni, Ricardo S.; Decker, Natália; Santos, Marcelo Soares Teles; Rossatto, Raíssa Rebés; Corrêa, Dione Silva; Ferraz, Alexandre de Barros Falcão

doi:10.1016/j.jep.2013.03.041

Cited by 12 publications

(4 citation statements)

References 43 publications

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“…In all the experiments, the comparisons between controls and treated groups were made by one-way ANOVA and post hoc test (DMR, Dunnett's multiple comparison test) using SPSS 12.0. Data with P < 0.05 were considered significant (Rebouças et al, 2013;Eke and Çelik, 2008).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic and genotoxic action of Tagetes patula flower methanol extract and patuletin using the Allium test

et al. 2019

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Tagetes patula is used to treat cancer patients in alternative healthcare systems. However, its cytotoxic and genotoxic effects have not been reported. Therefore, themethanol extract of T. patula flower, the ethyl acetate fraction, and the pure compound patuletin were evaluatedusing the Allium test.The methanol extract and fraction contained ~3% and ~36% patuletin, respectively, with ~98% purity. The methanol extract caused inhibition of Allium root growth displaying an IC50 value of ~500 µg/mL, while the fraction and patuletin were more potent by ~2 and ~5 times, respectively. The Allium root tips demonstrated a decline in prophase, metaphase, anaphase, and telophase stages with concomitant decrease in percent mitotic index in the methanol extract (~5.64), fraction, and patuletin (~4) as compared to the control (~7.61). However, in only methanol extract-treated root tips, an increase in metaphase stage was noted. In addition, the methanol extract predominantly induced c-type, misaligned, and multipolar chromosomal abnormalities while the fraction and patuletin displayed fragments and sticky chromosomes. The fraction and patuletin also produced micronuclei (~2%). In conclusion, T. patula flower methanol extract and ethyl acetate fraction are cytotoxicand genotoxic, which most likely could be due to the patuletin. Further preclinical and clinical studies are required to justify its clinical use.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic and genotoxic action of Tagetes patula flower methanol extract and patuletin using the Allium test

et al. 2019

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“…Quanto ao ensaio em organismos marinhos, com objetivo de avaliar a toxicidade de substâncias com potencial farmacológico, os órgãos fiscalizadores sugerem que sejam utilizadas mais de uma espécie e que pertençam a diferentes níveis tróficos. Assim, geralmente são utilizadas algas (base da cadeia alimentar), crustáceos (consumidores primários das algas), e os peixes, que são os consumidores secundários (42) .…”

Section: As Moléculas De Isômerosunclassified

Estudo in silico das atividades de triterpenos e iridoides isolados de Himatanthus articulatus (Vahl) Woodson

Dolabela

Silva

Ohashi

et al. 2018

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O presente trabalho realizou estudos in silico para predição das atividades farmacocinética, toxicológica e biológica de triterpenos e iridoides isolados de Himatanthus articulatus, utilizada popularmente contra malária, afecções na pele, helmintíases, úlceras gástricas, gastrites, tumores e sífilis. As comparações foram realizadas através dos programas: ChemSketch, PreADMET e PASS online. As características físico-químicas dos compostos foram estimadas pelos servidores online Mcule property calculator e Chemicalize.org. Os resultados revelaram que os triterpenos e os iridoides não glicosilados (plumericina e isoplumericina) apresentaram absorção intestinal acima de 90%. Apenas a plumericina e a isoplumericina seguem a regra de Lipinsk, além de apresentarem atividade antineoplásica, antibacteriana e antifúngica. O cinamato de lupeol, cinamato de α-amirina e acetato de lupeol provavelmente atuam nos sinais e sintomas da malária, porém, o acetato de lupeol apresentou maior número de reações adversas. Todas as substâncias mostraram carcinogenicidade, mas apenas os triterpenos demonstraram mutagenicidade. Em termos farmacocinético, toxicológico e biológico, apenas a plumericina e a isoplumericina apresentaram resultados satisfatórios.

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“…Plumieride ( Figure 1 ) is an iridoid, isolated as a major constituent from the extract of Plumeria bicolor stem bark [ 6 , 7 ]. Further, in vitro anticancer potential and various pharmacological activities of plumieride have been conducted on mammals [ 8 ] whereas its antioxidant potential through possible mechanisms of action remains uninvestigated [ 9 , 10 , 11 , 12 , 13 ]. These medicinal properties and lack of references motivated us to investigate the antioxidant potential of plumieride from Plumeria bicolor .…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Potential of Plumieride against CCl4-Induced Peroxidative Damage in Rats

et al. 2014

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In search of a new potent as an antioxidant from natural sources, plumieride—an iridoid isolated from the methanol extract of the bark of Plumeria bicolor (family Apocynaceae) was evaluated for its antioxidant potential against CCl4-induced peroxidative damage in liver of rats. The antioxidant potential was evaluated by using hepatic tissue for SOD (superoxide dismutase), CAT (catalase), GSH (reduced glutathione), GPx (glutathione peroxidase), GR (glutathione reductase) and LPO (lipid peroxidation) alongwith the concomitant blood serum for AST & ALT (aspartate and alanine transaminases), GGT (gamma glutamyl transpeptidase), ALP (alkaline phosphatase), total bilirubin and total protein contents. All the biochemical parameters were significantly (p ≤ 0.001) altered by CCl4 (0.3 mL/kg body weight/twice a week, intra-peritoneally for 30 days). Simultaneously, oral treatment with plumieride (5, 10 and 20 mg/kg body weight/day for 30 days), restored all the parameters towards a normal level, remarkably. The histological findings of liver sections further corroborated the antioxidant potential of plumieride compared with standard drug-silymarin. In conclusion, plumieride consists of sugar molecules, which have alcoholic groups. Therefore, the alcoholic groups of sugar increase its antioxidant potential through intermolecular hydrogen bonding along with the thiol(SH) group of non-protein thiols and enzymes resulting in the restoration of the antioxidant system. Therefore, it might be considered a natural antioxidant against peroxidative damage in rats.

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Assessment of the genotoxic and mutagenic properties of Himatanthus articulatus bark extracts used as phytotherapeutic drug in the Amazon

Abstract: The toxicological evaluation indicated that the extracts are non-genotoxic and reduce the clastogenic damage induced by hydrogen peroxide. In part, this result can be atributted to the phytochemical profile of Himatanthus articulatus, which presents iridoids and phenolic compounds.

Cited by 12 publications

References 43 publications

Cytotoxic and genotoxic action of Tagetes patula flower methanol extract and patuletin using the Allium test

Cytotoxic and genotoxic action of Tagetes patula flower methanol extract and patuletin using the Allium test

Estudo in silico das atividades de triterpenos e iridoides isolados de Himatanthus articulatus (Vahl) Woodson

Antioxidant Potential of Plumieride against CCl4-Induced Peroxidative Damage in Rats

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