Assessment of the Glycan-Binding Profile of Pseudomonas aeruginosa PAO1

Abstract

P. aeruginosa cells bind to a variety of glycans as part of their interaction with host cells, and a number of P. aeruginosa -encoded receptors and target ligands have been described that allow this microbe to bind to such glycans. Here, we extend this work by studying the glycans used by P. aeruginosa PAO1 cells to bind to phagocytic cells and by using a glycan array to characterize the suite of such molecules that can facilitate host cell binding by this microbe.

Bacteriophages and their unique components provide limitless resources for exploitation

“…Incubation with a cocktail of phages, each displaying a different carbohydrate structure, allows investigators to characterize glycan binding preferences of purified proteins, or glycan recognition by adhesins on specific cell types, or glycan enrichment correlated with known organ functions in mouse models (Sojitra et al, 2021 ; Lin et al, 2023 ). Another application of this method would be to investigate bacterial binding to various glycan structures which very few laboratories have been able to assess through the use of standard glycan arrays (Day et al, 2016 ; Semchenko et al, 2019 ; Sanchez et al, 2023 ).…”

“…able to assess through the use of standard glycan arrays (Day et al, 2016;Semchenko et al, 2019;Sanchez et al, 2023).…”