Assessment of the hybrid status of some Malesian plants using Amplified Fragment Length Polymorphism

Kiew, Ruth; Teo, L; Gan, Y

doi:10.7751/telopea20035617

Cited by 16 publications

(5 citation statements)

References 6 publications

(7 reference statements)

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“…"Kweni", supported by a bootstrap value of 83%, was seen with the diversity analysis using all 36 microsatellite markers. Ding Hou [57] suggested a hybrid origin for M. odorata, which was later verified as a cross between M. indica and M. foetida [58,59]. Based on phylogenetic relationships of the internal transcribed spacer (ITS) sequences of these species, M. odorata is more closely related to M. foetida than to M. indica [60].…”

Section: Discussionmentioning

confidence: 99%

Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) Marker Resources for Diversity Analysis of Mango (Mangifera indica L.)

et al. 2014

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In this study, a collection of 24,840 expressed sequence tags (ESTs) generated from five mango (Mangifera indica L.) cDNA libraries was mined for EST-based simple sequence repeat (SSR) markers. Over 1,000 ESTs with SSR motifs were detected from more than 24,000 EST sequences with di-and tri-nucleotide repeat motifs the most abundant. Of these, 25 EST-SSRs in genes involved in plant development, stress response, and fruit color and flavor development pathways were selected, developed into PCR markers and characterized in a population of 32 mango selections including M. indica varieties, and related Mangifera species. Twenty-four of the 25 EST-SSR markers exhibited polymorphisms, identifying a total of 86 alleles with an average of 5.38 alleles per locus, OPEN ACCESSDiversity 2014, 6 73 and distinguished between all Mangifera selections. Private alleles were identified for Mangifera species. These newly developed EST-SSR markers enhance the current 11 SSR mango genetic identity panel utilized by the Australian Mango Breeding Program. The current panel has been used to identify progeny and parents for selection and the application of this extended panel will further improve and help to design mango hybridization strategies for increased breeding efficiency.

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Section: Discussionmentioning

confidence: 99%

Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) Marker Resources for Diversity Analysis of Mango (Mangifera indica L.)

et al. 2014

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“…This seems to be the case in the zone of sympatry between B. heracleifolia and B. sericoneura, where backcrosses do form despite F1 hybrid sterility. The other documented case of introgression in Begonia is between the closely related South East Asian species B. decora and B. venusta, where a range of intermediate morphologies and admixed AFLP profiles are present in a naturally mixed population (Teo and Kiew, 1999;Kiew et al 2003). The potential for introgression in Begonia is also supported by incongruence of phylogenies constructed with different marker loci (Goodall-Copestake et al 2010).…”

Section: Outcomes Of Natural Hybridization In Begoniamentioning

confidence: 99%

Maintenance of species boundaries in a Neotropical radiation of Begonia

2015

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A major goal of evolutionary biology is to determine the mechanisms generating biodiversity. In Begonia, one of the largest plant genera (1900+ species), it has been postulated that the high number of endemic species is a by-product of low gene flow among populations, which predisposes the group to speciation. However, this model of divergence requires that reproductive barriers accumulate rapidly among diverging species that overlap in their geographic ranges, otherwise speciation will be opposed by homogenizing gene flow in zones of secondary contact. Here, we test the outcomes of secondary contact in Begonia by genotyping multiple sympatric sites with 12 nuclear and seven plastid loci. We show that three sites of secondary contact between B. heracleifolia and B. nelumbiifolia are highly structured, mostly containing parental genotypes, with few F1 hybrids. A sympatric site between B. heracleifolia and B. sericoneura contains a higher proportion of F1s, but little evidence of introgression. The lack of later-generation hybrids contrasts with that documented in many other plant taxa, where introgression is extensive. Our results, in conjunction with previous genetic work, show that Begonia demonstrate properties making them exceptionally prone to speciation, at multiple stages along the divergence continuum. Not only are populations weakly connected by gene flow, promoting allopatric speciation, but species often show strong reproductive barriers in secondary contact. Whether similar mechanisms contribute to diversification in other large genera remains to be tested.

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“…Liquid chromatography-mass spectrometry (LC-MS) is used to classify and compare chemical profiles between N. × hookeriana, a natural hybrid with its parents, N. ampullaria and N. rafflesiana. The parentage of N. × hookeriana was previously discovered based on its morphological resemblance to N. ampullaria and N. rafflesiana (Danser, 1928), which was confirmed by molecular markers, including amplified fragment length polymorphism (AFLP), random amplified polymorphic DNA (RAPD), and inter-simple sequence repeats (ISSR) (Kiew et al, 2003;Yulita and Mansur, 2012). Differences in the morphology and diet between N. ampullaria and N. rafflesiana may lead to differences in the metabolite compositions.…”

Section: Introductionmentioning

confidence: 97%

UPLC-TOF-MS/MS-Based Metabolomics Analysis Reveals Species-Specific Metabolite Compositions in Pitchers of Nepenthes ampullaria, Nepenthes rafflesiana, and Their Hybrid Nepenthes × hookeriana

et al. 2021

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Hybridization is key to the evolution and diversity of plants in nature. Nepenthaceae comprises a family of diverse tropical carnivorous pitcher plant species with extensive hybridization. However, there is no study to date on the metabolite expression of hybrids in this family. We performed a non-targeted metabolomics analysis of the pitchers of two Nepenthes species with different dietary habits, namely, the semi-detritivorous N. ampullaria and carnivorous N. rafflesiana with their hybrid (N. × hookeriana) for a comparative study. The whole-pitcher samples were extracted in methanol:chloroform:water (3:1:1) via sonication-assisted extraction and analyzed using ultra-performance liquid chromatography time-of-flight mass spectrometry (UPLC-TOF-MS) followed by data analysis to profile chemical compositions. A total of 1,441 metabolite features were profiled from the three species in which 43.3% of features in the hybrid samples were not found in either of its parents. The partial least squares discriminant analysis (PLS-DA) found 324 metabolite features with variable in projection (VIP) values greater than one in which 55 features were statistically significant. This showed that the hybrid is closer to N. rafflesiana, which is consistent to the previous study on gene and protein expressions. A total of 105 metabolites were putatively identified with manual searches using public metabolite databases. Phenols were detected to be the most abundant secondary metabolites due to a high flavonoid content, especially in N. rafflesiana. The most abundant feature 476.3s:449.102 was found to be the most significant VIP for distinguishing between the three species as a chemical marker. This is the first study comparing metabolites in the carnivory organs of different Nepenthes species with comprehensive profiling and putative identification. The differential metabolite compositions in the pitchers of different species might have ecological implications with the hybrid showing intermediate phenotype between the parents as well as manifesting unique metabolites. However, there is no clear evidence of metabolites related to the differences in dietary habits between the hybrid and the two parent species.

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Assessment of the hybrid status of some Malesian plants using Amplified Fragment Length Polymorphism

Cited by 16 publications

References 6 publications

Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) Marker Resources for Diversity Analysis of Mango (Mangifera indica L.)

Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) Marker Resources for Diversity Analysis of Mango (Mangifera indica L.)

Maintenance of species boundaries in a Neotropical radiation of Begonia

UPLC-TOF-MS/MS-Based Metabolomics Analysis Reveals Species-Specific Metabolite Compositions in Pitchers of Nepenthes ampullaria, Nepenthes rafflesiana, and Their Hybrid Nepenthes × hookeriana

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