Assessment of the <i>in vitro</i> dermal irritation potential of cerium, silver, and titanium nanoparticles in a human skin equivalent model

Miyani, Vivek A; Hughes, Michael F.

doi:10.1080/15569527.2016.1211671

Cited by 25 publications

(15 citation statements)

References 34 publications

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“…This difference in sensitivity between 2D cell culture and 3D RHE model is consistent with the efficient barrier protection of the reconstructed epidermis. In a recent study [ 36 ], silver nanoparticles with different sizes (10–100 nm) and with different coating (citrate, PVP or silica) as well as silver nitrate similarly did not induce skin irritation. In this study, the exposure time was 1 h followed by 42 h recovery and silver concentration was 1 mg/mL.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models

et al. 2018

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Silver nanowires (AgNW) are attractive materials that are anticipated to be incorporated into numerous consumer products such as textiles, touchscreen display, and medical devices that could be in direct contact with skin. There are very few studies on the cellular toxicity of AgNW and no studies that have specifically evaluated the potential toxicity from dermal exposure. To address this question, we investigated the dermal toxicity after acute exposure of polymer-coated AgNW with two sizes using two models, human primary keratinocytes and human reconstructed epidermis. In keratinocytes, AgNW are rapidly and massively internalized inside cells leading to dose-dependent cytotoxicity that was not due to Ag+ release. Analysing our data with different dose metrics, we propose that the number of NW is the most appropriate dose-metric for studies of AgNW toxicity. In reconstructed epidermis, the results of a standard in vitro skin irritation assay classified AgNW as non-irritant to skin and we found no evidence of penetration into the deeper layer of the epidermis. The findings show that healthy and intact epidermis provides an effective barrier for AgNW, although the study does not address potential transport through follicles or injured skin. The combined cell and tissue model approach used here is likely to provide an important methodology for assessing the risks for skin exposure to AgNW from consumer products.

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Section: Resultsmentioning

confidence: 99%

In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models

et al. 2018

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“…After duplicates removal, 31 studies had their titles and abstracts screened, and 24 studies were excluded because they did not meet the eligibility criteria. Then, the full-text screening did not exclude any studies, resulting in 7 included articles (Park et al, 2011;Choi et al, 2014;Kato et al, 2014;Horie et al, 2016;Kim et al, 2016;Miyani and Hughes, 2016;Tang et al, 2018) in this systematic review (Figure 3 Prisma Flow diagram).…”

Section: Searchmentioning

confidence: 99%

“…From the seven articles found and analyzed, four studies used EpiDerm TM (MatTek Corporation) (Park et al, 2011;Horie et al, 2016;Kim et al, 2016;Miyani and Hughes, 2016), one used the EpiKutis TM (Biocell Biotechnology, China) (Tang et al, 2018), one used KeraSkin (Modern Cell & Tissue Technology, Seoul, Korea) (Choi et al, 2014) and the last one was developed in their own laboratory (Kato et al, 2014). According to the inclusion criteria, all seven articles used TiO 2 NPs as test substance.…”

Section: Study Characteristicsmentioning

confidence: 99%

“…However, some studies also use mixtures of anatase and rutile structures. From the selected articles, two articles used the rutile crystalline structures (Choi et al, 2014;Kato et al, 2014), two articles used the mixture (anatase and rutile) (Park et al, 2011;Kim et al, 2016), one article used two crystalline structures, anatase and rutile (Horie et al, 2016), and two articles used anatase, rutile, and mixture (Miyani and Hughes, 2016;Tang et al, 2018). Some authors have also used other test substances such as: NPs of zinc oxide, zinc oxide/titanium dioxide (Choi et al, 2014), silver (Kim et al, 2016;Miyani and Hughes, 2016), cerium dioxide (Miyani and Hughes, 2016), iron oxide (Kim et al, 2016), aluminum oxide (Kim et al, 2016), polystyrene (Park et al, 2011) and polyvinylpyrrolidone-entrapped fullerene-C 60 (Kato et al, 2014).…”

Section: Study Characteristicsmentioning

confidence: 99%

“…Due to their nano dimensions, they can effectively have electrical, thermal, and mechanical features, desirable for several applications (Davis et al, 2010;Louro et al, 2019;Lüderwald et al, 2019). Consequently, the human exposure to nanoparticles (NPs) increased as a result of their use in industries such as: food, pharmaceutical, cosmetic, biomedical (medical devices: implants, prostheses, controlled drug delivery systems), aeronautics, textiles as well as environmental engineering (Kongsong et al, 2014;Sethi et al, 2014;Semenzin et al, 2015;Miyani and Hughes, 2016;Hanawa, 2019;Shetti et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Toxicity Evaluation of TiO2 Nanoparticles on the 3D Skin Model: A Systematic Review

Sanches

Geaquinto

Cruz

et al. 2020

Front. Bioeng. Biotechnol.

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Titanium dioxide nanoparticles (TiO 2 NPs) are regularly used in sunscreens because of their photoprotective capacity. The advantage of using TiO 2 on the nanometer scale is due to its transparency and better UV blocking efficiency. Due to the greater surface area/volume ratio, NPs become more (bio)-reactive giving rise to concerns about their potential toxicity. To evaluate the irritation and corrosion of cosmetics, 3D skin models have been used as an alternative method to animal experimentation. However, it is not known if this model is appropriate to study skin irritation, corrosion and phototoxicity of nanomaterials such as TiO 2 NPs. This systematic review (SR) proposed the following question: Can the toxicity of TiO 2 nanoparticles be evaluated in a 3D skin model? This SR was conducted according to the Preliminary Report on Systematic Review and Meta-Analysis (PRISMA). The protocol was registered in CAMARADES and the ToxRTool evaluation was performed in order to increase the quality and transparency of this search. In this SR, 7 articles were selected, and it was concluded that the 3D skin model has shown to be promising to evaluate the toxicity of TiO 2 NPs. However, most studies have used biological assays that have already been described as interfering with these NPs, demonstrating that misinterpretations can be obtained. This review will focus in the possible efforts that should be done in order to avoid interference of NPs with biological assays applied in 3D in vitro culture.

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Titanium Dioxide

Battersby,

Warheit

2023

Patty's Toxicology

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Titanium is a naturally occurring element. The commercially most important titanium compound is pigmentary TiO 2 , whereas nano‐scaled TiO 2 represents only a small amount on the market. Since humans are exposed to TiO 2 at workplace or through consumer products, a thorough evaluation of its potential toxicity has been summarized herein. Dermal absorption of TiO 2 is deemed to be negligible, whereas oral and inhalation absorption has been reported but is assumed to be very low. TiO 2 has been shown to be of low acute oral, dermal, and inhalation toxicity and is considered to be void of skin and eye‐irritating properties. It also does not show any potential for skin and respiratory sensitization. Oral long‐term toxicity studies in rats report no adverse effects, and due to lack of dermal absorption of TiO 2 , it is assumed that dermal exposure is also not a hazard. TiO 2 does not show any primary mutagenicity and is also void of reproductive or developmental toxicity. However, in long‐term inhalation toxicity studies in rats, inflammation, fibroproliferative effects, and lung tumor formation has been observed but only under conditions of excessive particle lung overload. Carcinogenicity under such extreme conditions with complete cessation of lung clearance is considered to not imply a cancer hazard for humans, which is in line with epidemiological studies showing no causative link between TiO 2 particle exposure and cancer risk in humans. However, based on the available animal database, IARC in the past classified TiO 2 as possibly carcinogenic to humans, and the Risk Assessment Committee of ECHA also concluded that TiO 2 warrants a Category 2 classification for carcinogenicity.

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Assessment of the in vitro dermal irritation potential of cerium, silver, and titanium nanoparticles in a human skin equivalent model

Cited by 25 publications

References 34 publications

In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models

In Vitro Dermal Safety Assessment of Silver Nanowires after Acute Exposure: Tissue vs. Cell Models

Toxicity Evaluation of TiO2 Nanoparticles on the 3D Skin Model: A Systematic Review

Titanium Dioxide

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