Assessment of the IFN-β response to four feline caliciviruses: Infection in CRFK cells

Jin, Tian; Zhang, Xiaozhan; Wu, Hongxia; Liu, Chunguo; Liu, Jiasen; Hu, Xiaoliang; Qu, Liandong

doi:10.1016/j.meegid.2015.06.003

Cited by 29 publications

(37 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, the IFN β level induced by 5′PPP-RNA treatment was multifold higher than that of TV infection. Several viruses, including hepatitis C virus, vaccinia virus, Ebola virus, astrovirus, feline calicivirus [20] and influenza virus have developed strategies to target and inhibit distinct steps in the early signalling events that lead to the type I IFN induction, indicating the importance of type I IFNs in the host’s antiviral response [8, 9, 12, 17, 21, 38]. For example, influenza A NS1 can target innate immune receptor RIG-I and its signalling components to suppress host innate immune response [39].…”

Section: Discussionmentioning

confidence: 99%

“…For rotaviruses, both RIG-I and MDA-5 as well as MAVS/IPS-1 and IRF3 receptors are responsible for activating IFN β responses [17]. For murine norovirus, MDA5 is the main receptor whereas IRF3 and NF-kB are responsible for activating the IFN β response after infection with feline calicivirus [18–20]. In this study, we investigated the role of RIG-I and MDA5 in LLC-MK2 cells following TV infection.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Critical role of RIG-I and MDA5 in early and late stages of Tulane virus infection

Chhabra

Ranjan

Cromeans

et al. 2017

Journal of General Virology

View full text Add to dashboard Cite

Human noroviruses are a major cause of acute gastroenteritis worldwide, but the lack of a robust cell culture system or small animal model have hampered a better understanding of innate immunity against these viruses. Tulane virus (TV) is the prototype virus of a tentative new genus, Recovirus, in the family Caliciviridae. Its epidemiology and biological properties most closely resemble human norovirus. The host innate immune response to RNA virus infection primarily involves pathogen-sensing toll-like receptors (TLRs) TLR3 and TLR7 and retinoic acid-inducible gene I-like receptor RIG-I and melanoma differentiation associated gene 5 (MDA5). In this study, by using siRNA knockdown, we report that TV infection in LLC-MK2 cells results in an early [3 h post infection (h p.i.), P<0.05] RIG-I-dependent and type I interferon-mediated antiviral response, whereas an MDA5-mediated antiviral effect was observed at later (12 h p.i.; P<0.05) stages of TV replication. Induction of RIG-I and MDA5 was critical for inhibition of TV replication. Furthermore, pre-activation of the RIG-I/MDA5 pathway prevented TV replication (>900-fold decrease; P<0.05), suggesting that RIG-I and MDA5 ligands could be used to develop novel preventive and therapeutic measures against norovirus.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Critical role of RIG-I and MDA5 in early and late stages of Tulane virus infection

Chhabra

Ranjan

Cromeans

et al. 2017

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…The protocol for virus titration was described in our previous study [14]. Briefly, ten-fold diluted virus stocks were prepared, and cells were inoculated with 0.1 mL of each dilution.…”

Section: Viral Titer Testsmentioning

confidence: 99%

Identification of Inonotus obliquus polysaccharide with broad-spectrum antiviral activity against multi-feline viruses

Jin

Liu

et al. 2017

International Journal of Biological Macromolecules

Self Cite

View full text Add to dashboard Cite

Inonotus obliquus polysaccharides (IOPs) are a potential drug for the prevention and treatment of cancer, cardiopathy, diabetes, AIDs, pancreatitis and other diseases. In this study, we found that IOP can act as a broad-spectrum antiviral drug against feline viruses in the in vitro experiment. Using cell models of feline calicivirus (FCV), we demonstrated that IOP treatment was capable of exhibiting anti-FCV strain F9 activity in cell-based assays and also showed low cytotoxicity. Investigation of the mechanism of action of the compound revealed that IOP treatment induces its inhibitory actions directly on virus particles through blocking viral binding/absorpting. The inhibitory activity against other FCV isolates from China was also identified. More importantly, we found that IOP exhibited broad-spectrum antiviral activity against the feline herpesvirus 1, feline influenza virus H3N2 and H5N6, feline panleukopenia virus and feline infectious peritonitis virus that can contribute to respiratory and gastrointestinal diseases in cats. These findings suggest that IOP may be a potential broad-spectrum antiviral drug against feline viruses.

show abstract

“…Luciferase reporter plasmids for the evaluation of canine IFN-β promoter (IFN-Luc), NF-κB response element (3×PRDII-Luc), IRF3 response element (3×PRDIII/I-Luc), and ISRE promoter (ISRE-Luc) were constructed as previously described [21]. Cells grown in 48-well plates were co-transfected with 0.25 μg of luciferase reporter plasmids and 0.25 μg of various expression plasmids using the GenJet™ In Vitro DNA Transfection Reagent.…”

Section: Reporter Plasmids and Luciferase Assaysmentioning

confidence: 99%

Identification and function analysis of canine stimulator of interferon gene (STING)

Zhang

Zhu

et al. 2017

Microbial Pathogenesis

View full text Add to dashboard Cite

Stimulator of interferon gene (STING) plays an important role in the cyclic GMP-AMP synthase (cGAS)-mediated activation of type I IFN responses. In this study, we identified and cloned canine STING gene. Full-length STING encodes a 375 amino acid product that shares the highest similarity with feline STING. Highest levels of mRNA of canine STING were detected in the spleen and lungs while the lowest levels in the heart and muscle. Analysis of its cellular localization showed that STING is localizes to the endoplasmic reticulum. STING overexpression induced the IFN response via the IRF3 and NF-κB pathways and up-regulated the expression of ISG15 and viperin. However, knockdown of STING did not inhibit the IFN-β response triggered by poly(dA:dT), poly(I:C), or SeV. Finally, overexpression of STING significantly inhibited the replication of canine influenza virus H3N2. Collectively, our findings indicate that STING is involved in the regulation of the IFN-β pathway in canine.

show abstract

Assessment of the IFN-β response to four feline caliciviruses: Infection in CRFK cells

Cited by 29 publications

References 36 publications

Critical role of RIG-I and MDA5 in early and late stages of Tulane virus infection

Critical role of RIG-I and MDA5 in early and late stages of Tulane virus infection

Identification of Inonotus obliquus polysaccharide with broad-spectrum antiviral activity against multi-feline viruses

Identification and function analysis of canine stimulator of interferon gene (STING)

Contact Info

Product

Resources

About