Assessment of the immunocontraceptive effect of a zona pellucida 3 peptide antigen in wild mice

Hardy, Christopher M.; Have, J. F. M. ten; Mobbs, Karen J.; Hinds, Lyn A.

doi:10.1071/rd01112

Cited by 24 publications

(24 citation statements)

References 31 publications

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“…In several studies, high titers of ZP-specific total IgG Abs are associated with infertility in mice immunized with the recombinant ZP3 protein or ZP3 peptides (12,18,39). Consistent with those studies, we also demonstrated that the higher level of total IgG Abs was correlated with infertility in mice in the group co-immunized with chi-(pcD- Lzp3 +pcD- mIL-33 ) (Figure 5).…”

Section: Discussionmentioning

confidence: 99%

Molecular adjuvant interleukin-33 enhances the antifertility effect of Lagurus lagurus zona pellucida 3 DNA vaccine administered by the mucosal route

Kadir

Zhang

2013

Braz J Med Biol Res

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It has been shown that cytokines can act as molecular adjuvant to enhance the immune response induced by DNA vaccines, but it is unknown whether interleukin 33 (IL-33) can enhance the immunocontraceptive effect induced by DNA vaccines. In the present study, we explored the effects of murine IL-33 on infertility induced by Lagurus lagurus zona pellucida 3 (Lzp3) contraceptive DNA vaccine administered by the mucosal route. Plasmid pcD-Lzp3 and plasmid pcD-mIL-33 were encapsulated with chitosan to generate the nanoparticle chi-(pcD-Lzp3+pcD-mIL-33) as the DNA vaccine. Sixty female ICR mice, divided into 5 groups (n=12/group), were intranasally immunized on days 0, 14, 28, and 42. After intranasal immunization, the anti-LZP3-specific IgG in serum and IgA in vaginal secretions and feces were determined by ELISA. The results showed that chi-(pcD-Lzp3+pcD-mIL-33) co-immunization induced the highest levels of serum IgG, secreted mucosal IgA, and T cell proliferation. Importantly, mice co-immunized with chi-(pcD-Lzp3+pcD-mIL-33) had the lowest birth rate and mean litter size, which correlated with high levels of antibodies. Ovaries from infertile female mice co-immunized with chi-(pcD-Lzp3+pcD-mIL-33) showed abnormal development of ovarian follicles, indicated by atretic follicles and loss of oocytes. Our results demonstrated that intranasal delivery of the molecular adjuvant mIL-33 with chi-pcD-Lzp3 significantly increased infertility by enhancing both systemic and mucosal immune responses. Therefore, chi-(pcD-Lzp3+pcD-mIL-33) co-immunization could be a strategy for controlling the population of wild animal pests.

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Section: Discussionmentioning

confidence: 99%

Molecular adjuvant interleukin-33 enhances the antifertility effect of Lagurus lagurus zona pellucida 3 DNA vaccine administered by the mucosal route

Kadir

Zhang

2013

Braz J Med Biol Res

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“…One interesting application has been for reproductive control by immunizing animals against sex hormones, gametes or other key targets in the reproductive tract [Figure 1]. [38–42] The most studied reproductive hormone as a vaccine target is luteinizing hormone releasing hormone (LHRH), also known as gonadotrophin-releasing hormone (GnRH). [43–52] GnRH is a small 10 amino acid peptide released from the hypothalamus in all species of mammals and has a central regulatory role in reproductive functions.…”

Section: Types Of Animal Vaccinesmentioning

confidence: 99%

“…Strategies to control fertility using vaccines based on gamete antigens, particularly zona pellucida proteins, have been the most widely researched. [9124253–55] However, commercial successes for immunocontraceptive vaccines have been relatively few primarily because of poor immunogenicity of small peptide self antigens and difficulties in cost-effective practical delivery of the vaccines to target wildlife species. Another interesting evolution which took place with the use of acellular antigens is development of combined vaccines incorporating two or more antigens, initially used for humans and which later paved the way for numerous combined veterinary vaccines.…”

Section: Types Of Animal Vaccinesmentioning

confidence: 99%

Formulation and delivery of vaccines: Ongoing challenges for animal management

Sharma

Hinds

2012

J Pharm Bioall Sci

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Development of a commercially successful animal vaccine is not only influenced by various immunological factors, such as type of antigen but also by formulation and delivery aspects. The latter includes the need for a vector or specific delivery system, the choice of route of administration and the nature of the target animal population and their habitat. This review describes the formulation and delivery aspects of various types of antigens such as killed microorganisms, proteins and nucleic acids for the development of efficacious and safe animal vaccines. It also focuses on the challenges associated with the different approaches that might be required for formulating and delivering species specific vaccines, particularly if their intended use is for improved animal management with respect to disease and/or reproductive control.

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“…These include male-fertility-associated antigens, such as PH20, FA-1 and SP-10 (Suri 2004), and female-fertility-associated antigens, such as ZP1, ZP2 and ZP3 (Paterson et al 2000;Mackenzie et al 2006). Small peptides or linear arrays of peptides (polyepitopes) have also been trialled as immunocontraceptives (Miller et al 1997;Hardy et al 2002aHardy et al , 2002b. Of the sex-specific antigens, male antigens have proven less successful than female-fertility antigens, of which the most effective has been the zona antigen, ZP3.…”

Section: Alternative Antigen Targetsmentioning

confidence: 99%

Prospects for virally vectored immunocontraception in the control of wild house mice (Mus domesticus)

et al. 2007

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The wild house mouse (Mus domesticus) is not native to Australia and was introduced from Europe with early settlement. It undergoes periodic population explosions or plagues, which place significant economic and social burdens on agricultural communities. Present control mechanisms rely on improvements to farm hygiene and the use of rodenticides. This review covers over a decade of work on the use of virally vectored immunocontraception (VVIC) as an adjunct method of controlling mouse populations. Two viral vectors, ectromelia virus (ECTV) and murine cytomegalovirus (MCMV) have been tested as potential VVIC vectors: MCMV has been the most widely studied vector because it is endemic to Australia; ECTV less so because its use would have required the introduction of a new pathogen into the Australian environment. Issues such as efficacy, antigen choice, resistance, transmission, species specificity and safety of VVIC are discussed. In broad terms, both vectors when expressing murine zona pellucida 3 (mZP3) induced long-term infertility in most directly inoculated female mice. Whereas innate and acquired resistance to MCMV may be a barrier to VVIC, the most significant barrier appears to be the attenuation seen in MCMV-based vectors. This attenuation is likely to prevent sufficient transmission for broad-scale use. Should this issue be overcome, VVIC has the potential to contribute to the control of house mouse populations in Australia.

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Assessment of the immunocontraceptive effect of a zona pellucida 3 peptide antigen in wild mice

Cited by 24 publications

References 31 publications

Molecular adjuvant interleukin-33 enhances the antifertility effect of Lagurus lagurus zona pellucida 3 DNA vaccine administered by the mucosal route

Molecular adjuvant interleukin-33 enhances the antifertility effect of Lagurus lagurus zona pellucida 3 DNA vaccine administered by the mucosal route

Formulation and delivery of vaccines: Ongoing challenges for animal management

Prospects for virally vectored immunocontraception in the control of wild house mice (Mus domesticus)

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