To quantify water flow occurring in filter beds employed during wastewater treatment, laboratory column tracer experiments were performed on natural low-cost adsorbents (charcoal, oyster shell, and argan nutshell) under saturated flow conditions. The columns were packed with 100 % of adsorbents and with multi-sandlayer (MSL) system, consisting of permeable layers of pozzolan alternated with mixed layers (sand + adsorbent + sawdust). The results showed a more uniform flow in 100 % adsorbents for charcoal and oyster shell compared to their respective MSL columns. Conversely, flow uniformity was slightly improved in MSL versus 100 % adsorbent for argan nutshell columns. The choice of the appropriated adsorbent is crucial for the improvement of flow uniformity and treatment efficiency.