Assessment of the Impact of Integrated Pollution on the Monitoring of Biodiversity in Fisheries

Abstract

Results are presented of the comparative analysis of frequency of erythroid cells with micronuclei and “tailed” nuclei and of the interphase nuclei indices of red blood cells in the peripheral blood of bester, a sturgeon hybrid. The differences are found in the frequency of occurrence of abnormal red blood cells between the two groups of fish kept in cages in natural water (river) and in ponds with water supplied through a sedimentation basin. Despite these differences, the number of erythrocytes with chromosomal abnormalities in the bester of both groups is close to normal. However, the number of erythroid cells with micronuclei and “tailed nuclei” prevailed in the fish held in river cages. It was suggested that comparative cytogenetic monitoring of fish can be used not only to characterize the environment of water bodies, but also promote the rational organization of artificial reproduction of aquatic organisms that would contribute to the conservation of biodiversity of aquatic biological resources.

Basics of Methodology of Creating and Developing the Class of Natural Engineering Systems in Water Resources Management

“…We should note that the core and dominant operational principle among all of the identified system principles in the considered class of systems is the paramount role of the whole that determines the control from outside over the interaction and relations among the system's components [3,29,30,31,32,16,20].…”

Could scoring tailed and dumbbell-shaped nuclei increase the sensitivity of micronucleus analysis as a biomarker of radiation exposure?

“…To date the analysis of tailed and dumbbell-shaped nuclei in human biomonitoring, particularly for individuals living at high background radiation area (HBRA), is very rarely conducted. This contrasts with the fact that tailed nuclei are observed not only in human lymphocytes but also in erythrocytes of embryonic fish exposed to radiation in vivo (Braham et al 2017, Khetsuriani et al 2019, Khatun et al 2021. For this reason, the "erythrocyte micronucleus cytome assay" (ECMNA) has been used in aquatic cytogenotoxicity (Anbumani & Mohankumar 2011).…”