Assessment of the impact of three pesticides on microbial dynamics and functions in a lab-to-field experimental approach

Karas, Panagiotis A.; Baguelin, Céline; Pertile, Giorgia; Papadopoulou, Evangelia S.; Nikolaki, Sofia; Storck, Véronika; Ferrari, Federico; Trevisan, Marco; Ferrarini, Andrea; Fornasier, Flavio; Vasileiadis, Sotirios; Tsiamis, George; Martin-Laurent, Fabrice; Karpouzas, Dimitrios G.

doi:10.1016/j.scitotenv.2018.05.073

Cited by 75 publications

(35 citation statements)

References 63 publications

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“…Holophaga are homoacetogenic bacteria that degrades methoxylated aromatic compounds which are natural products of plants, animals and microorganisms (Liesack et al, 1994), however, more investigations are necessary to determine the reason of such difference. The stability of soil microbiome composition is very important for N and S cycles but certain pesticides and other chemicals may affect the composition of bacteria therefore, making serious ecological disturbances in living ecosystems (García-Delgado et al, 2018; Karas et al, 2018). At the same time there are some data that application of different herbicides including glyphosate, glufosinate, paraquat, paraquat-diquat and triasulfuron had no effect on the diversity and structure of soil bacteria and archaea (Pose-Juan et al, 2017; Dennis et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Microbial Diversity and Antimicrobial Resistance Profile in Microbiota From Soils of Conventional and Organic Farming Systems

et al. 2019

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Soil is one of the biggest reservoirs of microbial diversity, yet the processes that define the community dynamics are not fully understood. Apart from soil management being vital for agricultural purposes, it is also considered a favorable environment for the evolution and development of antimicrobial resistance, which is due to its high complexity and ongoing competition between the microorganisms. Different approaches to agricultural production might have specific outcomes for soil microbial community composition and antibiotic resistance phenotype. Therefore in this study we aimed to compare the soil microbiota and its resistome in conventional and organic farming systems that are continually influenced by the different treatment (inorganic fertilizers and pesticides vs. organic manure and no chemical pest management). The comparison of the soil microbial communities revealed no major differences among the main phyla of bacteria between the two farming styles with similar soil structure and pH. Only small differences between the lower taxa could be observed indicating that the soil community is stable, with minor shifts in composition being able to handle the different styles of treatment and fertilization. It is still unclear what level of intensity can change microbial composition but current conventional farming in Central Europe demonstrates acceptable level of intensity for soil bacterial communities. When the resistome of the soils was assessed by screening the total soil DNA for clinically relevant and soil-derived antibiotic resistance genes, a low variety of resistance determinants was detected (resistance to β-lactams, aminoglycosides, tetracycline, erythromycin, and rifampicin) with no clear preference for the soil farming type. The same soil samples were also used to isolate antibiotic resistant cultivable bacteria, which were predominated by highly resistant isolates of Pseudomonas, Stenotrophomonas, Sphingobacterium and Chryseobacterium genera. The resistance of these isolates was largely dependent on the efflux mechanisms, the soil Pseudomonas spp. relying mostly on RND, while Stenotrophomonas spp. and Chryseobacterium spp. on RND and ABC transporters.

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Section: Discussionmentioning

confidence: 99%

Microbial Diversity and Antimicrobial Resistance Profile in Microbiota From Soils of Conventional and Organic Farming Systems

et al. 2019

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“…Similarly, Papadopoulou et al (10) showed that quinone imine, the oxidized TP of ethoxyquin (an antioxidant used in the fruit-packaging industry) was equally as toxic as, or more toxic than, the parent compound to ammonia-oxidizing microorganisms (AOM). This group has been shown to be particularly sensitive to environmental perturbations (11) including pesticide exposure (6,9).…”

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confidence: 99%

Blame It on the Metabolite: 3,5-Dichloroaniline Rather than the Parent Compound Is Responsible for the Decreasing Diversity and Function of Soil Microorganisms

Vasileiadis

Puglisi

Papadopoulou

et al. 2018

Appl Environ Microbiol

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Pesticides are key stressors of soil microorganisms with reciprocal effects on ecosystem functioning. These effects have been mainly attributed to the parent compounds, while the impact of their transformation products (TPs) has been largely overlooked. We assessed, in a meadow soil (A),the transformation of iprodione and its toxicity on the (i) abundance of functional microbial groups, (ii) activity of key microbial enzymes and (iii) diversity of bacteria, fungi and ammonia-oxidizing microorganisms (AOM) using amplicon sequencing. 3,5-Dichloroaniline (3,5-DCA), the main iprodione TP, was identified as key explanatory factor for the persistent reduction in enzymatic activities and potential nitrification (PN), and for the observed structural changes in the bacterial and fungal community. The abundance of certain bacterial (, , and ) and fungal () groups were negatively correlated with 3,5-DCA. A subsequent study in a fallow agricultural soil (B) showed a limited formation of 3,5-DCA which concurred with the lack of effects on nitrification. Direct 3,5-DCA application in soil B induced a dose-dependent reduction of PN and NO-N, which recovered with time. assays with terrestrial AOM verified the greater toxicity of 3,5-DCA over iprodione. Nitrosotalea sinensis Nd2 was the most sensitive AOM to both compounds. Our findings build on previous evidence on the sensitivity of AOM to pesticides reinforcing their potential utilization as indicators of the soil microbial toxicity of pesticides in pesticide environmental risk analysis and stressing the need to consider the contribution of TPs in the toxicity of pesticides on the soil microbial community. Pesticide toxicity on soil microorganisms is an emerging issue in pesticide risk assessment, dictated by the pivotal role of soil microorganisms on ecosystem services. However, the focus has traditionally been on parent compounds, while transformation products (TPs) are largely overlooked. We tested the hypothesis that TPs can be major contributors on the soil microbial toxicity of pesticides, using, iprodione, and its main TP, 3,5-dichloroaniline, as model compounds. We demonstrated, by measuring functional and structural endpoints, that 3,5-dichloraniline and not iprodione was associated with adverse effects on soil microorganisms, with nitrification being mostly affected. Pioneering assays with relevant ammonia-oxidizing bacteria and archaea verified the greater toxicity of 3,5-dichloraniline. Our findings are expected to advance environmental risk assessment highlighting the potential of ammonia-oxidizing microorganisms as indicators of the soil microbial toxicity of pesticides and stressing the need to consider the contribution of TPs on pesticides soil microbial toxicity.

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“…Finally, the increase in urease activity from 0 to 7 then from 7 to 60 days may show that isoproturon metabolites would have similar effect on urease than isoproturon (Figures 2 and 3). Karas et al [34] evidenced that monodemethyl-isoproturon and didemethyl-isoproturon inhibited various enzyme activities while isoproturon did not, but the urease activity was not considered. In amended soils, on the contrary, isoproturon and its metabolites had no effect on the urease activity either in compacted and not compacted soils (p > 0.05) (Figure 4 and Table 3).…”

Section: Conditions Of Soil Incubationmentioning

confidence: 99%

“…It decreases the dehydrogenase, acid and alkaline phosphomonoesterases, and leucine aminopeptidase activities [27,31,32]. By contrast, it increases the urease activity [33], but it had no effect on several other activities such as arylsulfatase, beta-glucosidase or esterase [20,34]. Mamy et al [20] also showed that Soil enzyme activities are known to be relevant indicators of soil health, and they were successfully used to discriminate a wide range of agricultural practices such as organic amendments or pesticides addition, but also perturbation at the landscape scale [15].…”

Section: Introductionmentioning

confidence: 99%

Effect of Multiple Stresses, Organic Amendment and Compaction, on the Fate and Impact of Isoproturon in Soil

et al. 2020

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Organic matter decline and compaction are two major processes of soil degradation. Organic amendment is a current practice to compensate the loss of organic matter, which could in addition contribute to increase soil aggregate stability and limit compaction. Therefore, the objective of this work was to study the effect of multiple physico-chemical stresses, organic amendment (compost of sewage sludge and green waste) addition and soil compaction, on the fate and impact (measured through the urease enzyme activity) of isoproturon. Compost addition and compaction did not significantly affect the fate and impact of isoproturon. The lack of effect of compost can be due to the delay between soil sampling and soil amendment. Compaction had no effect probably because the porosity reduction does not affect the habitable pore space accessible to degrading microorganisms. Nevertheless, isoproturon significantly increased the urease enzyme activity in compacted and not compacted unamended soils contrary to the amended ones. It seems that the organic amendment could act as a buffer with regards to the impact of isoproturon. The results obtained in this work suggest that, in general, the fate and impact of isoproturon in soils will not change following compaction and/or organic amendment addition, neither the corresponding risks for the environment.

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Assessment of the impact of three pesticides on microbial dynamics and functions in a lab-to-field experimental approach

Cited by 75 publications

References 63 publications

Microbial Diversity and Antimicrobial Resistance Profile in Microbiota From Soils of Conventional and Organic Farming Systems

Microbial Diversity and Antimicrobial Resistance Profile in Microbiota From Soils of Conventional and Organic Farming Systems

Blame It on the Metabolite: 3,5-Dichloroaniline Rather than the Parent Compound Is Responsible for the Decreasing Diversity and Function of Soil Microorganisms

Effect of Multiple Stresses, Organic Amendment and Compaction, on the Fate and Impact of Isoproturon in Soil

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