Assessment of the in vitro anti-lipid peroxidative activity of Costus afer stem extract

Abstract

Objective: Costus afer is a perennial monocot consumed as a remedy for various ailments. The present study investigates the effect of C. afer stem extract on ferrous/ascorbate-induced lipid peroxidation in rat brain, liver, and kidney homogenates. The inherent antioxidant and free radical scavenging potentials of the extract were also assessed using various in vitro models. Methods: Homogenates of isolated tissues were prepared, and lipid peroxidation was induced using the ferrous/ascorbate system and subsequently incubated with various concentrations of the extract. The ability of the methanolic extract to scavenge hydroxyl radicals, hydrogen peroxide and its ability to reduce ferric ions were investigated. The total antioxidant potential through the phosphomolybdate method was also measured. Results: The results revealed that C. afer stem extract reduced tissue lipid peroxidation induced by ferrous/ascorbate. The extract also possessed significant antioxidant/ free radical scavenging activity through the other in vitro models. In all cases, the effects were concentration dependent. Conclusion: The methanolic extract of C. afer stem possessed significant antioxidant potential ascribable to important phytochemicals and could protect cells/tissues from reactive oxygen species-induced damage. Thus, the plant has high pharmacological and ethnobotanical prospects.

Review of Phyto-Medical Extracts’ and Compounds’ Anti-Radiation Properties

“…afer is a perennial rhizomatous monocot distributed in the moist and shady forest belts of Africa, belonging to the family Costaceae Jilani and Packirisamy [70]. It has been shown to protect against testicular damage induced by lead [71], prevent lipid peroxidation in the liver, kidney, and brain induced by ferrous/ascorbate [72], and exhibit anti-sickling properties [73]. The biological activity may be attributed to the presence of diverse phytochemicals such as terpenoids, alkaloids, cardiac glycosides, phenols, and tannins [74].…”

Assessment of the Inherent in-vitro Antioxidant Potential of Commelina benghalensis Leaf Extract

“…The anti-lipid peroxidation activity of extract on tissue homogenates was performed according to the method of Yoshiyuki et al [10] with some modifications. Homogenates were prepared from fresh bovine liver and egg yolk as described [11]. Homogenate (0.5 mL) was mixed with 0.1 ml of 0.04 M ferrous chloride, 0.1 ml of 0.1 mM ascorbic acid, 0.2 ml of 0.02 M phosphate buffer (pH 7.4) and 0.5 mL of either extract or control (quercetin monohydrate).…”