Hot-dip galvanized components offer a great potential for corrosion protection of up to 100 years, while laser beam welding in vacuum (LaVa) has the advantage of high penetration depths Combined, this process chain can be economically used in steel construction of bridges, wind turbines, or other steel constructions. Therefore, investigations of butt joint welding of galvanized 20 mm thick S355M steel plates using LaVa were carried out. The butt joints were prepared under different cutting edges such as flame-cut, sawn, and milled edges, and they were studied with and without the zinc layer in the joint gap. For this purpose, the laser parameters such as the beam power, welding speed, focus position, and working pressure all varied, as did the oscillation parameters. The welds performed using an infinity oscillation with an amplitude of 5 mm represented a pore-free weld up to a zinc layer thickness of 400 µm in the joint gap. The seam undercut increased with increasing the zinc layer thickness in the joint gap, which can be explained by the evaporating zinc and consequently the missing material, since no filler material was used. The joint welds with zinc only on the sheet surface achieved a sufficient weld quality without pores.