Assessment of the neuroprotective effect of selenium-loaded chitosan nanoparticles against silver nanoparticles-induced toxicity in rats

Shalaby, Omnia E.; Ahmed, Yasmine H.; Mekkawy, Aya M.; Mahmoud, Mohamed Y.; Khalil, Heba M. A.; El-Bargeesy, G. A.

doi:10.1016/j.neuro.2023.02.009

Cited by 9 publications

(5 citation statements)

References 77 publications

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“…64 The present investigation induced astrocytic activation, as evidenced by the upregulation (overexpression) of GFAP in various brain regions, including the cerebral cortex, cerebellar cortex, and hippocampus of rats assigned to group II, as depicted in Figure 6B,F,J, in comparison to the control rats assigned to group I which revealed mild immunoreactivity to GFAP, as depicted in Figure 6A,E,I. These results come in agreement with the findings obtained by Shalaby et al 65 in brains of 40 adult female albino rats exposed to silver NPs at a dose of 100 mg/ kg for 2 months. Astrocytes play a critical role in maintaining neuronal viability, preserving the integrity of the BBB, regulating vascular reactivity, facilitating energy metabolism, controlling extracellular glutamate levels, and safeguarding against ROS.…”

Section: Immunohistochemical Investigations 2321 Glial Fibrillary Aci...supporting

confidence: 93%

Neuroprotective Assessment of Betaine against Copper Oxide Nanoparticle-Induced Neurotoxicity in the Brains of Albino Rats: A Histopathological, Neurochemical, and Molecular Investigation

Hashim,

Bashir,

Rashad

et al. 2024

ACS Chem. Neurosci.

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Copper oxide nanoparticles (CuO-NPs) are commonly used metal oxides. Betaine possesses antioxidant and neuroprotective activities. The current study aimed to investigate the neurotoxic effect of CuO-NPs on rats and the capability of betaine to mitigate neurotoxicity. Forty rats; 4 groups: group I a control, group II intraperitoneally CuO-NPs (0.5 mg/kg/day), group III orally betaine (250 mg/kg/day) and CuO-NPs, group IV orally betaine for 28 days. Rats were subjected to neurobehavioral assessments. Brain samples were processed for biochemical, molecular, histopathological, and immunohistochemical analyses. Behavioral performance of betaine demonstrated increasing locomotion and cognitive abilities. Group II exhibited significantly elevated malondialdehyde (MDA), overexpression of interleukin-1 beta (IL-1β), and tumor necrosis factor-alpha (TNF-α). Significant decrease in glutathione (GSH), and downregulation of acetylcholine esterase (AChE), nuclear factor erythroid 2-like protein 2 (Nrf-2), and superoxide dismutase (SOD). Histopathological alterations; neuronal degeneration, pericellular spaces, and neuropillar vacuolation. Immunohistochemically, an intense immunoreactivity is observed against IL-1β and glial fibrillary acidic protein (GFAP). Betaine partially neuroprotected against CuO-NPs associated alterations. A significant decrease at MDA, downregulation of IL-1β, and TNF-α, a significant increase at GSH, and upregulation of AChE, Nrf-2, and SOD. Histopathological alterations partially ameliorated. Immunohistochemical intensity of IL-1β and GFAP reduced. It is concluded that betaine neuroprotected against most of CuO-NP neurotoxic effects through antioxidant and cell redox system stimulating efficacy.

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Section: Immunohistochemical Investigations 2321 Glial Fibrillary Aci...supporting

confidence: 93%

Neuroprotective Assessment of Betaine against Copper Oxide Nanoparticle-Induced Neurotoxicity in the Brains of Albino Rats: A Histopathological, Neurochemical, and Molecular Investigation

Hashim,

Bashir,

Rashad

et al. 2024

ACS Chem. Neurosci.

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“…In the present study, AgNPs treated group showed significant increase in the lipid peroxidation marker (MDA) level, while the cellular antioxidant enzyme system (SOD, CAT and GSH) levels were significantly decreased. Previous studies reported the same findings [31,32,33], indicating that one of the main mechanisms associated with AgNPs toxicity is ROS generation that leads to oxidative damage of the cell. According to Nie et al [34], silver ions (Ag+) are responsible for AgNPs toxicity, which are released from the nanoparticles upon cell entry, inducing ROS production.…”

Section: Discussionsupporting

confidence: 66%

Evaluation of reproductive capacity in adult male rats exposed to silver nanoparticles and the ameliorative role of lycopene

Fouad,

El-Dakdoky,

Hassan

2023

Journal of Scientific Research in Science

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The present study was aimed to assess reproductive capacity of male albino rats exposed to silver nanoparticles (AgNPs) and the possible ameliorating role of lycopene (LYC). Twenty eight male albino rats were divided as follows: group I, received distilled water; group II received 100 mg/kg BW AgNPs; group III received 5 mg/kg BW LYC and group IV received both AgNPs+LYC at their same dosage. All doses were administrated orally and daily for 60 days. The results showed that AgNPs exposure decreased body weight gain as well as testicular and epididymal weight, reduced sperm count and induced sperm malformations. Histological findings revealed histo-morphometric change to the seminiferous tubules in AgNPs group. Biochemical investigation showed that AgNPs increased MDA level and decreased SOD, GSH and CAT levels in testicular tissue. Also it increased testicular ALP level and decreased testicular SDH level. Immuno-histochemical staining showed an increased caspase-3 and TNFα expression in AgNPs group. Meanwhile, lycopene co-treated rats showed improvements in the body weight, testicular and epididymal weight status, sperm characteristics, histological architecture, oxidative, inflammatory and apoptotic biomarkers.

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“…These findings were in agreement with a previous study (He et al 2014) who demonstrated the toxic effects of SeNPs at doses higher than 4mg/kg on the reproductive organs of rats. On the other hand, administration of low doses of SeNPs ranging from 0.4 to 2mg/kg has a reported beneficial activity on different organs, including brain (Bashir et al 2021;Shalaby et al 2023) and reproductive organs (Hozyen et al 2020).…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo Antibacterial and Antibiofilm Efficacy of Selenium Nanoparticles against Staphylococcus aureus Supported with Toxicopathological and Behavioral Studies in Rats

2023

Int J Vet Sci

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The present study aimed to investigate the antibacterial and antibiofilm effects of selenium nanoparticles (SeNPs) synthesized by two different methods against Staphylococcus aureus forming biofilm in vivo and in vitro, with a focus on the toxicological and behavioral changes of various SeNPs concentrations in rats. SeNPs were prepared by green and chemical methods and characterized by X-ray diffraction (XRD), Transimission electron microscope TEM. Their antibacterial efficacy was evaluated by agar well diffusion test, also minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) values were detected. Experimental design sixty female lactating rats were divided into 6 groups: G1 control negative, G2 control positive, G3-G6 infected animals received SeNPs with different concentrations. A bacteriological and histopathological examination of the mammary gland was carried out, an antioxidants assay was evaluated and finally a toxicological study was done. Green synthesized SeNPs showed antibacterial effects at different concentrations (600 μg/mL to 10μg/mL) with MIC and MBC values of 10μg/mL and 25μg/mL. Time kill kinetics showed that SeNPs inhibited the growth of S. aureus completely after 4 hours. The treated group showed pronounced improvement in the main lactiferous duct with normal histological structure compared with the infected group without treatment; by increasing dose the main lactiferous duct structure and acini became nearly identical to the control negative group. Bacteriological examination showed a complete absence of bacterial colonies in all tested concentrations in female rats. The highest doses of SeNPs showed mild cytoplasmic vacuolization of some hepatocytes with activation of kupffer cells with normal hepatocytes structure compared to the control group, a decrease in catalase (CAT) and total antioxidant capacity (TAC) in most examined doses compared to the control group, and an increase in superoxide dismutase (SOD) level in all treated animal. SeNP supplementation could be a safe and helpful treatment for Staphylococcus aureus infection. It prevents biofilms formation both in vitro and in vivo, and it had no notable toxicological or behavioral effects on rats.

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Assessment of the neuroprotective effect of selenium-loaded chitosan nanoparticles against silver nanoparticles-induced toxicity in rats

Cited by 9 publications

References 77 publications

Neuroprotective Assessment of Betaine against Copper Oxide Nanoparticle-Induced Neurotoxicity in the Brains of Albino Rats: A Histopathological, Neurochemical, and Molecular Investigation

Neuroprotective Assessment of Betaine against Copper Oxide Nanoparticle-Induced Neurotoxicity in the Brains of Albino Rats: A Histopathological, Neurochemical, and Molecular Investigation

Evaluation of reproductive capacity in adult male rats exposed to silver nanoparticles and the ameliorative role of lycopene

In vitro and in vivo Antibacterial and Antibiofilm Efficacy of Selenium Nanoparticles against Staphylococcus aureus Supported with Toxicopathological and Behavioral Studies in Rats

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