With the accuracy and convenience improvement of electromyographic (EMG) acquired by wearable devices, EMG is gradually used to evaluate muscle force signal, a non-invasive evaluation method. However, the relationship between EMG and force is a complex nonlinear relationship, even which will change with different movements and different muscle states. Therefore, it is difficult to evaluate this nonlinear EMG–force relationship, especially when the muscle state gradually transits from non-fatigue to deep fatigue. For more accurate values of force in human fatigue state, this paper proposes a dual-input Laguerre–Volterra network (LVN) model based on ant colony optimization. First, the changes in 19 EMG features are discussed with increasing fatigue. We also consider two non-Gaussian features: kurtosis and negentropy in the 19 features. Later, 11 EMG fatigue features are picked out according to the fatigue test. Then, the preprocessed EMG and a composite signal of the 11 fatigue features are simultaneously input into the LVN model. Subsequently, the ant colony optimization algorithm is selected to train the model parameters. At the same time, a penalty term that we defined is introduced into the model cost function to adjust the weight of each feature adaptively. Finally, some experiments prove that the LVN model could quick fit the accurate force signal in five fatigue stages, such as non-fatigue, slight fatigue, mild fatigue, severe fatigue, and extreme fatigue. This LVN model can quickly transform EMG into strength signal in real time, which is suitable for people to observe muscle strength by a wearable device and makes it easy to detect the muscle current state. This model has good stability and can remain effective for a long time with training once, which provides convenience for the users of wearable devices.