Assessment of the potential of the multi‐enzyme producer <i>Bacillus amyloliquefaciens</i> US573 as alternative feed additive

Farhat-Khemakhem, Ameny; Blibech, Monia; Boukhris, Ines; Makni, Mohamed; Chouayekh, Hichem

doi:10.1002/jsfa.8574

Cited by 28 publications

(31 citation statements)

References 59 publications

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“…Our data were in agreement with the previous studies of Farhat‐Khemakhem et al. and Murugesan et al. , where Bacillus amyloliquefaciens US573, B. subtilis , and B. coagulans have been reported to exhibit the highest growth at the initial pH of 7.0.…”

Section: Resultssupporting

confidence: 94%

“…All isolates showed highest growth ability at pH values that ranged from 5 to 9 over the 24 H growth duration, with a maximum growth at pH 7. Our data were in agreement with the previous studies of Farhat-Khemakhem et al [10] and Murugesan et al [38], where Bacillus amyloliquefaciens US573, B. subtilis, and B. coagulans have been reported to exhibit the highest growth at the initial pH of 7.0. In fact, Bacillus species are known to grow at wide pH values ranging from 5 to 9 and some even at pH as low as 2 or as high as 10.…”

Section: Screening For Probiotic Properties 341 Growth At Differensupporting

confidence: 94%

“…The fermentation profile of each strain was assayed using the kit API 50 CHB system in line with the manufacturer's protocol (Bio kit Merieux, France) as described by Ghanbari et al [21]. The lack of indesirable enzymatic activities was checked by the API ZYM system (BioMerieux) as described by Farhat-Khemakhem et al [10].…”

Section: Carbohydrate Fermentation Profile and Enzyme Activitiesmentioning

confidence: 99%

“…The five selected strains were checked for their aptitude to initiate growth under acidic and alkaline pH culture conditions as well as in the presence of bovine bile [22]. The study was carried out as reported by Farhat-Khemakhem et al [10].…”

Section: Screening For Probiotic Properties 291 Growth At Differenmentioning

confidence: 99%

“…The efficiency of vegetative cells of the isolates US134, US150, US176, US180, and US191 with regard to in vitro wheat dry matter digestibility was evaluated in comparison with the approved commercial feed additive Rovabio R Max. Assays were performed in triplicate in accordance with the protocol described by Farhat-Khemakhem et al [10].…”

Section: In Vitro Wheat Dry Matter Digestibilitymentioning

confidence: 99%

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Selection ofBacillus subtilisUS191 as a mannanase‐producing probiotic candidate

Blibech

Mouelhi

Farhat-Khemakhem

et al. 2019

Biotech and App Biochem

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β‐Mannanases are crucial enzymes for the breakdown of mannans. As Mannans are being considered as antinutritional factors in poultry production, the search for mannanase‐producing probiotic bacteria is now attracting considerable attention as a strategy to enhance nutrients bioavailability. Five soil born Bacilli (US134, US150, US176, US180, and US191) were selected for their ability to produce extracellular β‐mannanases that were biochemically characterized. The probiotic properties of these strains were determined to assess their potential as animal feed supplements. Bacillus subtilis US191 was shown to be sensitive to all antibiotics tested, to inhibit growth of the bacterial pathogens tested, and to produce a thermostable β‐mannanase. It exhibited a notable acid and bovine bile tolerance and high ability to form biofilm. These features may favor its adherence to the intestinal epithelial cells allowing its survival and persistence in the digestive tract. Furthermore, our study revealed that US191 was among the strains showing the highest ability to digest wheat dry matter in vitro when compared to the commercial feed additive Rovabio® Max. Altogether, our findings suggest that the β‐mannanase producer B.subtilis US191 is a promising probiotic candidate for the feed industry.

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Section: Resultssupporting

confidence: 94%

Section: Screening For Probiotic Properties 341 Growth At Differensupporting

confidence: 94%

Section: Carbohydrate Fermentation Profile and Enzyme Activitiesmentioning

confidence: 99%

Section: Screening For Probiotic Properties 291 Growth At Differenmentioning

confidence: 99%

Section: In Vitro Wheat Dry Matter Digestibilitymentioning

confidence: 99%

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