Assessment of the potential of SiPM-based systems for bioluminescence detection

Lomazzi, Samuela; Caccia, M.; Distasi, Carla; Dionisi, Marianna; Lim, Dmitry; Martemiyanov, A.; Nardo, Luca; Santoro, R.

doi:10.1016/j.nima.2020.164493

Cited by 3 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In any case, the correct assessment of the peak values does not correspond to faithful reproduction of the Ca 2+ signal temporal profile, as made apparent by direct comparison of the traces in the CI and SPC branches in this concentration range (see Supporting Informatio, Figure S5 ). More details about the pile-up effect and the limitations of both the modalities are reported in ref ( 25 ).…”

Section: Resultsmentioning

confidence: 99%

“…See the Supporting Information for the detailed specifics of the twin gain amplifier and a schematic of the circuit (Figure S1). In order to enhance the SPC capability of the system, the 34 dB amplified signal was shortened by means of a zero-pole cancellation circuit, reaching a full-time development of 30 ns (see ref 25 and the Supporting Information for further details). This corresponded to a 5% probability of pile-up events at 2 MHz count rate assuming a Poissonian temporal distribution of impinging photons.…”

Section: ■ Instrumentation Materials and Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Assessment of a Silicon-Photomultiplier-Based Platform for the Measurement of Intracellular Calcium Dynamics with Targeted Aequorin

et al. 2020

View full text Add to dashboard Cite

Ca 2+ is among the most important intracellular second messengers participating in a plethora of biological processes, and the measurement of Ca 2+ fluctuations is significant in the phenomenology of the underlying processes. Aequorin-based Ca 2+ probes represent an invaluable tool for reliable measurement of Ca 2+ concentrations and dynamics in different subcellular compartments. However, their use is limited due to the lack on the market of ready-to-use, cost-effective, and portable devices for the detection and readout of the low-intensity bioluminescence signal produced by these probes. Silicon photomultipliers (SiPMs) are rapidly evolving solid-state sensors for low light detection, with single photon sensitivity and photon number resolving capability, featuring low cost, low voltage, and compact format. Thus, they may represent the sensors of choice for the development of such devices and, more in general, of a new generation of multipurpose bioluminescence detectors suitable for cell biology studies. Ideally, a detector customized for these purposes must combine high dynamic range with high fidelity in reconstructing the light intensity signal temporal profile. In this article, the ability to perform aequorin-based intracellular Ca 2+ measurements using a multipurpose, low-cost setup exploiting SiPMs as the sensors is demonstrated. SiPMs turn out to assure performances comparable to those exhibited by a custom-designed photomultiplier tube-based aequorinometer. Moreover, the flexibility of SiPM-based devices might pave the way toward routinely and wide scale application of innovative biophysical protocols.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: ■ Instrumentation Materials and Methodsmentioning

confidence: 99%

Assessment of a Silicon-Photomultiplier-Based Platform for the Measurement of Intracellular Calcium Dynamics with Targeted Aequorin

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Combining the robustness of avalanche photodiodes and unmatched photon number resolution capability with high quantum efficiency and wide sensitive areas, SiPMs replaced PMTs in a high number of applications [59][60][61]. Thanks to a very high gain (10 6 vs 100-200 of avalanche photodiodes)…”

Section: When Citing Please Refer To the Published Versionmentioning

confidence: 99%